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    --- title: Modules Team - nf-core Hackathon March 2023 tags: march-2023,nf-core,hackathon,progress description: View the slide with "Slide Mode". --- <!-- .slide: data-background="https://raw.githubusercontent.com/maxulysse/maxulysse.github.io/main/assets/img/svg/green_white_bg.svg" --> <a href="https://www.nf-co.re"><img src="https://i.imgur.com/hEDjpCs.png" width="65%"><img></a> ### 🚀 Modules Team 🚀 _27th-29th March 2023_ The Hackathon March 2023 [Project Board](https://github.com/orgs/nf-core/projects/38) The Group Discussion Channel `#hackathon-march-2023-modules` <img src="https://openmoji.org/data/color/svg/E040.svg" width=50> @nf-core / <img src="https://openmoji.org/data/color/svg/E045.svg" width=50> @nf-core --- # Modules Team --- # Team Leads - General advice, pointers, co-ordination etc. - Maxime Garcia (@maxulysse) - Matthias De Smet (@matthdsm) - Harshil Patel (@drpatelh) - Jonathan Manning (@pinin4fjords) - Nicolas Vannieuwkerke (@nvnieuwk) --- - Highly-multiplexed imaging subgroup - Adam Taylor (@adamjtaylor) - Florian Wuennemann (@FloWuenne) _But always ask first in: `#hackathon-march-2023-modules`!_ --- # Tasks Ideas - General development work - New modules - Fix modules tests - Improve modules --- - Highly-multiplexed imaging / Spatial Omics - Add new modules geared at this type of data analsysis, starting with porting core modules of [MCMICRO](https://mcmicro.org/) - Please label issues `imaging` in Github - [Imaging specific project board view](https://github.com/orgs/nf-core/projects/38/views/7?filterQuery=repo%3A%22nf-core%2Fmodules%22+label%3A%22new+module%22++label%3Aimaging) --- # Remember - Add yourself to Team Members slide - Assign yourself on issues on the [project board](https://github.com/orgs/nf-core/projects/38) - Post your progress here **daily**! - Sign up for a reviewing buddy (see slide)! --- # Team Members <div class="multi-column"> <div> - [Maxime Garcia](@maxulysse) - [Verena Kutschera](@verku) - [Adrien Coulier](@Aratz) - [Bojan Balic](@boleodole) - [Darko Cucin](@darko-cucin) - [Remi-Andre Olsen](@remiolsen) - [Sandro Carollo](@sandrocarollo) </div> <div> - [Georgios Fotakis](@abyssum) - [Valentin Marteau](@vjmarteau) - [Marie Lataretu](@MarieLataretu) - [Fynn Freyer](@FynnFreyer) - [Toni Hermoso Pulido](@toniher) - [Paul Wolk](@paulwolk) - [Roger Olivella](@rolivellla) - [Maulik Upadhyay](@BioInf2305) </div> </div> --- <div class="multi-column"> <div> - [Lili Andersson-Li](@Lili) - [Joan Gaztelu](@LlaneroHiboreo) - [Narci](@kubranarci) - [Aleksandra Vitkovac](@alexiandra97) - [Anabella Trigila](@atrigila) - [Mariangela Santorsola](@msantorsola) - [Florian Heyl](@heylf) </div> <div> - [Milena Amidzic](@milenaamidzic) - [Gordon Draskic](@gordondraskic) - [Vojislav Lukic](@VojislavLukic) - [Alfred Kedhammar](@kedhammar) - [Louisa Perelo](@louperelo) - [Ferriol Calvet](@ferriolcalvet) - [Federica Brando](@FedericaBrando) </div> </div> --- ## Highly-multiplexed imaging subgroup <div class="multi-column"> <div> - [Florian Wuennemann](@FloWuenne) - [Adam Taylor](@adamjtaylor) - [Margot Chazotte](@MargotCh) - [Jose Nimo](@josenimo) - [Chiara Schiller](@chiarasch) - [Kresimir Bestak](@kbestak) </div> <div> - [Aroj Hada](@ArozHada) - [Miguel A. Ibarra-Arellano](@migueLib) - [Victor Perez](@VictorDidier) - [Wouter-Michiel Vierdag](@melonora) - [Luis Kuhn Cuellar](@luiskuhn) - [Carolin Schwitalla ](@CaroAMN) - [Dominik Molitor](@DojakM) - [Jelena Pejovic Simeunovic](@JPejovicApis) - [Svetozar Nesic](@snesic) - [Emilio Palumbo](@emi80) </div> </div> --- # Reviewing Buddy List <div class="multi-column"> <div> | Person A | Person B | |-------------|----------| | Bojan | Darko | | Kresimir | Chiara | | Aroj | Margot | | Jose | Florian | | Gordon | Vojislav | | Vojislav | Gordon | </div> <div> | Person A | Person B | |-------------|----------| | Fynn | Paul | | Marie | Fynn | | Paul | Marie | | Verena | Alfred | | Louisa | Jasmin | </div> </div> --- # Day One - Imaging I/IV <small> - 🧡 [#3090 - Background subtraction](https://github.com/nf-core/modules/issues/3090) (KB) - [Monday, 3.30 CET] Assigned and in progress but no linked branch/PR CET - [EOD] figuring out passing optional inputs such as px size - [Tuesday AM] Working from nf-core but test dataset does not have the right metadata. - Small tonsil test data should work - Need to add columns to it - 🧡 [#3089 - BaSicPy](https://github.com/nf-core/modules/issues/3089) (VP) - [Monday, 3.30 CET] Assigned and in progress but no linked branch/PR CET - [EOD] looking at passing optional inputs. Issues with docker container working - [Tuesday AM] - 🧡 [#3076 - Deepcell/Mesmer](https://github.com/nf-core/modules/issues/3076) (CS, MI) - [Monday, 3.30 CET] Assigned and in progress but no linked branch/PR CET - [EOD] Passes testing with local data - Detail work for optional inputs ongoing - [Tuesday AM] Need image with membrane and nuclear marker (does this work? https://github.com/nf-core/test-datasets/pull/821) </small> --- # Day One - Imaging II/IV <small> - 🧡 [#3065 - Coreograph](https://github.com/nf-core/modules/issues/3065) (AH, MC) - [Monday, 3.30 CET] Assigned and in progress but no linked branch/PR CET - [EOD] Need to make bioconda package. Asking for help on this. (Really nice to have bioconda even if not strictly required) - To make seperate HackMD for technical details at this stage. Can skip bioconda tests manually - [Tuesday AM] Agree to skip bioconda for now with exceptions in test file,. Submit just DAPI from exemplar-001 as test-dataset - 🧡 [#3044 - Mcquant](https://github.com/nf-core/modules/issues/3044) (FW) - [Monday, 3.30 CET] Assigned and in progress but no linked branch/PR CET - Waiting on a suitable test-dataset from AT - [EOD] As above. Works on real data! - [Tuesday AM] Can test once test data is merged </small> --- # Day One - Imaging III/IV <small> - 💜 [#2864 - Ilastik/pixelclassification](https://github.com/nf-core/modules/issues/2864) (FW) - [Monday, 3.30 CET] Assigned and ready for review - PR [#2875](https://github.com/nf-core/modules/pull/2875) approved by Nicolas Vannieuwkerke - 16.10 CET Merged! - [EOD] Conda tests skipped as has its own coda channel - 💜 [#2865 - Ilastik/Multicut](https://github.com/nf-core/modules/issues/2865) (FW) - [Monday, 3.30 CET] Assigned and ready for review - PR [#2894](https://github.com/nf-core/modules/pull/2894) approved by Nicolas Vannieuwkerke, waiting for review by Maxime Garcia => Failing CI tests - [EOD] prettier lint failing. Request out on how to solve - 11.35 CET -> Merged </small> --- # Day One - Imaging IV/IV <small> - 🧡 [#2477 - Mindagap/Mindagap](https://github.com/nf-core/modules/issues/2477) (FW) - [Monday, 3.30 CET] Assigned and ready for review - PR [#2477](https://github.com/nf-core/modules/issues/2477) - Three reviewers assigned - Fails nf-core linting - [EOD] stub test needed - [Tuesday AM] Refactoring to add mandatory args - 🧡 [#3095 - SpatialData/MCMICRO](https://github.com/nf-core/modules/issues/3095) (WM, FW) - [Monday, 3.30 CET] Assigned and in progress but no linked branch/PR CET - [EOD] Docker image being built for this. Figuring out right output structure. Draft working - [Tues AM] Creating CLI tool. Looking for valid output directories. - 💚 [#3100 - Scimap/MCMICRO](https://github.com/nf-core/modules/issues/3100) (LKC, DM, CS) - [Monday, 