---
# System prepended metadata

title: BOND maintenance
tags: [protocols, maintenance, bond]

---

---
title: BOND maintenance
disqus: hackmd
---
:::success
> Documentation [name=MrDr.Staffan]
###### tags: `protocols` `bond` `maintenance`

### Table of Contents
[TOC]
:::
[Top](#Table-of-Contents)



# General Maintenance
[Top](#Table-of-Contents)

Click some images for large version/video

[![](https://i.imgur.com/3Yh27Nx.jpg =350x)](https://i.imgur.com/3Yh27Nx.jpg) [![](https://i.imgur.com/2LE9IYj.jpg =350x)](https://i.imgur.com/2LE9IYj.jpg)

[![](https://i.imgur.com/WGtIOIP.jpg =350x)](https://i.imgur.com/WGtIOIP.jpg) [![](https://i.imgur.com/OAUAIE0.jpg =350x)](https://i.imgur.com/OAUAIE0.jpg)



# Empty waste & Refill bulk container 
[Top](#Table-of-Contents)

![](https://i.imgur.com/QyrStXS.jpg =350x)
One waste full and ddH~2~O running low

![](https://i.imgur.com/YGqJJ7n.jpg =350x)
Filling ddH~2~O

![](https://i.imgur.com/DoAUbKv.jpg =350x)
Removing the full waste
[![](https://i.imgur.com/HKqW5j0.png)](https://www.dropbox.com/s/jwc4cjod9zzf6br/VID_20210225_153238.mp4?dl=0)
Pour waste into container and add vircon. 1 scoop for 5l.


![](https://i.imgur.com/OagoyWb.jpg =350x)
All containers Good


# Cleaning the slide bays
[Top](#Table-of-Contents)

![](https://i.imgur.com/a0PtJYs.jpg =350x)
Open BOND lid and remove slide trays

![](https://i.imgur.com/UDFP6xb.jpg =350x)
Push and turn counter clockwise!

![](https://i.imgur.com/s8QUcwH.jpg =350x)
Open the top casette holding the slides down


**Insert time lapse Clean holder**

![](https://i.imgur.com/qPUdUSR.jpg =350x)
Pulling the metal bobs located at the ends will release the top casette



![](https://i.imgur.com/s45iFmY.jpg =350x)
Top casette removed!

**Spray**
[![](https://i.imgur.com/kUqZ10i.png =350x)](https://www.dropbox.com/s/im9rkvdf5i4ubs2/VID_20210225_152332.mp4?dl=0)

[![](https://i.imgur.com/ZxvtiXE.png)](https://www.dropbox.com/s/yofn26ttiy0wu1z/VID_20210225_152521.mp4?dl=0)
Spray down with ethanol, brush with toothbrush and wipe away all residue.

Reassemble








#  Cleaning the aspiration probe
[Top](#Table-of-Contents)






![](https://i.imgur.com/xsqNU9s.jpg =350x)
Wipe down asipirating probe


## Preparations
[Top](#Table-of-Contents)

![](https://i.imgur.com/X0eLfKk.jpg =350x)



![](https://i.imgur.com/Ml34oXW.jpg =350x)
Reagents & rack
![](https://i.imgur.com/rKzkpCi.jpg =350x)
Scanning reagents

## Run the cleaning
[Top](#Table-of-Contents)

Entering maintenance tab
![](https://i.imgur.com/qCrTVTr.jpg =350x)
![](https://i.imgur.com/pw2WHTd.jpg =350x)
Click clean aspirating


## Installing a new aspirating probe
[Top](#Table-of-Contents)

![](https://i.imgur.com/7eFj4o6.jpg =350x)


