---
title: BOND maintenance
disqus: hackmd
---
:::success
> Documentation [name=MrDr.Staffan]
###### tags: `protocols` `bond` `maintenance`

### Table of Contents
[TOC]
:::
[Top](#Table-of-Contents)

# General Maintenance
[Top](#Table-of-Contents)

Click some images for large version/video

[](https://i.imgur.com/3Yh27Nx.jpg) [](https://i.imgur.com/2LE9IYj.jpg)

[](https://i.imgur.com/WGtIOIP.jpg) [](https://i.imgur.com/OAUAIE0.jpg)

# Empty waste & Refill bulk container
[Top](#Table-of-Contents)

One waste full and ddH~2~O running low

Filling ddH~2~O

Removing the full waste
[](https://www.dropbox.com/s/jwc4cjod9zzf6br/VID_20210225_153238.mp4?dl=0)
Pour waste into container and add vircon. 1 scoop for 5l.

All containers Good

# Cleaning the slide bays
[Top](#Table-of-Contents)

Open BOND lid and remove slide trays

Push and turn counter clockwise!

Open the top casette holding the slides down

**Insert time lapse Clean holder**

Pulling the metal bobs located at the ends will release the top casette

Top casette removed!

**Spray**
[](https://www.dropbox.com/s/im9rkvdf5i4ubs2/VID_20210225_152332.mp4?dl=0)

[](https://www.dropbox.com/s/yofn26ttiy0wu1z/VID_20210225_152521.mp4?dl=0)
Spray down with ethanol, brush with toothbrush and wipe away all residue.

Reassemble

# Cleaning the aspiration probe
[Top](#Table-of-Contents)

Wipe down asipirating probe

## Preparations
[Top](#Table-of-Contents)


Reagents & rack
Scanning reagents

## Run the cleaning
[Top](#Table-of-Contents)

Entering maintenance tab
Click clean aspirating

## Installing a new aspirating probe
[Top](#Table-of-Contents)