# 台灣人體微生物相資料平台 - NCBI SRA Submission Portal

###### tags: `人體微生物相資料平台教學`
## 上傳SRA數據至NCBI整個流程大綱

## 上傳SRA數據至NCBI網址
https://submit.ncbi.nlm.nih.gov/subs/

## 第一部分 BioProject submission
### BioProject流程大綱

### BioProject網址


### Step1: Submitter

### Step2: Project Type

#### Project Type
> A general label indicating the primary study goal.
> 1. Genome sequencing and assembly
> 2. Raw sequence reads
> 3. Genome sequencing
> 4. Assembly
> 5. Clone ends
> 6. Epigenomics
> 7. Exome
> 8. Map
> 9. Metagenome
> 10. Metagenomic assembly
> 11. Phenotype or Genotype
> 12. Proteome
> 13. Random survey
> 14. Targeted loci cultured
> 15. Targeted loci environmental
> 16. Targeted Locus (Loci)
> 17. Transcriptome or Gene expression
> 18. Variation
> 19. Other

#### Sample scope
> Indicates the scope and purity of the biological sample used for the study.
> Choose Multiisolate as the scope when the goal of the research is to compare multiple individuals or strains of the same species, eg, in a "Variation" or "Genome sequencing and assembly" project.
> Choose Multispecies when different species are being examined.
> Choose Monoisolate if the goal is to make a single genome or transcriptome assembly, even if more than one individual was the source of the DNA or RNA.
>
> 1. Monoisolate: a single animal, cultured cell-line, inbred population (or possibly a heterogeneous population when a single genome assemby is generated from the pooled sample; not preferred).
> 2. Multiisolate: multiple individuals, a population (representative of a species). To be used for variation or other sequence comparison projects, not when multiple genomes will be annotated. Make separate monoisolate projects when more than one genome will be annotated.
> 3. Multi-species: sample represents multiple species.
> 4. Environment: the species content of the sample is not known.
> 5. Synthetic: the sample is synthetically created by a machine.
> 6. Other: specify the sample scope that was used.
>

### Step 3: Target

### Step 4: General Info

### Step 5: BioSample

### Step 6: Publications

### Step 7: Review & Submit

### Step 8: Result

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## 第二部分 BioSample submission
### BioProject流程大綱

### BioProject網址


### Step1: Submitter

### Step2: General Information

### Step3: Sample Type

### Step4: ATTRIBUTES
https://submit.ncbi.nlm.nih.gov/biosample/template/


### Step 7: Review & Submit

### Step 8: Result

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## 第三部分 SRA submission
### SRA 流程大綱

### SRA 網址

### Step1: Submitter

## Step2: General Information

## Step3: SRA metadata


## Step4: Files




### Step 5: Result

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## 第四部分 ASPERA submission
### 至NCBI取得 ASPERA 上傳網址

### 至 q2.hmp.org.tw 上傳數據
1. 複製Examples Disk內的 9-ncbi-SRA-submit資料夾 至 Local Disk, 並將您要上傳的FASTQ檔案上傳至input資料夾

2. 點選網址 q2.hmp.org.tw 上面Plugin下拉選單內的SRA Submit, 進而點選綠色UNDO按鈕

3. 進行FASTQ上傳至NCBI作業
* 確認上傳的FASTQ數量
* 填寫上傳的ASPERA路徑 https://submit.ncbi.nlm.nih.gov/subs/sra/
* 填寫上傳儲存的目錄