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+ coûts exome ?
  + murin, devis chez GeneWiz a 402 € pour du 10gb par échantillon (120x).
  + humain, Exome Agilent 13,5 Gb 2x150bp, lot de 48 échantillons: 499€/échantillon chez Eurofins (150x)



+ coût IGH/TCR protocole Chudakov
  + primers 
  + Sequencing en 400+100nt (quel séquenceur pour nous en pratique ?)
     + 30% PhiX
     + samples in replicates !
     + 6 samples (3x2 samples) per miseq run
     + miSeq en 600nt : 1509 € HT

  + Sample Prep
      + A partir de l'article _(CRITICAL The materials listed here are those needed for the example)_
        + Blood collection tubes (e.g., BD Plastic Whole Blood tube with spray-coatedK 2 EDTA, cat. no. 367844)
        + Ficoll Paque (GE Healthcare, cat. no. 17-1440-02)
        + PBS (Sigma-Aldrich, cat. no. D8537)
        + Trizol Reagent (Invitrogen, cat. no. 15596-026) or Qiazol Lysis Reagent (Qiagen, cat. no. 79306) 
        + Chloroform (Sigma-Aldrich, cat. no. C2432-25ML)
        + Isopropyl alcohol (Sigma-Aldrich, cat. no. I9516-25ML) 
        + Co-precipitant (e.g., Merck Millipore Pellet Paint, cat. no. 69049)
        + RNAse-free water
        + Oligonucleotides (e.g., Evrogen JSC, see Table 2 for sequences) _ CRITICAL The 5′-template-switch adaptor (SmartNNNa) must be HPLC-purified._
        + SMARTscribe reverse transcriptase (Clontech, cat. no. 639536) or Mint reverse transcriptase (Evrogen, cat. no. SK005)  CRITICAL Reverse transcriptase with high template switching activity 57 must be used.
        + dNTPs (Life Technologies, cat. no. R0181)
        + RNAse inhibitor (Promega, cat. no. N2111)
        + Uracil DNA glycosylase (New England Biolabs, cat. no. M0280S)
        + Q5 Hot-start High-Fidelity DNA Polymerase (New England Biolabs     cat. no. M0493S)
        + QIAquick PCR Purification Kit (Qiagen, cat. no. 28104)
        + MinElute PCR Purification Kit (Qiagen, cat. no. 28004)
        + TAE buffer (Sigma-Aldrich, cat. no. T8280)
        + Agarose (Sigma-Aldrich, cat. no. A0169)
        + Ethidium bromide (Invitrogen, cat. no. 15585011) or any alternative nucleic acid gel stain (e.g., SYBR Gold, Invitrogen, cat. no. S11494)
        + 1-kB DNA ladder (Sybenzyme, cat. no. M11)
        + 100-bp DNA ladder (Sybenzyme, cat. no. M15)
        + NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs, cat. no. E6040)
        + NEBNext Multiplex Oligos for Illumina (Index Primers Sets 1 and 2, cat. no. E7335, E7500)
        + Agencourt AMPure XP (Beckman Coulter, cat. no. A63881)
        + 96% (vol/vol) ethanol (Sigma-Aldrich, cat. no. E7148) 
        + RNAeasy Micro Kit (Qiagen, cat. no. 74004)
    + Non listés ?
        + RNA 6000
        + HS chips