msleeper

@msleeper

Joined on Aug 21, 2023

  • Student: Meghan Sleeper Project: Identifying commonly differentially methylated regions (DMRs) in colorectal cancer tissue samples Committee members: Gordon Wolfe, David Keller, Carter Tilquist Updates :::success Since our last committee meeting, I have converted my pipeline into an automated workflow using Snakemake, added quality control steps to my pipeline, and organized sample metadata into a uable format accessed by the pipeline. :::
     Like  Bookmark
  • Student: Meghan Sleeper :::info Student: Meghan Sleeper Project: Identifying commonly differentially methylated regions (DMRs) in colorectal cancer tissue samples ::: :beginner: Updates :::success
     Like  Bookmark
  • :::info Student: Meghan Sleeper Project: Identifying commonly differentially methylated regions (DMRs) in colorectal cancer tissue samples ::: :triangular_flag_on_post: Project Background :::success My project seeks to aggregate genome wide methylation data for colorectal cancer patients and healthy patients and identify common differentially methylated regions across all samples. :::
     Like  Bookmark
  • Author: Meghan Sleeper Class: MATH 615 1. Topic area statement: DNA has always fascinated me because it is essentially the code for living organisms. DNA sequences hold the recipes for proteins, which can be manufactured via the molecular processes of transcription and translation (collectively known as gene expression). It is DNA and these tiny molecular processes that make life possible. Interestingly, nearly all cells in the human body contain the same DNA sequence and yet, each cell type has unique characteristics that allow for specialized functions. Modifications on top of DNA (epigenetic modifications) allow for regulation of these characteristics by activating or repressing gene expression. DNA methylation is one form of epigentic modification. It is generally accepted that high levels of methylation (hypermethylation) is associated with gene repression, while low levels of methylation (hypomethylation) allow for gene expression. In recent research, studies have found that changes to DNA methylation may play a role in the development of diseases such as cancer. By comparing DNA methylation in healthy patients and cancer patients we can identify regions of DNA that are differentially methylated (referred to as differentially methylated regions or DMRs). These DMRs can tell us a story about the molecular processes potentially impacted in a cancer.
     Like  Bookmark