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A book about Cell Culture
===
###### tags: `book` `cell_culture`
- [Front page](/Jlgqm1kKRI-u2d6o49Toug)
- [Note](#Book)
- [<span class="red">!</span>]()
- [<span class="green">Sheet </span>](https://docs.google.com/spreadsheets/u/0/)
- [<span class="blue">Doc </span>](https://docs.google.com/document/u/0/)
- [<span class="yellow">Slide</span>](https://docs.google.com/presentation/u/0/)
General (*permission required)
---
- [Antibodies Rowitch Masterlist LVL3 + LVL8](https://docs.google.com/spreadsheets/d/1o0aabMR6CFHilzv00JuuAB6mWLw1xRi0L9L2PhbUSMk/edit?usp=sharing)
- [Antibody lists Various](/168DPc_ERea4YFekruQVcg)
- [Co-Culture](/FUQdXo2WQYSaIzgzueBOSg)
- [Electrophys](/Q3DDUQKvTc-2SKpQQQzFvQ)
- [Electronics](/_tlxSnZoRQabPAL_pO599A)
- [Atlas](/shBclJEFRo-oJjgC4MWt3A)
- [Imaging](/yodmh4VkS6eE-ZT5TShnxw)
- [TC Core JCBC](/6iV4PbugTO6gVMVMwRx2cA)
- [Cell entrence and transduction](/fFw_Dj0OS2GvDEk1IsjHJQ)
- [Reagents, Compounds, Inhibitors](https://docs.google.com/spreadsheets/d/10e16IkGVGYTxh0exVJsc9q6Zaggp0YUaV1iSiHhVyAA/edit?usp=sharing)
- [Media per Cell type](https://docs.google.com/spreadsheets/d/1cDCVoNckS9Q4BE5QfcHXtzVzvVrbpJIE0K3s4qLFup8/edit?usp=sharing )
- [Media & Supplements](https://docs.google.com/spreadsheets/d/1mGYQChUpuz9TZ__YwbKrNexBmjytiyWWk8acNST5jMU/edit?usp=sharing)
- [Media patents](https://docs.google.com/spreadsheets/d/1_vQs770KUxd0j6AsNlp3zUAEbwrTU02RrpwyvyDRssw/edit?usp=sharing)
- [Neural differentiation](/Yxg38ZvTThesJUTj_vyHkw)
- [Cheat sheet. Areas and density](https://docs.google.com/spreadsheets/d/1NkWtMG4E1ga88kLCU6KtlkOgwzBX__Jux6BZ2Sse0-0/edit#gid=1953788813)
- [Coating](/Rh1nleFjSj60gWdCqtRh0w)
- [Strategy ideas](/aOes5EpXQ2Kwu13Q7_ZoXA)
- [Single Cell suspension](/qcQt_mBEQyWPi4WEmkrvSQ)
- [Surface Staining](/CT5q82svQaGyby2DB7hNrA)
- [Orders JCBC](https://docs.google.com/spreadsheets/d/1v18K0IBHYYaR5f88SYAchdlgIoIC9hRbk4pDdzkcXtA/edit?usp=sharing)
- [Buffers. pH, 0~2~ & CO~2~](/CLosGNPdQt2F01028ij7iw)
- [DMSO](/pF4zPwDYSH-x5HmwWGKvzw)
- [Contamination](/lErPEwdnQz28zaY1qemnKw)
- [Fixation & ER](/pxsT5W-oRJCE7oxSvLOgJQ)
- [Virus production](/EvCMD-TqRJqA-E7LL9i7hA)
Protocols (*permission required)
---
- [iN Ngn2](https://docs.google.com/document/d/1I7M1cq9pQ_HtCkt3jdSKOCodXDR7f8OD4IB6p2p54pQ/edit?usp=drivesdk)
- [Isolation reference (Beaudoin 2012 & Moutin 2020)](https://docs.google.com/presentation/d/1wSbBv1P5RFNS7nNN7yu13ms5P2YMz2RGbgZ6gUO_Ds0/edit?usp=sharing)
- [Isolation Astrocytes-V9, Rn - Staffan](https://docs.google.com/document/d/1zi1r8-n5VmZIwL95PPTgUsoum3NFljsjArNPer_wxjk/edit?usp=sharing)