16:00 CET] Worked on general structure - [EOD] First module attempt 🚀. Have basic structure but not working yet. Need to confirm right test data. - [Tues AM] Have positive results with test data. wiring parameters together. Used quantification test data. </small> --- # Day One - New Modules I <small> - [#2571 - MitoHiFi/findMitoReference](https://github.com/nf-core/modules/issues/2571) (@verku) - [#3103 - interproscan](https://github.com/nf-core/modules/issues/3103) (@toniher) - [#14 - RNA Velocity](https://github.com/nf-core/scrnaseq/issues/14) - [#3109 - chopper](https://github.com/nf-core/modules/issues/3109) (@FynnFreyer) - [PR](https://github.com/nf-core/modules/pull/3174) waiting for review - [#3105 - thermorawfileparser](https://github.com/nf-core/modules/issues/3105) (@rolivella) - [#3113 - SURVIVOR/FILTER](https://github.com/nf-core/modules/issues/3113) (@LlaneroHiboreo) - [#2572 - HyPo](https://github.com/nf-core/modules/issues/2572) (@remiolsen) - [#2937 - purecn/intervalfile](https://github.com/nf-core/modules/issues/2937) (@lbeltrame, @aldosr) - [#2938 - purecn/coverage](https://github.com/nf-core/modules/issues/2938) (@lbeltrame, @aldosr) - [#2939 - purecn/normaldb](https://github.com/nf-core/modules/issues/2939) (@lbeltrame, @aldosr) - [#2940 - purecn/run](https://github.com/nf-core/modules/issues/2940) (@lbeltrame, @aldosr) - [#3078 - sigprofilerextractor](https://github.com/nf-core/modules/issues/3078) (@ferriolcalvet) - [#3147 - plink/indeppairwise](https://github.com/nf-core/modules/issues/3147) (@atrigila) </small> --- # Day One - New Modules II <small> - [#2940 - purecn/dx](https://github.com/nf-core/modules/issues/2941) (@lbeltrame, @aldosr) - [#3087 - bioqc](https://github.com/nf-core/modules/issues/3087) (@boleodole) - [#1869 - wisecondorx/convert](https://github.com/nf-core/modules/issues/1869) (@nvnieuwk) - PR awaiting approval - [#3133 - sambamba/flagstat](https://github.com/nf-core/modules/issues/3133) (@kubranarci) - [#2957 - brackenbuild](https://github.com/nf-core/modules/issues/2957) (@darko-cucin) - [#3110 - NanoComp](https://github.com/nf-core/modules/issues/3110) (@paulwolk) - Monday: Getting familiar with nf-core modules and writing the base of the module - Tuesday: Testing - Wednesday: PR awaiting approval - [#3112 - ART/art_illumina](https://github.com/nf-core/modules/issues/3112) (ML) - PR awaiting approval - [#3114 - Pyrodigal](https://github.com/nf-core/modules/issues/3114) (@louperelo) - [#3108 - LoFreq3](https://github.com/nf-core/modules/issues/3108) (@gordondraskic, @vojislavlukic, @milenaamidzic, @alexiandra97) </small> --- # Day One - New Modules I <small> # Modules update - [#3096 - Update limma for new module standards and sample subsetting](https://github.com/nf-core/modules/issues/3096) ([Jonathan Manning](https://github.com/pinin4fjords)) - [Monday, 15:00 BST] - Work complete, ready for review in PR [#3116] - [#922 - Prodigal: zip output](https://github.com/nf-core/modules/issues/922) (@kedhammar) - PR: [#3136](https://github.com/nf-core/modules/pull/3136/) made, waiting on CI-testing and/or review - [#3143 - Allow decompression utilities to output files channels](https://github.com/nf-core/modules/issues/3143) - [Monday, 19:20 BST] - Work complete, ready for review in PR [#3144] </small> --- # Day Two - New Modules <small> - [#3157 - FastQ-Screen ](https://github.com/nf-core/modules/issues/3157)(@JPejovicApis,@snesic): - hackaton: create a module where the inputs are fastqs and config (which a user would provide), adding test files - future: discuss how to generate config file - [#2571 - MitoHiFi/findMitoReference](https://github.com/nf-core/modules/issues/2571) (@verku) cont. - in review [#3135](https://github.com/nf-core/modules/pull/3135) - [#3161 - Openvariant/groupby](https://github.com/nf-core/modules/issues/3161) (@FedericaBrando) - [#3107 - svaba](https://github.com/nf-core/modules/issues/3107) (@kubranarci) - in review - [#3173 - gridss generatebedpe](https://github.com/nf-core/modules/issues/3173) (@kubranarci) - [#3167 - gridss gridss_somatic_filter](https://github.com/nf-core/modules/issues/3167) (@kubranarci) - [#2571 - MitoHiFi/mitohifi](https://github.com/nf-core/modules/issues/2571) (@verku) - did not reach stage to create PR - [#3165 - Pyrodigal](https://github.com/nf-core/modules/pull/3165) ✅ merged (@louperelo) - [#214 - Auto rename fastq files for cellranger input](https://github.com/nf-core/scrnaseq/issues/214) (@sandrocarollo) - [#3158 - epigen](https://github.com/nf-core/modules/issues/3158) (@msantorsola) - [#3142 - IslandPath](https://github.com/nf-core/modules/issues/3142) (@jvfe) - Ready for review in PR [#3145](https://github.com/nf-core/modules/pull/3145) - [#3147 - plink/indeppairwise](https://github.com/nf-core/modules/issues/3147) (@atrigila) - Ready for review in PR [#3150] - [#1851 - picard/collectinsertsizemetrics](https://github.com/nf-core/modules/issues/1851) (@FerriolCalvet) - Ready for review in PR [[#3179](https://https://github.com/nf-core/modules/pull/3179)] - [#1792 - windowmasker/convert](https://github.com/nf-core/modules/issues/1792) (@alxndrdiaz) - Ready for review in PR [[#3152](https://github.com/nf-core/modules/pull/3152)] </small> --- # Day Two - Module updates <small> - [#922 - Prodigal: zip output](https://github.com/nf-core/modules/issues/922) (@kedhammar) - Merged - [#3115 - update RGI to latest version 6.0.2](https://github.com/nf-core/modules/issues/3115)(@louperelo) - Discussion and change of strategy on [#3143 - Allow decompression utilities to output files channels](https://github.com/nf-core/modules/issues/3143) - [Tuesday, 14:40 BST] - Re-worked to separate modules, ready for review in PR [[#3144]](https://github.com/nf-core/modules/pull/3144) - [thermorawfileparser test data PR](https://github.com/nf-core/test-datasets/pull/826) (@rolivella) - [#2957 - modifying .nf and .yml files for brackenbuild, testing locally] (@darko-cucin) - [#2572 - HyPo](https://github.com/nf-core/modules/issues/2572) (@remiolsen) - PR made: [#3181](https://github.com/nf-core/modules/pull/3181) - [#3103 - interproscan](https://github.com/nf-core/modules/issues/3103) (@toniher) - PR made: [#3180](https://github.com/nf-core/modules/pull/3180) - [#3161 - Openvariant/groupby](https://github.com/nf-core/modules/issues/3161) (@FedericaBrando) - Testing locally - [#3183 - panaroo/run](https://github.com/nf-core/modules/issues/3183) (@jvfe) - PR made: [#3184](https://github.com/nf-core/modules/pull/3184) </small> --- # Day Two - Imaging Intro 1/6 <img src="https://raw.githubusercontent.com/labsyspharm/mcmicro/master/docs/assets/images/mcmicro-logo.svg" width="40%" height="30%"></img> - Multiplexed tissue imaging techniques such as CyCIF, CODEX and MIBI require several processing steps including registration, segmentation and quantification to provide cell-by-feature arays for dowstream analysis - MCMICRO is a nextflow pipeline for this, enabling scalable and robust analysis! - Teams at HMS, U Heidleberg and Sage Bionetworks are working add key tools as nf-core modules with a view to adding as a nf-core module (next hackathon goals!) - Join us in the #mcmicro channel or at mcmicro.org --- # Day Two - Imaging 2/6 <small></small> #### 🚀🎉 Merged! - 💜 [#2864 - Ilastik/pixelclassification](https://github.com/nf-core/modules/issues/2864) (FW) - 💜 [#2865 - Ilastik/Multicut](https://github.com/nf-core/modules/issues/2865) (FW) </small> --- # Day Two - Imaging 3/6 <small> - 🧡 [#3090 - Background subtraction](https://github.com/nf-core/modules/issues/3090) (KB) - [Tuesday AM] Working from nf-core but test dataset does not have the right metadata. - Small tonsil test data should work - Need to add columns channels (to open PR) - 🧡 [#3089 - BaSicPy](https://github.com/nf-core/modules/issues/3089) (VP) - 🧡 [#3076 - Deepcell/Mesmer](https://github.com/nf-core/modules/issues/3076) (CS, MI) - [Tuesday AM] Need image with membrane and nuclear marker (can use tonsil test data (waiting on registered image)) </small> --- # Day Two - Imaging 4/6 <small> - 🧡 [#3065 - Coreograph](https://github.com/nf-core/modules/issues/3065) (AH, MC) - [Tuesday AM] Agree to skip bioconda for now with exceptions in test file,. Submit just DAPI from exemplar-001 as test-dataset - 🧡 [#3044 - Mcquant](https://github.com/nf-core/modules/issues/3044) (FW) - [Tuesday AM] Passing. Can test once test data is merged </small> --- # Day Two - Imaging 5/6 <small> - 🧡 [#2477 - Mindagap/Mindagap](https://github.com/nf-core/modules/issues/2477) (FW) - [Tuesday AM] Refactoring to add mandatory args - 🧡 [#3095 - SpatialData/MCMICRO](https://github.com/nf-core/modules/issues/3095) (WM, FW) - [Tues AM] Creating CLI tool. Looking for valid output directories. - 💚 [#3100 - Scimap/MCMICRO](https://github.com/nf-core/modules/issues/3100) (LKC, DM, CS) - [Tues AM] Have positive results with test data. wiring parameters together. Used quantification test data. </small> --- # Day Two - Imaging test data 6/6 Preparing end-to-end test datasets for imaging modules - [Rename spatialomics to imaging for modules branch #815](https://github.com/nf-core/test-datasets/issues/815) - [Add test datasets for imaging modules](https://github.com/nf-core/test-datasets/issues/822) - BASIC, exemplar-001 downsized, with downsized illumination profiles (VP) - ASHLAR (PR open, AT/JM) - Backsub (Uses registered image) - Choreograph (DAPI only from -001) - Registered image (AT) - Labelled tiff mask output - Quantification - csv --- # Day Three Wrap Up: Modules --- # Day Three Imaging Summary <small> - 🔬+🍏=❤️ Thank you for welcoming microscopists and image analysts into the nf-core community! - 🚀 11 modules started with several merged/lined up. We almost have a draft workflow for multiplexed image processing using all `nf-core` modules in place - 🤩 Huge shout out to Florian Wuennemann, Adam Taylor, Margot Chazotte, Jose Nimo, Chiara Schiller, Kresimir Bestak, Aroj Hada, Wouter-Michiel Vierdag, Luis Kuhn Cuellar, Carolin Schwitalla, Dominik Molitor, Jelena Pejovic Simeunovic, Svetozar Nesic and Emilio Palumbo - 🥳 Thank you for those who have reviewed and advised us! - 🔬 Join #microscopy on nf-core slack to bring more imaging to `nf-core` (MCMICRO+Janelia+EMBL-EBI+ more!) </small> --- ## Imaging Agenda for Weds AM <small> - Todays goals - Remaining modules into PR and merge (expect slow down in tests) - Demo pipeline (from samplesheet, or revert to single file) <div class="multi-column"> <div> <br> - Blockers - BaSiCPy - Docker container not working for nextflow - Python script is parsing args in a odd way - Reqriting to use argparse. - This is for BaSicPy - Module status - Wrap up - Issues - Project board - HackMD - Planning Spreadsheet </div> <div> <br> - Follow up on Slack - #microscopy (nf-core) - #mcmicro (HMS) - #mcmicro (nf-core) - Follow up on MCMICRO community call - Hackathon debrief - GalaxyME - Follow up on Bioconda environments - 🎉 </div> </div> </small> --- # Imaging