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# System prepended metadata

title: NHGRI Short Course 2022 - DNA Barcoding

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# NHGRI Short Course 2022 - DNA Barcoding

**Contact Information** 

Jason Williams
- (e) williams@cshl.edu
- (t) @JasonWilliamsNY 

## Resources 

- [DNA Subway](https://dnasubway.cyverse.org/)
    - [DNA Subway Barcoding manual](https://learning.cyverse.org/dna_subway_guide/#walkthrough-of-dna-subway-blue-line-dna-barcoding-and-phylogenetics)
- [CyVerse User Portal](https://user.cyverse.org/)
- [DNA Barcoding 101](https://dnabarcoding101.org/)
    - [Barcoding protocls](https://dnabarcoding101.org/lab/)
    - [Barcoding programs](https://dnabarcoding101.org/programs/)
    - [Student projects](https://dnabarcoding101.org/projects/)
- [DNA Subway Mosquito lesson](https://qubeshub.org/publications/165/serve/2?render=archive)
- [DNALC Live Barcoding Wetlab playlist](https://dnalc.cshl.edu/resources/dnalc-live/series-dna-barcoding.html)
- [DNALC Live Barcoding Bioinformatics playlist](https://dnalc.cshl.edu/resources/dnalc-live/series-barcoding-bioinformatics.html)


## Workshop notes 
Ana Munoz: I have used this website to buy kits for CRISPR (worked very well), and they also have some cheap lab supplies: https://www.the-odin.com/
I have extracted bacteria DNA from soil with the "ready to go kits" 
    We currently use minipreps but am hoping for something cheaper for the larger classes.
Ron: Are there consortia of schools outside of NYC that share resources?
Adam Reeves: Gram negative organisms can be lysed with any lysis buffer, but Gram positive organisms require treatment with lysozyme(https://www.thermofisher.com/order/catalog/product/89833) for at least an hour before proceeding with extraction. This website is a cheap way to extract DNA using home solutions - https://sfvideo.blob.core.windows.net/sitefinity/docs/default-source/biotech-basics/at-home-strawberry-dna-extraction.pdf?sfvrsn=62563407_4#:~:text=For%20each%20100%20ml%20of,until%20the%20salt%20is%20dissolved.&text=Other%2C%20very%20good%2C%20protocols%20can,the%20web%20sites%20provided%20below.