- [Isolation Astrocytes-V4, Mm - Staffan](https://docs.google.com/document/d/1Ru3Olqw-HYfqaDzjxQx3IeDRYq_wGRFh80ZFNLXhFXs/edit?usp=sharing)
- [*Isolation OPC, Rn - Adrien.V](https://docs.google.com/document/d/1Dub4-reKxLHXjy5uvMcwAqrDWTPtAP5ihg35x9nQdvc/edit)
- [*Isolation Astrocytes, Mm - Khalil](https://docs.google.com/document/d/194xgbPDHOIA3N8XOn708p2vvytPa94YMnZP4-Xw-ZEo/edit?usp=sharing)
- [*OPC, light stimulation - JohnS](https://docs.google.com/document/d/1iiQI5DcdM3ntzNvm_-cLbnP38wFSxDpgAtAUdOTr_-Y/edit?usp=sharing)
Wet lab
---
- [(qPCR) Primers](https://docs.google.com/spreadsheets/d/1EHpFe397T5TPiemgkPmQfEz3OVhUMAxgdP86VJlzylU/edit?usp=sharing)
- [qPCR-doc](https://docs.google.com/document/d/1kXBZjV7Rn3KzSGHC8SbEgFJkhewtJrXikxfEge_cogQ/edit?usp=sharing)
- [qPCR-sheet](https://docs.google.com/spreadsheets/d/1QTcBdJ7ziEJRK94YxUfdSvod0Fc5tmmfne2GmMCqYkY/edit#gid=532782104)
- [qPCR-Analysis](/35W8ucpRQZKV8D8Guku8Ag)
- [Analysis lipids](https://docs.google.com/presentation/d/1LVsEJLLJlkY0T7-jemTOvxASwcabk7jHxRseIBPlYRc/edit?usp=sharing)
- [outdated- RNA extraction](https://docs.google.com/document/d/1XBmmX-mxCebmCqS3OR2APjttZSLWtogzhShjPXOpxms/edit?usp=sharing)
- [outdated- Reverse transcription](https://docs.google.com/document/d/1jCYtV_Ekb1KWFfNmOtAnhDvmo1ISwzQOWqqhmTQfriw/edit?usp=sharing)
- [outdated- qPCR](https://docs.google.com/document/d/1Qvb_FlHr_UN4eDI4mRAhEx_RhZg4Cj6A3Q_1SBaMDbY/edit?usp=sharing)
- [outdated- IHC for tissue sections](https://docs.google.com/document/d/1n2UwP0zBOicvUnKV6AUgdUWprd_Eq4yT0zy7vJtBpT4/edit?usp=sharing)
- [outdated- Fix and Stain 96 well plate](https://docs.google.com/document/d/1FpyEWuilOxTUfhBF_NRmer8s36kqUYdluOmeX06RMnw/edit?usp=sharing)
Notes
---
- [Ascorbic acid SHT](/396ewE-gSySs4M1RqBYz6g)
- [Isolations SHT](/Oi-_TdQ6R3CqeAIsfflL1Q)
- [<span class="green">Samples Overview</span>](https://docs.google.com/spreadsheets/d/1xOqHmDpMgDkZtPKzw-THfug_3AuYj4xZJABPADCUxB8/edit?usp=sharing)
- [**IsolationX**](https://docs.google.com/presentation/d/1fuXBvO0axfxBuuuBEeQj906offA_u1rcXSpwyYiGlLw/edit?usp=sharing)
- **Isolation-1**
- [<span class="yellow">Slides1</span>](https://docs.google.com/presentation/d/1-Z08Uv8uUUXw1_oY6NAIacGePf5BHlPWvddFAgA9kA0/edit?usp=sharing)
- [Sheet1](https://docs.google.com/spreadsheets/d/1cd3u0zoZ0L9SYEaroGHCSzcilvYTiW4HWqhOl0jpKiw/edit?usp=sharing)
- **Isolation-2**
- [<span class="yellow">Slides2</span>](https://docs.google.com/presentation/d/1caiTf5BUcdfuUkvAmlIRRO42HhUiXKuX78ExdRio7l4/edit?usp=sharing)
