nf-core
      • Sharing URL Link copied
      • /edit
      • View mode
        • Edit mode
        • View mode
        • Book mode
        • Slide mode
        Edit mode View mode Book mode Slide mode
      • Customize slides
      • Note Permission
      • Read
        • Owners
        • Signed-in users
        • Everyone
        Owners Signed-in users Everyone
      • Write
        • Owners
        • Signed-in users
        • Everyone
        Owners Signed-in users Everyone
      • Engagement control Commenting, Suggest edit, Emoji Reply
      • Invitee
    • Publish Note

      Share your work with the world Congratulations! 🎉 Your note is out in the world Publish Note

      Your note will be visible on your profile and discoverable by anyone.
      Your note is now live.
      This note is visible on your profile and discoverable online.
      Everyone on the web can find and read all notes of this public team.
      See published notes
      Unpublish note
      Please check the box to agree to the Community Guidelines.
      View profile
    • Commenting
      Permission
      Disabled Forbidden Owners Signed-in users Everyone
    • Enable
    • Permission
      • Forbidden
      • Owners
      • Signed-in users
      • Everyone
    • Suggest edit
      Permission
      Disabled Forbidden Owners Signed-in users Everyone
    • Enable
    • Permission
      • Forbidden
      • Owners
      • Signed-in users
    • Emoji Reply
    • Enable
    • Versions and GitHub Sync
    • Note settings
    • Engagement control
    • Transfer ownership
    • Delete this note
    • Insert from template
    • Import from
      • Dropbox
      • Google Drive
      • Gist
      • Clipboard
    • Export to
      • Dropbox
      • Google Drive
      • Gist
    • Download
      • Markdown
      • HTML
      • Raw HTML
Menu Note settings Sharing URL Help
Menu
Options
Versions and GitHub Sync Engagement control Transfer ownership Delete this note
Import from
Dropbox Google Drive Gist Clipboard
Export to
Dropbox Google Drive Gist
Download
Markdown HTML Raw HTML
Back
Sharing URL Link copied
/edit
View mode
  • Edit mode
  • View mode
  • Book mode
  • Slide mode
Edit mode View mode Book mode Slide mode
Customize slides
Note Permission
Read
Owners
  • Owners
  • Signed-in users
  • Everyone
Owners Signed-in users Everyone
Write
Owners
  • Owners
  • Signed-in users
  • Everyone
Owners Signed-in users Everyone
Engagement control Commenting, Suggest edit, Emoji Reply
Invitee
Publish Note

Share your work with the world Congratulations! 🎉 Your note is out in the world Publish Note

