# 1. Launch EC2 Instance

* **From the EC2 console, click on `Launch Instance`**


* **For AMI, search for `amzn2-ami-kernel-5.10-hvm-2.0.20230628.0-x86_64-gp2`**


* **For Instance type, choose `t2.medium` and provide key-pair**


* **For `Network Setting` select existing `default` security-group**


* **For `Configure Storage`, 8Gb of root volume is sufficient. Proceed to click on `Launch Instance`**


# 2. SSH to Nextflow Host

* **From the EC2 console, get the public ip-address of the instance**


* **Navigate to the directory with your key-pair and change permission for the pem file to read-only**

chmod 400 sateesh_peri.pem

* **Connect to the instance**


* **Open a terminal and SSH to the nextflow host from the directory with your key-pair pem file**

ssh -i "sateesh_peri.pem" [e-mail]


# 3. Create Nextflow work directory S3 bucket

* **Navigate to S3 console & click on `Create Bucket`. Provide a name and choose the region for the bucket**


* **Uncheck the `Block all public access` box and acknowledge in the checkbox**


* **Leave defaults for the rest and click on `Create Bucket`**


### Retrieve security credentials for S3 bucket

* **Navigate to IAM console and select the user to retrieve `security credentials` and click on `Create Access Key`**

* **Choose the use case of Command Line Interface (CLI), check the confirmation and click `Next`**


* **Download the access key in csv format and save in secure location**


# 4. Install MMC float CLI tool

wget https://<op_center_ip_address>/float --no-check-certificate
sudo mv float /usr/local/bin/
sudo chmod +x /usr/local/bin/float

>example
>```bash=
>wget https://34.205.19.185/float --no-check-certificate
>sudo mv float /usr/local/bin/
>sudo chmod +x /usr/local/bin/float
>```

* **Connect float on Nextflow Host to the OpCenter (needs public ip address) with credentials below:**

**username -> `admin`
password -> `memverge`**


# 5. Install Java & Nextflow

* **install Java using [sdkman](https://sdkman.io/)**

curl -s "https://get.sdkman.io" | bash
source "/home/ec2-user/.sdkman/bin/sdkman-init.sh"

* **Install Java `17.0.6`**

sdk install java 17.0.6-tem
java -version


* **Install Nextflow**

curl -s https://get.nextflow.io | bash
sudo mv nextflow /usr/local/bin/

# 6. Install git & tmux

* **Install git**

sudo yum install git
sudo yum install tmux

---