# Edis - Myoglobin

# UNIT 1
## Aufgabe 1
BIG PICTURE 1: According to reports sperm whale Mb was the **first** three-dimensional structure obtained of any protein (by x-ray diffraction).

BIG PICTURE 2: In 2050, science will succeed in transferring the oxygen affinity of myoglobin to other proteins through genetic modification.

BIG PICTURE 3: Scientific knowledge about myoglobin has made the link between smoking and the risk of heart attack much more understandable.

BIG PICTURE 4: Nothing in life is more difficult than giving up a thing/habit that you have had for a long time. This is how my smoking habit became my undoing. You should take care of your health while you have it and not when it is already too late.

# UNIT 2+3
### Aufgabe 1
Complete molecular biology of the gene. Database: Ensemble.org

- NG_007075 RefSeqGene and only ATG as start-codon


### Aufgabe 2

- NM_203377.1 and only ATG as start-codon


- The higher the orf length is set, the fewer search results are found, as the smaller orf sequences are excluded.

### Aufgabe 3

### Aufgabe 4

### Aufgabe 5

- Comparison of the MB genome with other species -> highest similarity with Pan panicus (pygmy chimpanzee) -> was expected


- Similarity at 99% !


##### - Following graphics showing the comparison of the MB genome and the genome with inserted mutations (50 mutations into each sequence; https://www.bioinformatics.org/sms2/mutate_dna.html)



- For the inserted mutations, there were changes in the match around sequence regions 8,000 and 16,000 of the genome sequence. Introns 1-2 and 2-3 are located in this region, so if mutations actually occurred in these regions, there would be no effect on the protein, as only the exons are important for the mRNA. The introns are cut out anyway by splicer enzymes. However, other mutations, for example those that are also found in the exon region, could have an effect on the translation of the mRNA into the protein, since these carry the information about how the protein should later look and what function(s) it should have. These mutations in the exons could therefore lead to a defective function of the protein or it could fail completely and have no function at all.