Your note will be visible on your profile and discoverable by anyone.
Your note is now live.
This note is visible on your profile and discoverable online.
Everyone on the web can find and read all notes of this public team.
See published notes
Unpublish note
Please check the box to agree to the Community Guidelines.
View profile
Engagement control
Commenting
Permission
Disabled Forbidden Owners Signed-in users Everyone
Enable
Permission
  • Forbidden
  • Owners
  • Signed-in users
  • Everyone
Suggest edit
Permission
Disabled Forbidden Owners Signed-in users Everyone
Enable
Permission
  • Forbidden
  • Owners
  • Signed-in users
Emoji Reply
Enable
Import from Dropbox Google Drive Gist Clipboard
   owned this note    owned this note      
Published Linked with GitHub
Subscribed
  • Any changes
    Be notified of any changes
  • Mention me
    Be notified of mention me
  • Unsubscribe
Subscribe
--- title: Modules Team - nf-core Hackathon March 2023 tags: march-2023,nf-core,hackathon,progress description: View the slide with "Slide Mode". --- <!-- .slide: data-background="https://raw.githubusercontent.com/maxulysse/maxulysse.github.io/main/assets/img/svg/green_white_bg.svg" --> <a href="https://www.nf-co.re"><img src="https://i.imgur.com/hEDjpCs.png" width="65%"><img></a> ### 🚀 Modules Team 🚀 _27th-29th March 2023_ The Hackathon March 2023 [Project Board](https://github.com/orgs/nf-core/projects/38) The Group Discussion Channel `#hackathon-march-2023-modules` <img src="https://openmoji.org/data/color/svg/E040.svg" width=50> @nf-core / <img src="https://openmoji.org/data/color/svg/E045.svg" width=50> @nf-core --- # Modules Team --- # Team Leads - General advice, pointers, co-ordination etc. - Maxime Garcia (@maxulysse) - Matthias De Smet (@matthdsm) - Harshil Patel (@drpatelh) - Jonathan Manning (@pinin4fjords) - Nicolas Vannieuwkerke (@nvnieuwk) --- - Highly-multiplexed imaging subgroup - Adam Taylor (@adamjtaylor) - Florian Wuennemann (@FloWuenne) _But always ask first in: `#hackathon-march-2023-modules`!_ --- # Tasks Ideas - General development work - New modules - Fix modules tests - Improve modules --- - Highly-multiplexed imaging / Spatial Omics - Add new modules geared at this type of data analsysis, starting with porting core modules of [MCMICRO](https://mcmicro.org/) - Please label issues `imaging` in Github - [Imaging specific project board view](https://github.com/orgs/nf-core/projects/38/views/7?filterQuery=repo%3A%22nf-core%2Fmodules%22+label%3A%22new+module%22++label%3Aimaging) --- # Remember - Add yourself to Team Members slide - Assign yourself on issues on the [project board](https://github.com/orgs/nf-core/projects/38) - Post your progress here **daily**! - Sign up for a reviewing buddy (see slide)! --- # Team Members <div class="multi-column"> <div> - [Maxime Garcia](@maxulysse) - [Verena Kutschera](@verku) - [Adrien Coulier](@Aratz) - [Bojan Balic](@boleodole) - [Darko Cucin](@darko-cucin) - [Remi-Andre Olsen](@remiolsen) - [Sandro Carollo](@sandrocarollo) </div> <div> - [Georgios Fotakis](@abyssum) - [Valentin Marteau](@vjmarteau) - [Marie Lataretu](@MarieLataretu) - [Fynn Freyer](@FynnFreyer) - [Toni Hermoso Pulido](@toniher) - [Paul Wolk](@paulwolk) - [Roger Olivella](@rolivellla) - [Maulik Upadhyay](@BioInf2305) </div> </div> --- <div class="multi-column"> <div> - [Lili Andersson-Li](@Lili) - [Joan Gaztelu](@LlaneroHiboreo) - [Narci](@kubranarci) - [Aleksandra Vitkovac](@alexiandra97) - [Anabella Trigila](@atrigila) - [Mariangela Santorsola](@msantorsola) - [Florian Heyl](@heylf) </div> <div> - [Milena Amidzic](@milenaamidzic) - [Gordon Draskic](@gordondraskic) - [Vojislav Lukic](@VojislavLukic) - [Alfred Kedhammar](@kedhammar) - [Louisa Perelo](@louperelo) - [Ferriol Calvet](@ferriolcalvet) - [Federica Brando](@FedericaBrando) </div> </div> --- ## Highly-multiplexed imaging subgroup <div class="multi-column"> <div> - [Florian Wuennemann](@FloWuenne) - [Adam Taylor](@adamjtaylor) - [Margot Chazotte](@MargotCh) - [Jose Nimo](@josenimo) - [Chiara Schiller](@chiarasch) - [Kresimir Bestak](@kbestak) </div> <div> - [Aroj Hada](@ArozHada) - [Miguel A. Ibarra-Arellano](@migueLib) - [Victor Perez](@VictorDidier) - [Wouter-Michiel Vierdag](@melonora) - [Luis Kuhn Cuellar](@luiskuhn) - [Carolin Schwitalla ](@CaroAMN) - [Dominik Molitor](@DojakM) - [Jelena Pejovic Simeunovic](@JPejovicApis) - [Svetozar Nesic](@snesic) - [Emilio