Module Status <small> - 💚💜 [#3044 - Mcquant](https://github.com/nf-core/modules/issues/3044) (FW) - [Wednesday AM] Passing tests with public test-datasets. PR opened: https://github.com/nf-core/modules/pull/3196 - [Wednesday 2:17 CET] Auto-merge enabled. - 💚💜 [#2477 - Mindagap/Mindagap](https://github.com/nf-core/modules/issues/2477) (FW) - [Wednesday AM] Passing tests with public test-datasets. PR opened: https://github.com/nf-core/modules/pull/2860 - [Wednesday 2:17 CET] Auto-merge enabled. - 🧡 [#3095 - SpatialData/MCMICRO](https://github.com/nf-core/modules/issues/3095) (WM) - Figuring out options for storage, and compression - Documentation for docker image completed - Push to docker container - Start module </small> --- <small> - 💚 [#3100 - Scimap/MCMICRO](https://github.com/nf-core/modules/issues/3100) (LKC, DM, CS) - Streamlined some of the process, working but now testing with new test data - 🧡 [#3065 - Coreograph](https://github.com/nf-core/modules/issues/3065) (AH, MC) - Minor issue with versions being worked on - PR nearly ready - ~~AT to add test dataset once shared -sent on slack - 💚 [#3090 - Background subtraction](https://github.com/nf-core/modules/issues/3090) (KB) - Previous issues fixed. Adding test-datasets - Fixed versioning - passing Docker and Singularity tests with public dataset - PR ready for review </small> --- <small> - 🧡 [#3089 - BaSicPy](https://github.com/nf-core/modules/issues/3089) (VP) - See blockers above - 🧡 [#3076 - Deepcell/Mesmer](https://github.com/nf-core/modules/issues/3076) (CS, MI) - Failed for singularity - Adding new test datasets - 🧡 [#3111 cellpose](https://github.com/nf-core/modules/issues/3111) - Passes initial tests, to add PR and test datasets </small> --- Merged! <small> - 💜 [#2864 - Ilastik/pixelclassification](https://github.com/nf-core/modules/issues/2864) (FW) - 💜 [#2865 - Ilastik/Multicut](https://github.com/nf-core/modules/issues/2865) (FW) - [Test nf-core pipeline repo](https://github.com/SchapiroLabor/mcmicro_nfcore_demo) (FW) </small> --- # Day Three - New Modules <small> - [#3193 - bamstats](https://github.com/nf-core/modules/issues/3193) (@emi80) - [#3106 - Cellranger-arc modules](https://github.com/nf-core/modules/pull/3106)(@heylf) - [#2450 - Cellranger-atac modules](https://github.com/nf-core/modules/pull/2450)(@heylf) - [#3202 - vg/pack](https://github.com/nf-core/modules/pull/3202)(@WackerO) In progress, trying to get a GAM file for test runs - [#2807 - svtyper](https://github.com/nf-core/modules/issues/2807) (@kubranarci) </small> --- <small> - [#3213 - rgi/autoload](https://github.com/nf-core/modules/issues/3213) (@louperelo) - [#3122 - WisecondorX/newref](https://github.com/nf-core/modules/issues/3122) (@nvnieuwk) - [#3108 - LoFreq3](https://github.com/nf-core/modules/issues/3108) (@gordondraskic) - [#3109 - chopper](https://github.com/nf-core/modules/issues/3109) - [Pull Request](https://github.com/nf-core/modules/pull/3174) was reviewed, and is waiting for last round of checks to finish before merge </small> --- # Day Three - Module updates <small> - [#3143 - Allow decompression utilities to output files channels](https://github.com/nf-core/modules/issues/3143) - [Tuesday, 12:00 BST] - Complete and merged [[#3144]](https://github.com/nf-core/modules/pull/3144) - [#3115 - update RGI to latest version 6.0.2](https://github.com/nf-core/modules/issues/3115)(@louperelo) - depends on #3213 new module: rgi/autoload - [#3193 - new module: bamstats](https://github.com/nf-core/modules/issues/3193) - module and tests completed - containers URLs depend on [bioconda #40108](https://github.com/bioconda/bioconda-recipes/pull/40108) </small> --- <small> - [#3157 - FastQ-Screen ](https://github.com/nf-core/modules/issues/3157)(@JPejovicApis,@snesic) continue work on a new module - [#2572 - HyPo](https://github.com/nf-core/modules/issues/2572) (@remiolsen) - PR under review: [#3181](https://github.com/nf-core/modules/pull/3181) - [#3103 - interproscan](https://github.com/nf-core/modules/issues/3103) (@toniher) - PR under review: [#3180](https://github.com/nf-core/modules/pull/3180) - [#3108 - LoFreq3](https://github.com/nf-core/modules/tree/3108-new-module-lofreq3/modules/nf-core/lofreq3) (@gordondraskic, @VojislavLukic) - processes for lofreq variant calling and pileup added - dockerfile created and tested - meta.yml files updated </small> --- <small> - [#3218 - thermorawfileparser](https://github.com/nf-core/modules/pull/3218) (@rolivella) - Adding extra input params. - Adding a stub. - Adding test_data.config - [#3127 - purecn/intervalfile](https://github.com/nf-core/modules/pull/3127) (@lbeltrame, @aldosr) - Module set up and tested locally - Waiting for (https://github.com/bioconda/bioconda-recipes/pull/40076) to finish testing </small> --- <small> - [#3132 - purecn/coverage](https://github.com/nf-core/modules/pull/3132) (@lbeltrame, @aldosr) - Module set up - Waiting (https://github.com/bioconda/bioconda-recipes/pull/40076) for testing - [#3129 - purecn/normaldb](https://github.com/nf-core/modules/pull/3129) (@lbeltrame, @aldosr) - Module set up - Waiting (https://github.com/bioconda/bioconda-recipes/pull/40076) for testing - [#3140 - purecn/run](https://github.com/nf-core/modules/pull/3140) (@lbeltrame, @aldosr) - Module set up - Waiting (https://github.com/bioconda/bioconda-recipes/pull/40076) for testing - [#2957] - testing brackenbuild locally with docker and preparing for the commiting and pushing (@darko-cucin) - [#3217 - pyrodigal](https://github.com/nf-core/modules/issues/3217) Compress pyrodigal output files (@jasmezz) - [# 3206 - sambamba_sort](https://github.com/nf-core/modules/pull/3206) sorting bam file using sambamba sort (@BioInf2305) - implementing changes after review </small> --- # 🥳 # Thank you to all who have worked on modules! --- <style> body { background-image: url(https://raw.githubusercontent.com/nf-core/logos/master/nf-core-logos/nf-core-logo-square.svg); background-size: 7.5%; background-repeat: no-repeat; background-position: 3% 96%; background-color: #181a1b; } .reveal body { font-family: 'Roboto', sans-serif; font-weight: 300; color: white; } .reveal p { font-family: 'Roboto', sans-serif; font-weight: 300; color: white; } .reveal h1 { font-family: 'Roboto', sans-serif; font-style: bold; font-weight: 400; color: white; font-size: 62px; } .reveal h2 { font-family: 'Roboto', sans-serif; font-weight: 300; color: white; } .reveal h3 { font-family: 'Roboto', sans-serif; font-style: italic; font-weight: 300; color: white; } .reveal p { font-family: 'Roboto', sans-serif; font-weight: 300; color: white; } .reveal li { font-family: 'Roboto', sans-serif; font-weight: 300; color: white; } .reveal pre { background-color: #272822 !important; display: inline-block; border-radius: 7px; color: #aaaba9; } .reveal pre code { color: #eeeeee; background-color: #272822; font-size: 100%; } .reveal code { background-color: #272822; font-size: 75%; } .reveal .progress { color: #24B064; } .reveal .controls button { color: #24B064; } .reveal blockquote { display: block; position: relative; width: 90%; margin: 20px auto; padding: 5px; background: rgba(255, 255, 255, 0.05); box-shadow: 0px 0px 2px rgb(0 0 0 / 20%); } .multi-column{ display: grid; grid-auto-flow: column; } </style>

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