- [Sheet2](https://docs.google.com/spreadsheets/d/1uoQnFKTz3SnDJWC1S7Bgse3-RYgRck1Svt6FQN_E18E/edit?usp=sharing)
- **Isolation-3**
- [<span class="yellow">Slides3</span>](https://docs.google.com/presentation/d/1hVa54XJIFLKYfAGcvjA1zwGzJtypIN_3UhhBBlJEb9w/edit?usp=sharing)
- [Sheet3](https://docs.google.com/spreadsheets/d/1oHgXexcbGu2V_ZbBGx2mIPyCJfn5xss3xT8iHTFOLJo/edit?usp=sharing)
- **Isolation-4**
- [<span class="yellow">Slides4</span>](https://docs.google.com/presentation/d/1eWJ6qisa_IbuCujcO6hnabCiUov2HvdSnKHyM_YMsyk/edit?usp=sharing)
- [Sheet4](https://docs.google.com/spreadsheets/d/1aD79NjyYuBAHCzLxjoFLa1E7S87Dt1pdXdcamNktLFw/edit?usp=sharing)
- **Isolation-5**
- [<span class="yellow">Slides5</span>](https://docs.google.com/presentation/d/1hA1DYZVsFZrXiadz_PArYoKjq6c0Es03dMfDD0LeQu8/edit?usp=sharing)
- [Sheet5](https://docs.google.com/spreadsheets/d/1qMWIfKUTMbFmsyKDBK_TsoKdhfvzWXSTrZNAcMTV5lo/edit?usp=sharing)
- **Isolation-6**
- [<span class="yellow">Slides6</span>](https://docs.google.com/presentation/d/15xznoMXxprarqkjfkc0mZQjQwuzo_BVhwahK2Q6FRuw/edit?usp=sharing)
- [Sheet6](https://docs.google.com/spreadsheets/d/1QaVb7J34Nz5_K1QJ_P1yYTAT8rRGeRu1QGt2_rEv2GA/edit?usp=sharing)
- **Isolation-7**
- [<span class="yellow">Slides7</span>](https://docs.google.com/presentation/d/1wzX6zrRhKvs2J8W7OmkHv9eGN4kla7SUkFkl6oaOyHI/edit?usp=sharing)
- [Sheet7](https://docs.google.com/spreadsheets/d/1nQRnDDDOtLKOEg5xCFrxLrVeqk6DV5-KyqD2_pAzegs/edit?usp=sharing)
- **Isolation-8**
- [<span class="yellow">Slides8</span>](https://docs.google.com/presentation/d/1YSNEc3Gp_u9KOyLTOMespLRuVd00GNwjYUYCeIbNCbs/edit?usp=sharing)
- [Sheet8](https://docs.google.com/spreadsheets/d/1e29zlUkIUdJHvE9VwiBuRHkQWz3axxjQVGwOFcLO0mk/edit?usp=sharing)
- **Isolation-9**
- [<span class="yellow">Slides9</span>](https://docs.google.com/presentation/d/1wjLjlTCC9r3ABcpzYhq57PuJDFmVoGfTh3BVXOkTEl8/edit?usp=sharing)
- [Sheet9](https://docs.google.com/spreadsheets/d/1KqIARAFvK2eC9kwHTknoQYwF2H4DD3I2_GpBdSQFdb4/edit?usp=sharing)
- **Isolation-10** Christmas1
- [<span class="yellow">Slides10</span>](https://docs.google.com/presentation/d/1c8A6nKpjov4raa21qC9AeUbD4KMpqOanQIz963HNf5I/edit?usp=sharing)
- **Isolation-11** Christmas-22
- [<span class="yellow">Slides11 - The stained neurons</span>](https://docs.google.com/presentation/d/1bDMR0VyxMI0aprtZo2SM56H8TJizJa2wEprcgbk2u94/edit?usp=sharing)
- **Isolation-12** G1
- [<span class="yellow">Slides12</span>](https://docs.google.com/presentation/d/1O6eBM_lxPMGmWXOksgfzCF2e-xBrBrquJvfdiHN4mCk/edit?usp=sharing)
- **Isolation-13** G2
