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台灣人體微生物相資料平台 - NCBI SRA Submission Portal

tags: 人體微生物相資料平台教學

上傳SRA數據至NCBI整個流程大綱

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上傳SRA數據至NCBI網址

https://submit.ncbi.nlm.nih.gov/subs/

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第一部分 BioProject submission

BioProject流程大綱

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BioProject網址

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Step1: Submitter

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Step2: Project Type

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Project Type

A general label indicating the primary study goal.

  1. Genome sequencing and assembly
  2. Raw sequence reads
  3. Genome sequencing
  4. Assembly
  5. Clone ends
  6. Epigenomics
  7. Exome
  8. Map
  9. Metagenome
  10. Metagenomic assembly
  11. Phenotype or Genotype
  12. Proteome
  13. Random survey
  14. Targeted loci cultured
  15. Targeted loci environmental
  16. Targeted Locus (Loci)
  17. Transcriptome or Gene expression
  18. Variation
  19. Other

Sample scope

Indicates the scope and purity of the biological sample used for the study.
Choose Multiisolate as the scope when the goal of the research is to compare multiple individuals or strains of the same species, eg, in a "Variation" or "Genome sequencing and assembly" project.
Choose Multispecies when different species are being examined.
Choose Monoisolate if the goal is to make a single genome or transcriptome assembly, even if more than one individual was the source of the DNA or RNA.

  1. Monoisolate: a single animal, cultured cell-line, inbred population (or possibly a heterogeneous population when a single genome assemby is generated from the pooled sample; not preferred).
  2. Multiisolate: multiple individuals, a population (representative of a species). To be used for variation or other sequence comparison projects, not when multiple genomes will be annotated. Make separate monoisolate projects when more than one genome will be annotated.
  3. Multi-species: sample represents multiple species.
  4. Environment: the species content of the sample is not known.
  5. Synthetic: the sample is synthetically created by a machine.
  6. Other: specify the sample scope that was used.

Step 3: Target

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Step 4: General Info

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Step 5: BioSample

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Step 6: Publications

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Step 7: Review & Submit

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Step 8: Result

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第二部分 BioSample submission

BioProject流程大綱

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BioProject網址

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Step1: Submitter

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Step2: General Information

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Step3: Sample Type

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Step4: ATTRIBUTES

https://submit.ncbi.nlm.nih.gov/biosample/template/

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Step 7: Review & Submit

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Step 8: Result

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第三部分 SRA submission

SRA 流程大綱

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SRA 網址

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Step1: Submitter

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Step2: General Information

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Step3: SRA metadata

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Step4: Files

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Step 5: Result

第四部分 ASPERA submission

至NCBI取得 ASPERA 上傳網址

至 q2.hmp.org.tw 上傳數據

  1. 複製Examples Disk內的 9-ncbi-SRA-submit資料夾 至 Local Disk, 並將您要上傳的FASTQ檔案上傳至input資料夾

  2. 點選網址 q2.hmp.org.tw 上面Plugin下拉選單內的SRA Submit, 進而點選綠色UNDO按鈕

  3. 進行FASTQ上傳至NCBI作業

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