Palumbo](@emi80) </div> </div> --- # Reviewing Buddy List <div class="multi-column"> <div> | Person A | Person B | |-------------|----------| | Bojan | Darko | | Kresimir | Chiara | | Aroj | Margot | | Jose | Florian | | Gordon | Vojislav | | Vojislav | Gordon | </div> <div> | Person A | Person B | |-------------|----------| | Fynn | Paul | | Marie | Fynn | | Paul | Marie | | Verena | Alfred | | Louisa | Jasmin | </div> </div> --- # Day One - Imaging I/IV <small> - 🧡 [#3090 - Background subtraction](https://github.com/nf-core/modules/issues/3090) (KB) - [Monday, 3.30 CET] Assigned and in progress but no linked branch/PR CET - [EOD] figuring out passing optional inputs such as px size - [Tuesday AM] Working from nf-core but test dataset does not have the right metadata. - Small tonsil test data should work - Need to add columns to it - 🧡 [#3089 - BaSicPy](https://github.com/nf-core/modules/issues/3089) (VP) - [Monday, 3.30 CET] Assigned and in progress but no linked branch/PR CET - [EOD] looking at passing optional inputs. Issues with docker container working - [Tuesday AM] - 🧡 [#3076 - Deepcell/Mesmer](https://github.com/nf-core/modules/issues/3076) (CS, MI) - [Monday, 3.30 CET] Assigned and in progress but no linked branch/PR CET - [EOD] Passes testing with local data - Detail work for optional inputs ongoing - [Tuesday AM] Need image with membrane and nuclear marker (does this work? https://github.com/nf-core/test-datasets/pull/821) </small> --- # Day One - Imaging II/IV <small> - 🧡 [#3065 - Coreograph](https://github.com/nf-core/modules/issues/3065) (AH, MC) - [Monday, 3.30 CET] Assigned and in progress but no linked branch/PR CET - [EOD] Need to make bioconda package. Asking for help on this. (Really nice to have bioconda even if not strictly required) - To make seperate HackMD for technical details at this stage. Can skip bioconda tests manually - [Tuesday AM] Agree to skip bioconda for now with exceptions in test file,. Submit just DAPI from exemplar-001 as test-dataset - 🧡 [#3044 - Mcquant](https://github.com/nf-core/modules/issues/3044) (FW) - [Monday, 3.30 CET] Assigned and in progress but no linked branch/PR CET - Waiting on a suitable test-dataset from AT - [EOD] As above. Works on real data! - [Tuesday AM] Can test once test data is merged </small> --- # Day One - Imaging III/IV <small> - 💜 [#2864 - Ilastik/pixelclassification](https://github.com/nf-core/modules/issues/2864) (FW) - [Monday, 3.30 CET] Assigned and ready for review - PR [#2875](https://github.com/nf-core/modules/pull/2875) approved by Nicolas Vannieuwkerke - 16.10 CET Merged! - [EOD] Conda tests skipped as has its own coda channel - 💜 [#2865 - Ilastik/Multicut](https://github.com/nf-core/modules/issues/2865) (FW) - [Monday, 3.30 CET] Assigned and ready for review - PR [#2894](https://github.com/nf-core/modules/pull/2894) approved by Nicolas Vannieuwkerke, waiting for review by Maxime Garcia => Failing CI tests - [EOD] prettier lint failing. Request out on how to solve - 11.35 CET -> Merged </small> --- # Day One - Imaging IV/IV <small> - 🧡 [#2477 - Mindagap/Mindagap](https://github.com/nf-core/modules/issues/2477) (FW) - [Monday, 3.30 CET] Assigned and ready for review - PR [#2477](https://github.com/nf-core/modules/issues/2477) - Three reviewers assigned - Fails nf-core linting - [EOD] stub test needed - [Tuesday AM] Refactoring to add mandatory args - 🧡 [#3095 - SpatialData/MCMICRO](https://github.com/nf-core/modules/issues/3095) (WM, FW) - [Monday, 3.30 CET] Assigned and in progress but no linked branch/PR CET - [EOD] Docker image being built for this. Figuring out right output structure. Draft working - [Tues AM] Creating CLI tool. Looking for valid output directories. - 💚 [#3100 - Scimap/MCMICRO](https://github.com/nf-core/modules/issues/3100) (LKC, DM, CS) - [Monday, 16:00 CET] Worked on general structure - [EOD] First module attempt 🚀. Have basic structure but not working yet. Need to confirm right test data. - [Tues AM] Have positive results with test data. wiring parameters together. Used quantification test data. </small> --- # Day One - New Modules I <small> - [#2571 - MitoHiFi/findMitoReference](https://github.com/nf-core/modules/issues/2571) (@verku) - [#3103 - interproscan](https://github.com/nf-core/modules/issues/3103) (@toniher) - [#14 - RNA Velocity](https://github.com/nf-core/scrnaseq/issues/14) - [#3109 - chopper](https://github.com/nf-core/modules/issues/3109) (@FynnFreyer) - [PR](https://github.com/nf-core/modules/pull/3174) waiting for review - [#3105 - thermorawfileparser](https://github.com/nf-core/modules/issues/3105) (@rolivella) - [#3113 - SURVIVOR/FILTER](https://github.com/nf-core/modules/issues/3113) (@LlaneroHiboreo) - [#2572 - HyPo](https://github.com/nf-core/modules/issues/2572) (@remiolsen) - [#2937 - purecn/intervalfile](https://github.com/nf-core/modules/issues/2937) (@lbeltrame, @aldosr) - [#2938 - purecn/coverage](https://github.com/nf-core/modules/issues/2938) (@lbeltrame, @aldosr) - [#2939 - purecn/normaldb](https://github.com/nf-core/modules/issues/2939) (@lbeltrame, @aldosr) - [#2940 - purecn/run](https://github.com/nf-core/modules/issues/2940) (@lbeltrame, @aldosr) - [#3078 - sigprofilerextractor](https://github.com/nf-core/modules/issues/3078) (@ferriolcalvet) - [#3147 - plink/indeppairwise](https://github.com/nf-core/modules/issues/3147) (@atrigila) </small> --- # Day One - New Modules II <small> - [#2940 - purecn/dx](https://github.com/nf-core/modules/issues/2941) (@lbeltrame, @aldosr) - [#3087 - bioqc](https://github.com/nf-core/modules/issues/3087) (@boleodole) - [#1869 - wisecondorx/convert](https://github.com/nf-core/modules/issues/1869) (@nvnieuwk) - PR awaiting approval - [#3133 - sambamba/flagstat](https://github.com/nf-core/modules/issues/3133) (@kubranarci) - [#2957 - brackenbuild](https://github.com/nf-core/modules/issues/2957) (@darko-cucin) - [#3110 - NanoComp](https://github.com/nf-core/modules/issues/3110) (@paulwolk) - Monday: Getting familiar with nf-core modules and writing the base of the module - Tuesday: Testing - Wednesday: PR awaiting approval - [#3112 - ART/art_illumina](https://github.com/nf-core/modules/issues/3112) (ML) - PR awaiting approval - [#3114 - Pyrodigal](https://github.com/nf-core/modules/issues/3114) (@louperelo) - [#3108 - LoFreq3](https://github.com/nf-core/modules/issues/3108) (@gordondraskic, @vojislavlukic, @milenaamidzic, @alexiandra97) </small> --- # Day One - New Modules I <small> # Modules update - [#3096 - Update limma for new module standards and sample subsetting](https://github.com/nf-core/modules/issues/3096) ([Jonathan Manning](https://github.com/pinin4fjords)) - [Monday, 15:00 BST] - Work complete, ready for review in PR [#3116] - [#922 - Prodigal: zip output](https://github.com/nf-core/modules/issues/922) (@kedhammar) - PR: [#3136](https://github.com/nf-core/modules/pull/3136/) made, waiting on CI-testing and/or review - [#3143 - Allow decompression utilities to output files channels](https://github.com/nf-core/modules/issues/3143) - [Monday, 19:20 BST] - Work complete, ready for review in PR [#3144] </small> --- # Day Two - New Modules <small> - [#3157 - FastQ-Screen ](https://github.com/nf-core/modules/issues/3157)(@JPejovicApis,@snesic): - hackaton: create a module where the inputs are fastqs and config (which a user would provide), adding test files - future: discuss how to generate config file - [#2571 - MitoHiFi/findMitoReference](https://github.com/nf-core/modules/issues/2571) (@verku) cont. - in review [#3135](https://github.com/nf-core/modules/pull/3135) - [#3161 - Openvariant/groupby](https://github.com/nf-core/modules/issues/3161) (@FedericaBrando) - [#3107 - svaba](https://github.com/nf-core/modules/issues/3107) (@kubranarci) - in review - [#3173 - gridss generatebedpe](https://github.com/nf-core/modules/issues/3173) (@kubranarci) - [#3167 - gridss gridss_somatic_filter](https://github.com/nf-core/modules/issues/3167) (@kubranarci) - [#2571 - MitoHiFi/mitohifi](https://github.com/nf-core/modules/issues/2571) (@verku) - did not reach stage to create PR - [#3165 - Pyrodigal](https://github.com/nf-core/modules/pull/3165) ✅ merged (@louperelo) - [#214 - Auto rename fastq files for cellranger input](https://github.com/nf-core/scrnaseq/issues/214) (@sandrocarollo) - [#3158 - epigen](https://github.com/nf-core/modules/issues/3158) (@msantorsola) - [#3142 - IslandPath](https://github.com/nf-core/modules/issues/3142) (@jvfe) - Ready for review in PR [#3145](https://github.com/nf-core/modules/pull/3145) - [#3147 - plink/indeppairwise](https://github.com/nf-core/modules/issues/3147) (@atrigila) - Ready for review in PR [#3150] - [#1851 - picard/collectinsertsizemetrics](https://github.com/nf-core/modules/issues/1851) (@FerriolCalvet) - Ready for review in PR [[#3179](https://https://github.com/nf-core/modules/pull/3179)] - [#1792 - windowmasker/convert](https://github.com/nf-core/modules/issues/1792) (@alxndrdiaz) - Ready for review in PR [[#3152](https://github.com/nf-core/modules/pull/3152)] </small> --- # Day Two - Module updates <small> - [#922 - Prodigal: zip output](https://github.com/nf-core/modules/issues/922) (@kedhammar) - Merged - [#3115 - update RGI to latest version 6.0.2](https://github.com/nf-core/modules/issues/3115)(@louperelo) - Discussion and change of strategy on [#3143 - Allow decompression utilities to output files channels](https://github.com/nf-core/modules/issues/3143) - [Tuesday, 14:40 BST] - Re-worked to separate modules, ready for review in PR [[#3144]](https://github.com/nf-core/modules/pull/3144) - [thermorawfileparser test data PR](https://github.com/nf-core/test-datasets/pull/826) (@rolivella) - [#2957 - modifying .nf and .yml files for brackenbuild, testing locally] (@darko-cucin) - [#2572 - HyPo](https://github.com/nf-core/modules/issues/2572) (@remiolsen) - PR made: [#3181](https://github.com/nf-core/modules/pull/3181) - [#3103 - interproscan](https://github.com/nf-core/modules/issues/3103) (@toniher) - PR made: [#3180](https://github.com/nf-core/modules/pull/3180) - [#3161 - Openvariant/groupby](https://github.com/nf-core/modules/issues/3161) (@FedericaBrando) - Testing locally - [#3183 - panaroo/run](https://github.com/nf-core/modules/issues/3183) (@jvfe) - PR made: [#3184](https://github.com/nf-core/modules/pull/3184) </small> --- # Day Two - Imaging Intro 1/6 <img src="https://raw.githubusercontent.com/labsyspharm/mcmicro/master/docs/assets/images/mcmicro-logo.svg" width="40%" height="30%"></img> - Multiplexed tissue imaging techniques such as CyCIF, CODEX and MIBI require several processing steps including registration, segmentation and quantification to provide cell-by-feature arays for dowstream analysis - MCMICRO is a nextflow pipeline for this, enabling scalable and robust analysis! - Teams at HMS, U Heidleberg and Sage Bionetworks are working add key tools as nf-core modules with a view to adding as a nf-core module (next hackathon goals!) - Join us in the #mcmicro channel or at mcmicro.org --- # Day Two - Imaging 2/6 <small></small> #### 🚀🎉 Merged! - 💜 [#2864 - Ilastik/pixelclassification](https://github.com/nf-core/modules/issues/2864) (FW) - 💜 [#2865 - Ilastik/Multicut](https://github.com/nf-core/modules/issues/2865) (FW) </small> --- # Day Two - Imaging 3/6 <small> - 🧡 [#3090 - Background subtraction](https://github.com/nf-core/modules/issues/3090) (KB) - [Tuesday AM] Working from nf-core but test dataset does not have the right metadata. - Small tonsil test data should work - Need to add columns channels (to open PR) - 🧡 [#3089 - BaSicPy](https://github.com/nf-core/modules/issues/3089) (VP) - 🧡 [#3076 - Deepcell/Mesmer](https://github.com/nf-core/modules/issues/3076) (CS, MI) - [Tuesday AM] Need image with membrane and nuclear marker (can use tonsil test data (waiting on registered image)) </small> --- # Day Two - Imaging 4/6 <small> - 🧡 [#3065 - Coreograph](https://github.com/nf-core/modules/issues/3065) (AH, MC) - [Tuesday AM] Agree to skip bioconda for now with exceptions in test file,. Submit just DAPI from exemplar-001 as test-dataset - 🧡 [#3044 - Mcquant](https://github.com/nf-core/modules/issues/3044) (FW) - [Tuesday AM] Passing. Can test once test data is merged </small> --- # Day Two - Imaging 5/6 <small> - 🧡 [#2477 - Mindagap/Mindagap](https://github.com/nf-core/modules/issues/2477) (FW) - [Tuesday AM] Refactoring to add mandatory args - 🧡 [#3095 - SpatialData/MCMICRO](https://github.com/nf-core/modules/issues/3095) (WM, FW) - [Tues AM] Creating CLI tool. Looking for valid output directories. - 💚 [#3100 - Scimap/MCMICRO](https://github.com/nf-core/modules/issues/3100) (LKC, DM, CS) - [Tues AM] Have positive results with test data. wiring parameters together. Used quantification test data. </small> --- # Day Two - Imaging test data 6/6 Preparing end-to-end test datasets for imaging modules - [Rename spatialomics to imaging for modules branch #815](https://github.com/nf-core/test-datasets/issues/815) - [Add test datasets for imaging modules](https://github.com/nf-core/test-datasets/issues/822) - BASIC, exemplar-001 downsized, with downsized illumination profiles (VP) - ASHLAR (PR open, AT/JM) - Backsub (Uses registered image) - Choreograph (DAPI only from -001) - Registered image (AT) - Labelled tiff mask output - Quantification - csv --- # Day Three Wrap Up: Modules --- # Day Three Imaging Summary <small> - 🔬+🍏=❤️ Thank you for welcoming microscopists and image analysts into the nf-core community! - 🚀 11 modules started with several merged/lined up. We almost have a draft workflow for multiplexed image processing using all `nf-core` modules in place - 🤩 Huge shout out to Florian Wuennemann, Adam Taylor, Margot Chazotte, Jose Nimo, Chiara Schiller, Kresimir Bestak, Aroj Hada, Wouter-Michiel Vierdag, Luis Kuhn Cuellar, Carolin Schwitalla, Dominik Molitor, Jelena Pejovic Simeunovic, Svetozar Nesic and Emilio Palumbo - 🥳 Thank you for those who have reviewed and advised us! - 🔬 Join #microscopy on nf-core slack to bring more imaging to `nf-core` (MCMICRO+Janelia+EMBL-EBI+ more!) </small> --- ## Imaging Agenda for Weds AM <small> - Todays goals - Remaining modules into PR and merge (expect slow down in tests) - Demo pipeline (from samplesheet, or revert to single file) <div class="multi-column"> <div> <br> - Blockers - BaSiCPy - Docker container not working for nextflow - Python script is parsing args in a odd way - Reqriting to use argparse. - This is for BaSicPy - Module status - Wrap up - Issues - Project board - HackMD - Planning Spreadsheet </div> <div> <br> - Follow up on Slack - #microscopy (nf-core) - #mcmicro (HMS) - #mcmicro (nf-core) - Follow up on MCMICRO community call - Hackathon debrief - GalaxyME - Follow up on Bioconda environments - 🎉 </div> </div> </small> --- # Imaging Module Status <small> - 💚💜 [#3044 - Mcquant](https://github.com/nf-core/modules/issues/3044) (FW) - [Wednesday AM] Passing tests with public test-datasets. PR opened: https://github.com/nf-core/modules/pull/3196 - [Wednesday 2:17 CET] Auto-merge enabled. - 💚💜 [#2477 - Mindagap/Mindagap](https://github.com/nf-core/modules/issues/2477) (FW) - [Wednesday AM] Passing tests with public test-datasets. PR opened: https://github.com/nf-core/modules/pull/2860 - [Wednesday 2:17 CET] Auto-merge enabled. - 🧡 [#3095 - SpatialData/MCMICRO](https://github.com/nf-core/modules/issues/3095) (WM) - Figuring out options for storage, and compression - Documentation for docker image completed - Push to docker container - Start module </small> --- <small> - 💚 [#3100 - Scimap/MCMICRO](https://github.com/nf-core/modules/issues/3100) (LKC, DM, CS) - Streamlined some of the process, working but now testing with new test data - 🧡 [#3065 - Coreograph](https://github.com/nf-core/modules/issues/3065) (AH, MC) - Minor issue with versions being worked on - PR nearly ready - ~~AT to add test dataset once shared -sent on slack - 💚 [#3090 - Background subtraction](https://github.com/nf-core/modules/issues/3090) (KB) - Previous issues fixed. Adding test-datasets - Fixed versioning - passing Docker and Singularity tests with public dataset - PR ready for review </small> --- <small> - 🧡 [#3089 - BaSicPy](https://github.com/nf-core/modules/issues/3089) (VP) - See blockers above - 🧡 [#3076 - Deepcell/Mesmer](https://github.com/nf-core/modules/issues/3076) (CS, MI) - Failed for singularity - Adding new test datasets - 🧡 [#3111 cellpose](https://github.com/nf-core/modules/issues/3111) - Passes initial tests, to add PR and test datasets </small> --- Merged! <small> - 💜 [#2864 - Ilastik/pixelclassification](https://github.com/nf-core/modules/issues/2864) (FW) - 💜 [#2865 - Ilastik/Multicut](https://github.com/nf-core/modules/issues/2865) (FW) - [Test nf-core pipeline repo](https://github.com/SchapiroLabor/mcmicro_nfcore_demo) (FW) </small> --- # Day Three - New Modules <small> - [#3193 - bamstats](https://github.com/nf-core/modules/issues/3193) (@emi80) - [#3106 - Cellranger-arc modules](https://github.com/nf-core/modules/pull/3106)(@heylf) - [#2450 - Cellranger-atac modules](https://github.com/nf-core/modules/pull/2450)(@heylf) - [#3202 - vg/pack](https://github.com/nf-core/modules/pull/3202)(@WackerO) In progress, trying to get a GAM file for test runs - [#2807 - svtyper](https://github.com/nf-core/modules/issues/2807) (@kubranarci) </small> --- <small> - [#3213 - rgi/autoload](https://github.com/nf-core/modules/issues/3213) (@louperelo) - [#3122 - WisecondorX/newref](https://github.com/nf-core/modules/issues/3122) (@nvnieuwk) - [#3108 - LoFreq3](https://github.com/nf-core/modules/issues/3108) (@gordondraskic) - [#3109 - chopper](https://github.com/nf-core/modules/issues/3109) - [Pull