---
# System prepended metadata

title: SampleSheets

---

# SampleSheets

### Novaseq
Som det ser ut just nu:
```
[Header],,,,,,,,,,
IEMFileVersion,5,,,,,,,,,
Investigator Name,SH,,,,,,,,,
Experiment Name,WGS_Prep54_Pool54_201127_SH,,,,,,,,,
Date,2020-11-27,,,,,,,,,
Workflow,GenerateFASTQ,,,,,,,,,
Application,NovaSeq FASTQ Only,,,,,,,,,
Instrument Type,NovaSeq,,,,,,,,,
Assay,TruSeq DNA PCR-Free,,,,,,,,,
Chemistry,Amplicon,,,,,,,,,
,,,,,,,,,,
[Reads],,,,,,,,,,
151,,,,,,,,,,
151,,,,,,,,,,
,,,,,,,,,,
[Settings],,,,,,,,,,
Adapter,AGATCGGAAGAGCACACGTCTGAACTCCAGTCA,,,,,,,,,
AdapterRead2,AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT,,,,,,,,,
,,,,,,,,,,
[Data],,,,,,,,,,
Sample_ID,Sample_Name,Sample_Plate,Sample_Well,Index_Plate_Well,I7_Index_ID,index,I5_Index_ID,index2,Sample_Project,Description
DNA70860,DNA70860_201127_AHGGVGDSXY,,,A01,UDI0001,CCGCGGTT,UDI0001,AGCGCTAG,KG,CGG_M_00_i_70860_
DNA71107,DNA71107_201127_AHGGVGDSXY,,,B01,UDI0002,TTATAACC,UDI0002,GATATCGA,KG,CGG_M_00_far_70860_
DNA71108,DNA71108_201127_AHGGVGDSXY,,,C01,UDI0003,GGACTTGG,UDI0003,CGCAGACG,KG,CGG_F_00_mor_70860_
```

#### Beslut:
Fortsätter som är just nu

### Nextseq
Som det ser ut nu:

```
[Header],,,,,,,,,,
IEMFileVersion,5,,,,,,,,,
Investigator Name,AS-GS,,,,,,,,,
Experiment Name,Twist-hematolg-panel,,,,,,,,,
Date,8/20/2020,,,,,,,,,
Workflow,GenerateFASTQ,,,,,,,,,
Application,NextSeq FASTQ Only,,,,,,,,,
Instrument Type,NextSeq/MiniSeq,,,,,,,,,
Assay,TruSeq Nano DNA,,,,,,,,,
Index Adapters,TruSeq DNA CD Indexes (96 Indexes),,,,,,,,,
Chemistry,Amplicon,,,,,,,,,
,,,,,,,,,,
[Reads],,,,,,,,,,
151,,,,,,,,,,
151,,,,,,,,,,
,,,,,,,,,,
[Settings],,,,,,,,,,
Adapter,AGATCGGAAGAGCACACGTCTGAACTCCAGTCA,,,,,,,,,
AdapterRead2,AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT,,,,,,,,,
Read1UMILength,3,,,,,,,,,
Read2UMILength,3,,,,,,,,,
Read1StartFromCycle,6,,,,,,,,,
Read2StartFromCycle,6,,,,,,,,,
,,,,,,,,,,
[Data],,,,,,,,,,
Sample_ID,Sample_Name,Sample_Plate,Sample_Well,Index_Plate_Well,I7_Index_ID,index,I5_Index_ID,index2,Sample_Project,Description
T1,T1,,,A04,i7-1,CCTTGATC,i5-1,GATGGAGT,kk,gms-myeloid_tp53
T2,T2,,,B04,i7-2,GTCGAAGA,i5-2,CCACATTG,kk,gms-myeloid_all
T3,T3,,,C04,i7-3,ACCACGAT,i5-3,GTCTGCAA,kk,gms-myeloid_all
T4,T4,,,D04,i7-4,GATTACCG,i5-4,TTGGACTG,kk,gms-myeloid_all
T5,T5,,,E04,i7-5,GCACAACT,i5-5,CTGAACGT,kk,gms-myeloid_tp53
T6,T6,,,F04,i7-6,GCGTCATT,i5-6,CAGACGTT,kk,gms-myeloid_all
T7,T7,,,G04,i7-7,ATCCGGTA,i5-7,GACCGATA,kk,gms-myeloid_all
HD829,HD829,,,H04,i7-8,CGTTGCAA,i5-8,ATAGAGCG,kk,gms-myeloid_all
N1,N1,,,A05,i7-9,GTGAAGTG,i5-9,GAGCAATC,kk,gms-myeloid_all
N2,N2,,,B05,i7-10,CATGGCTA,i5-10,CACACATC,kk,gms-myeloid_all
N3,N3,,,C05,i7-11,ATGCCTGT,i5-11,AGATTGCG,kk,gms-myeloid_all
N4,N4,,,D05,i7-12,CAACACCT,i5-12,AGCTACCA,kk,gms-myeloid_all
N5,N5,,,E05,i7-13,TGTGACTG,i5-13,AGCCTATC,kk,gms-myeloid_all
N6,N6,,,F05,i7-14,GTCATCGA,i5-14,GATCCACT,kk,gms-myeloid_all
N7,N7,,,G05,i715,AGCACTTC,i5-15,ACGTCCAA,kk,gms-myeloid_all
```

