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ImageJ 教學：分析海水表面溫度

作者：王一哲
第1版：2016/4/6
第2版：2020/6/11

安裝所需軟體

Java Runtime Environment

請到此頁面選擇配合作業系統的 JRE (Java Runtime Enviroment)，目前最新的版本為 Version 8 Update 251，分為下幾種作業系統

  1. Windows 32-bit
  2. Windows 64-bit
  3. Linux 32-bit
  4. Linux 64-bit
Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
https://www.java.com/zh_TW/download/manual.jsp

Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →

Windows 64-bit 版安裝畫面



Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →

Windows 64-bit 版安裝成功畫面


安裝 Windows 版 ImageJ

請到此頁面下載檔案，目前最新的版本為 1.5.3， 此軟體不需要安裝，只要將下載後的檔案解壓縮，執行 ImageJ 資料夾當中的 ImageJ.exe 即可。

Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
ImageJ 官方網站

Linux 系統上的安裝方法

我測試的環境為 Lubuntu 19.04，只要開啟終端機輸入並執行以下指令即可

sudo apt install imagej

若使用其它版本的 Linux，請參考這個網頁。

利用色階校正分析全球海水表面平均溫度圖

下載要分析的圖片

由美國國家海洋暨大氣總署（National Oceanic and Atmospheric Administration, NOAA）網站找到所需圖片並複製圖片連結。網頁下方有不同日期、位置的圖片可以選擇。


Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
https://www.ospo.noaa.gov/Products/ocean/sst/50km_night/index.html

將所選的圖片匯入 ImageJ

  1. 我選用的圖片為2016年4月21日全球夜間海水表面溫度圖，圖片連結為 https://www.ospo.noaa.gov/data/sst/50km_night/2016/sstnight.4.21.2016.gif
  2. 將圖片匯入 ImageJ，依序點選 File → Import → URL → 貼上連結後按OK。

Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
https://www.ospo.noaa.gov/data/sst/50km_night/2016/sstnight.4.21.2016.gif

Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
File → Import → URL

Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
輸入超連結視窗

檢驗圖例中色階的變化是否為線性

  1. 利用主視窗裡的直線工具，在圖片最下方的圖例中拉一條直線。
  2. 由主視窗的工具列依序點選 Analyze → Plot Profile 或按下 Ctrl+K 畫出直線上灰階值與位置的關係圖。
  3. 圖形為斜直線，代表圖例中的色階為線性變化。

Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
ImageJ 主視窗

Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
在下方的圖例中畫直線

Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
圖例中的色階灰階值與位置的關係

將圖例部分放大

  1. 利用主視窗裡的矩形工具選取圖例的最左側
  2. 將畫面放大到所選取的部分，依序點選 Image → Zoom → To Selection

Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
Image → Zoom → To Selection

測量圖例中每個溫度標示對應的灰階值

  1. 利用主視窗裡的直線工具，在圖例中最左側有標示數值的位置畫直線。
  2. 由主視窗的工具列依序點選 Analyze → Measure 或按下 Ctrl+M 測量直線所在位置的灰階值。
  3. 重複以上步驟，測量圖例中所有標示數值位置對應的灰階值。

Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
在圖例中有標示數值的位置畫直線

Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
每個有標示數值處對應的灰階值

將灰階值換算成溫度

  1. 由主視窗的工具列依序點選 Analyze → Calibrate
  2. Calibrate 視窗左側為剛才測量的數值，右側要自行填入對應的溫度，以本例而言為：-2.0 0.0 2.0 4.0 6.0 8.0 10.0 12.0 14.0 16.0 18.0 20.0 22.0 24.0 26.0 28.0 30.0 32.0 34.0
  3. 選擇函數為 Straight Line ，單位改為 C ，按下 OK 後，會顯示換算的圖形及公式。
  4. 回到原來的圖片，當滑鼠滑標移到圖上時，主視窗下的狀態列會顯示此處對應的溫度。

Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
Calibrate 視窗

Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
線性擬合的結果

Image Not Showing Possible Reasons
  • The image file may be corrupted
  • The server hosting the image is unavailable
  • The image path is incorrect
  • The image format is not supported
Learn More →
校正後的圖片及讀取結果

參考資料

用imagej作grayscale的校正 測量海溫圖上的溫度資訊。2016年4月25日。取自 https://www.youtube.com/watch?v=y4l3BBQK428&list=PLm6x13NbsKl5So8XGnb4RgvLhrQCVzvnX&index=7 。


tags:ImageJ
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