- [<span class="yellow">Slides13</span>](https://docs.google.com/presentation/d/13P4QW1csJUEcZ84EUrVoTge4rVl70gEajoeE1BgLDk0/edit?usp=sharing)
- **Isolation-14** G3
- [<span class="yellow">Slides14</span>](https://docs.google.com/presentation/d/1LqhjPcQKu0FY1Yvt5lARJYVZH2Tn6sm3xdE5lCy0KGw/edit?usp=sharing)
- **Isolation-15** G4
- [<span class="yellow">Slides15</span>](https://docs.google.com/presentation/d/1Jh8cHhbcOOAgQIb9IcjQCUsMTUeQP-QCdqOzdPvtGKs/edit?usp=sharing)
- **Isolation-16** G5
- [<span class="yellow">Slides16</span>](https://docs.google.com/presentation/d/14KpMJt_S3rl3_55e8uXqk2AZUm0VyEbGPNme9Ueqc0Y/edit?usp=sharing)
- **Isolation-18** ~14 May 2022
- [<span class="yellow">Slides18</span>](https://docs.google.com/presentation/d/14KpMJt_S3rl3_55e8uXqk2AZUm0VyEbGPNme9Ueqc0Y/edit?usp=sharing)
- **Isolation S.1.**
- [<span class="yellow">Slides.S.1.</span>](https://docs.google.com/presentation/d/1VBMrmLdMx3OrQkwZx0Fu3jd4EcdGCHcjy7DlXQKDCMo/edit?usp=sharing)
- **Isolation S.1.**
- [<span class="yellow">Iso-25</span>](https://docs.google.com/presentation/d/1VGmJPtlffzuVcCMqRNh0X6-iDKECntY6XWxDCCobNeA/edit?usp=sharing)
- **GBM lines**
- [<span class="yellow">Slides</span>](https://docs.google.com/presentation/d/1Vm9aAm3d_mzwqA_K3XyHnXRVKINJLR46UMuttT65sTY/edit?usp=sharing)
- [<span class="green">RNAseq</span>](https://docs.google.com/spreadsheets/d/1zaXVY8CToCsRa91p1yXnGI0QwBp8ZYDnr74PvEmMM7M/edit?usp=sharing)
# Experiments
- [OPC generation - Rat Mixed glia](https://docs.google.com/presentation/d/1HJllGzm3sbd9ginyJM47gRc_LgnjGaygRRbvMCMjKD0/edit?usp=sharing)
- [OPC generation - Human EBs](https://docs.google.com/presentation/d/1kymkqDMOxi6ZmYU5ENI6l7EGn1Ce7JgcfyhchOcpw7Q/edit?usp=sharing)
- [Cold-mini brains - EBs @ low temp](https://docs.google.com/presentation/d/1mnfJBaRGaBx9YXrInrvwP-d7DpCwFGSWkHCjxQco_E0/edit?usp=sharing)
- [FA-exp.](https://docs.google.com/presentation/d/1tdj2l9i0-i3Mq0S-BT4gu8Xx1Shyse6DRbU8m-44PPU/edit?usp=sharing)
- [Glucose-exp](https://docs.google.com/presentation/d/1oZ-IdJntdKF1q3p6ABhEZ9-XyLCETWnfgGXBiHsrw3M/edit?usp=sharing)
- [(bbox)siRNA](https://docs.google.com/presentation/d/1FSGEqvKp2j6dKlOtip73aiRhx_vpnpcCa_ZSg4h_OHg/edit?usp=sharing)
- [Temperature](https://docs.google.com/presentation/d/11XNkUdlLqdOUjmonqgYmkikL_TCnMuy9BvFw8WvG_4M/edit?usp=sharing)
# Papers Carnitine treatment
- [Carnitine *in vitro/vivo*](/LFdzRc95QNeW0WcNb3xG2w)
- [Carnitine Nasca-leaks](/DkVF-J_5SGK4hTOuj7P51A)
:::success
# __Change_log__
> __2020-04-11__ First edition.
> [name=MrDr.Staffan]
:::
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