#### Beslut
* Sample_Project=KK får endast användas av de prover som ska in i SLIMS
* I description fältet ska "gms_myeloid_all" eller "gms_myelooid_tp53" anges. Om det är tomt blir det "all" som default.
* Giti och Anna Staffas behöver den informationen
* Använd unika namn för Sample_ID/Sample_Name

### Miseq
Som det ser ut nu:

```
[Header]
IEMFileVersion,4
Investigator Name,GA
Experiment Name,myeloidpanel
Date,2020-11-25
Workflow,Custom Amplicon
Application,TruSeq Amplicon
Assay,TruSeq Amplicon
Description,201125pool
Chemistry,Amplicon

[Manifests]
A,TruSight-Myeloid-Manifest.txt

[Reads]
201
201

[Settings]
VariantCaller,Somatic
VariantFilterQualityCutoff,30
outputgenomevcf,TRUE

[Data]
Sample_ID,Sample_Name,Sample_Plate,Sample_Well,I7_Index_ID,index,I5_Index_ID,index2,Manifest,GenomeFolder,Sample_Project,Description
620200890,,,,A707,CCCAACCT,A501,TGAACCTT,A,Homo_sapiens\UCSC\hg19\Sequence\WholeGenomeFasta,,
620200907,,,,A707,CCCAACCT,A502,TGCTAAGT,A,Homo_sapiens\UCSC\hg19\Sequence\WholeGenomeFasta,,
```

NPM1:
```
[Header],,,,,,,,,
IEMFileVersion,4,,,,,,,,
Investigator Name,GS,,,,,,,,
Experiment Name,UDS_NPM1_BM_glas_flowcell39_4nM,,,,,,,,
Date,10/6/2020,,,,,,,,
Workflow,GenerateFASTQ,,,,,,,,
Application,FASTQ Only,,,,,,,,
Assay,TruSeq HT,,,,,,,,
Description,NPM1,,,,,,,,
Chemistry,Amplicon,,,,,,,,
,,,,,,,,,
[Reads],,,,,,,,,
151,,,,,,,,,
151,,,,,,,,,
,,,,,,,,,
[Settings],,,,,,,,,
,,,,,,,,,
[Data],,,,,,,,,
Sample_ID,Sample_Name,Sample_Plate,Sample_Well,I7_Index_ID,index,I5_Index_ID,index2,Sample_Project,Description
UDS_NPM1_55773034_,UDS_NPM1_55773034_,,,A701,ATCACGAC,A501,TGAACCTT,UDS_NPM1_BM_glas_F39,MRD_AML
UDS_NOPHO_Normal_TSPAN15,UDS_NOPHO_Normal_TSPAN15,,,A702,ACAGTGGT,A502,TGCTAAGT,UDS_NPM1_BM_glas_F39,MRD_AML
UDS_NPM1_55773055,UDS_NPM1_55773055,,,A703,CAGATCCA,A503,TGTTCTCT,UDS_NPM1_BM_glas_F39,MRD_AML
UDS_NPM1_55773079,UDS_NPM1_55773079,,,A704,ACAAACGG,A504,TAAGACAC,UDS_NPM1_BM_glas_F39,MRD_AML
UDS_NPM1_55773080,UDS_NPM1_55773080,,,A705,ACCCAGCA,A505,CTAATCGA,UDS_NPM1_BM_glas_F39,MRD_AML
UDS_NPM1_BM_glas_25016028,UDS_NPM1_BM_glas_25016028,,,A708,CACCACAC,A506,CTAGAACA,UDS_NPM1_BM_glas_F39,MRD_AML
UDS_NPM1_BM_glas_25016052,UDS_NPM1_BM_glas_25016052,,,A709,GAAACCCA,A507,TAAGTTCC,UDS_NPM1_BM_glas_F39,MRD_AML
UDS_NPM1_BM_glas_25160464wt,UDS_NPM1_BM_glas_25160464wt,,,A710,TGTGACCA,A508,TAGACCTA,UDS_NPM1_BM_glas_F39,MRD_AML
```

#### Beslut:
Vi fortsätter som vanligt