Request](https://github.com/nf-core/modules/pull/3174) was reviewed, and is waiting for last round of checks to finish before merge </small> --- # Day Three - Module updates <small> - [#3143 - Allow decompression utilities to output files channels](https://github.com/nf-core/modules/issues/3143) - [Tuesday, 12:00 BST] - Complete and merged [[#3144]](https://github.com/nf-core/modules/pull/3144) - [#3115 - update RGI to latest version 6.0.2](https://github.com/nf-core/modules/issues/3115)(@louperelo) - depends on #3213 new module: rgi/autoload - [#3193 - new module: bamstats](https://github.com/nf-core/modules/issues/3193) - module and tests completed - containers URLs depend on [bioconda #40108](https://github.com/bioconda/bioconda-recipes/pull/40108) </small> --- <small> - [#3157 - FastQ-Screen ](https://github.com/nf-core/modules/issues/3157)(@JPejovicApis,@snesic) continue work on a new module - [#2572 - HyPo](https://github.com/nf-core/modules/issues/2572) (@remiolsen) - PR under review: [#3181](https://github.com/nf-core/modules/pull/3181) - [#3103 - interproscan](https://github.com/nf-core/modules/issues/3103) (@toniher) - PR under review: [#3180](https://github.com/nf-core/modules/pull/3180) - [#3108 - LoFreq3](https://github.com/nf-core/modules/tree/3108-new-module-lofreq3/modules/nf-core/lofreq3) (@gordondraskic, @VojislavLukic) - processes for lofreq variant calling and pileup added - dockerfile created and tested - meta.yml files updated </small> --- <small> - [#3218 - thermorawfileparser](https://github.com/nf-core/modules/pull/3218) (@rolivella) - Adding extra input params. - Adding a stub. - Adding test_data.config - [#3127 - purecn/intervalfile](https://github.com/nf-core/modules/pull/3127) (@lbeltrame, @aldosr) - Module set up and tested locally - Waiting for (https://github.com/bioconda/bioconda-recipes/pull/40076) to finish testing </small> --- <small> - [#3132 - purecn/coverage](https://github.com/nf-core/modules/pull/3132) (@lbeltrame, @aldosr) - Module set up - Waiting (https://github.com/bioconda/bioconda-recipes/pull/40076) for testing - [#3129 - purecn/normaldb](https://github.com/nf-core/modules/pull/3129) (@lbeltrame, @aldosr) - Module set up - Waiting (https://github.com/bioconda/bioconda-recipes/pull/40076) for testing - [#3140 - purecn/run](https://github.com/nf-core/modules/pull/3140) (@lbeltrame, @aldosr) - Module set up - Waiting (https://github.com/bioconda/bioconda-recipes/pull/40076) for testing - [#2957] - testing brackenbuild locally with docker and preparing for the commiting and pushing (@darko-cucin) - [#3217 - pyrodigal](https://github.com/nf-core/modules/issues/3217) Compress pyrodigal output files (@jasmezz) - [# 3206 - sambamba_sort](https://github.com/nf-core/modules/pull/3206) sorting bam file using sambamba sort (@BioInf2305) - implementing changes after review </small> --- # 🥳 # Thank you to all who have worked on modules! --- <style> body { background-image: url(https://raw.githubusercontent.com/nf-core/logos/master/nf-core-logos/nf-core-logo-square.svg); background-size: 7.5%; background-repeat: no-repeat; background-position: 3% 96%; background-color: #181a1b; } .reveal body { font-family: 'Roboto', sans-serif; font-weight: 300; color: white; } .reveal p { font-family: 'Roboto', sans-serif; font-weight: 300; color: white; } .reveal h1 { font-family: 'Roboto', sans-serif; font-style: bold; font-weight: 400; color: white; font-size: 62px; } .reveal h2 { font-family: 'Roboto', sans-serif; font-weight: 300; color: white; } .reveal h3 { font-family: 'Roboto', sans-serif; font-style: italic; font-weight: 300; color: white; } .reveal p { font-family: 'Roboto', sans-serif; font-weight: 300; color: white; } .reveal li { font-family: 'Roboto', sans-serif; font-weight: 300; color: white; } .reveal pre { background-color: #272822 !important; display: inline-block; border-radius: 7px; color: #aaaba9; } .reveal pre code { color: #eeeeee; background-color: #272822; font-size: 100%; } .reveal code { background-color: #272822; font-size: 75%; } .reveal .progress { color: #24B064; } .reveal .controls button { color: #24B064; } .reveal blockquote { display: block; position: relative; width: 90%; margin: 20px auto; padding: 5px; background: rgba(255, 255, 255, 0.05); box-shadow: 0px 0px 2px rgb(0 0 0 / 20%); } .multi-column{ display: grid; grid-auto-flow: column; } </style>

Import from clipboard

Paste your markdown or webpage here...

Advanced permission required

Your current role can only read. Ask the system administrator to acquire write and comment permission.

This team is disabled

Sorry, this team is disabled. You can't edit this note.

This note is locked

Sorry, only owner can edit this note.

Reach the limit

Sorry, you've reached the max length this note can be.
Please reduce the content or divide it to more notes, thank you!

Import from Gist

Import from Snippet

or

Export to Snippet

Are you sure?

Do you really want to delete this note?
All users will lose their connection.

Create a note from template

Create a note from template

Oops...
This template has been removed or transferred.
Upgrade
All
  • All
  • Team
No template.

Create a template

Upgrade

Delete template

Do you really want to delete this template?
Turn this template into a regular note and keep its content, versions, and comments.

This page need refresh

You have an incompatible client version.
Refresh to update.
New version available!
See releases notes here
Refresh to enjoy new features.
Your user state has changed.
Refresh to load new user state.

Sign in

Forgot password

or

By clicking below, you agree to our terms of service.

Sign in via Facebook Sign in via Twitter Sign in via GitHub Sign in via Dropbox Sign in with Wallet
Wallet ( )
Connect another wallet

New to HackMD? Sign up

Help

  • English
  • 中文
  • Français
  • Deutsch
  • 日本語
  • Español
  • Català
  • Ελληνικά
  • Português
  • italiano
  • Türkçe
  • Русский
  • Nederlands
  • hrvatski jezik
  • język polski
  • Українська
  • हिन्दी
  • svenska
  • Esperanto
  • dansk

Documents

Help & Tutorial

How to use Book mode

Slide Example

API Docs

Edit in VSCode

Install browser extension

Contacts

Feedback

Discord

Send us email

Resources

Releases

Pricing

Blog

Policy

Terms

Privacy

Cheatsheet

Syntax Example Reference
# Header Header 基本排版
- Unordered List
  • Unordered List
1. Ordered List
  1. Ordered List
- [ ] Todo List
  • Todo List
> Blockquote
Blockquote
**Bold font** Bold font
*Italics font* Italics font
~~Strikethrough~~ Strikethrough
19^th^ 19th
H~2~O H2O
++Inserted text++ Inserted text
==Marked text== Marked text
[link text](https:// "title") Link
![image alt](https:// "title") Image
`Code` Code 在筆記中貼入程式碼
```javascript
var i = 0;
```
var i = 0;
:smile: :smile: Emoji list
{%youtube youtube_id %} Externals
$L^aT_eX$ LaTeX
:::info
This is a alert area.
:::

This is a alert area.

Versions and GitHub Sync
Get Full History Access

  • Edit version name
  • Delete

revision author avatar     named on  

More Less

Note content is identical to the latest version.
Compare
    Choose a version
    No search result
    Version not found
Sign in to link this note to GitHub
Learn more
This note is not linked with GitHub
 

Feedback

Submission failed, please try again

Thanks for your support.

On a scale of 0-10, how likely is it that you would recommend HackMD to your friends, family or business associates?

Please give us some advice and help us improve HackMD.

 

Thanks for your feedback

Remove version name

Do you want to remove this version name and description?

Transfer ownership

Transfer to
    Warning: is a public team. If you transfer note to this team, everyone on the web can find and read this note.

      Link with GitHub

      Please authorize HackMD on GitHub
      • Please sign in to GitHub and install the HackMD app on your GitHub repo.
      • HackMD links with GitHub through a GitHub App. You can choose which repo to install our App.
      Learn more  Sign in to GitHub

      Push the note to GitHub Push to GitHub Pull a file from GitHub

        Authorize again
       

      Choose which file to push to

      Select repo
      Refresh Authorize more repos
      Select branch
      Select file
      Select branch
      Choose version(s) to push
      • Save a new version and push
      • Choose from existing versions
      Include title and tags
      Available push count

      Pull from GitHub

       
      File from GitHub
      File from HackMD

      GitHub Link Settings

      File linked

      Linked by
      File path
      Last synced branch
      Available push count

      Danger Zone

      Unlink
      You will no longer receive notification when GitHub file changes after unlink.

      Syncing

      Push failed

      Push successfully