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# System prepended metadata

title: 'Genetic sensors, etc.'
tags: [sensors]

---

---
title: Genetic sensors, etc.
disqus: hackmd
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> Documentation [name=MrDr.Staffan]
###### tags: `sensors` 

### Table of Contents
[TOC]
:::
[Top](#Table-of-Contents)

# Vdac plug
Mitchondrial
[mitoNEET](https://www.eurekalert.org/multimedia/840266)

# SNORCL Oxygen in cells
Produce oxygen in cells by expressing a transporter and a enzyme from bacteria.
https://www.pnas.org/doi/10.1073/pnas.2207955119

# ER modulators
1 µM thapsigargin (TG), which reduces the ER environment by facilitating the influx of reduced glutathione ( Lizak et al., 2020 )

# Micro RNA

## Suclg2 - 
MicroRNA miR-124 expression effect on neuronal cell line
[![](https://hackmd.io/_uploads/SJW0MD7Yh.png)](https://www.ncbi.nlm.nih.gov/geo/tools/profileGraph.cgi?ID=GDS2846:1435841_s_at)
Could be used to silence suclg2?

# Azacitidine
See nucleotides
chemical analog of cytidine, a nucleoside in DNA and RNA.

# 

Can i use reporter for H202 as proxy for mitochondrial activiy.
Mini experiment to show some correlation between the probe and markers for mitochondrial activity. or the numbers of them. or fusion fission proteins?

This could then be used to characterize energetics within single cells in networks with neurons.
or their uptake of glycine vs glutamine.

Cells that take up more glutamine get excited more easily.
Why? beacuse their
other cells take up glycin slc6a11. 


Live mitochondrial number counting. What mt live-dyes. Evaluate the activity of a cell, by other means than Fos. Fos is some kind of genetic activation. THat is fine, but also need to look at mt activity - energetic activation.

Suclg2 



# Klaus-Armin Nave
Ateam1.03^YEMK^
https://www.addgene.org/search/catalog/plasmids/?q=ateam
Genetically encoded fusion protein
Ratiometric [ATP] ~ Fret/CFP

Johannnes Hirrlinger & Andreas Trevisiol

![](https://i.imgur.com/2VhRIOZ.png)

![](https://i.imgur.com/XCJDlGG.png =200x)![](https://i.imgur.com/gZoTg6T.png =250x)

![](https://i.imgur.com/GZEPrFn.png)

![](https://i.imgur.com/UyUzQOo.png)
![](https://i.imgur.com/jj8sPF3.png "inhibited b-oxidation = easier to exhaust ATP by activation")

Mfp2 conditional KO under Plp protomoter (inhibiting Peroxisome-B-oxdiation)

![](https://i.imgur.com/D1Rz5Yp.png)

Looking in cKO Plp1-Slc2a1 mice
![](https://i.imgur.com/i1F14oX.png)

Thinning of the myelin sheet increase
![](https://i.imgur.com/49LvO9P.png)

![](https://i.imgur.com/dparEae.png)
"There is so much more myelin as compared to glycogen"




# NTR nitroreductase - ablation

Inducible Ablation of Astrocytes Shows That These Cells Are Required for Neuronal Survival in the Adult Brain
https://sci-hub.hkvisa.net/10.1002/glia.1061
To study the function of astrocytes in the adult brain, we have targeted
the expression of E. coli nitroreductase (NTR) to the astrocytes of transgenic mice under
the control of the GFAP promoter. The astrocytes expressing NTR were selectively
ablated after administration of the prodrug CB1954, resulting in motor discoordination

[The question is what other consequences are there from the expression that is not only due to the ablation of the astrocytes per se.]



# Aminoadipic acid aAA

[Can be used to ablate specifically astrocytes?](https://www.frontiersin.org/articles/10.3389/fncel.2017.00024/full#:~:text=Figure%202-,FIGURE%202.,-Effects%20and%20dynamics)

[Paper](https://onlinelibrary.wiley.com/doi/epdf/10.1002/%28SICI%291098-1136%28199604%2916%3A4%3C351%3A%3AAID-GLIA7%3E3.0.CO%3B2-2?saml_referrer)
GFAP
Vimentin
Cresyl violet (Nissle stain, basic aniline dye to stain RNA blue, and is used to highlight important structural features of neurons)
Lechtin

Also, the extent of the astrocytic lesions was estimated by measuring the width of the areas devoid of astrocytes (WD)
GFAP and S100b void after aAA

Fig. 3. A Vimentin’ astrocytes are confined to the injection site (the right half of the micrograph) in ASCF animals. B: Vimentin- immunoreactive processes (arrows) and other “debris” fill the area of the injection in OLtlA animals, while Vim+ astrocytes surround the astrocytic lesion.

![](https://i.imgur.com/iBcaf2f.png)
**Ctrl** | aAA ( **B,C** | E, F )
Gfap B, E
S100b C, F
[See Gfap - astrocyte gene](https://hackmd.io/@sholmqvist/BJN5dmhLu/%2F038vIB9uTSGAKcrStOuOHg#Gfap)
[See S100b - astrocyte gene](https://hackmd.io/@sholmqvist/BJN5dmhLu/%2F038vIB9uTSGAKcrStOuOHg#S100b)

![](https://i.imgur.com/DmFdq2t.png "Vimentin Ctrl" =350x) ![](https://i.imgur.com/RUueEtN.png "Vimentin aAA" =350x)
Vimentin in Ctrl | aAA
More in aAA injected. 
[See Vimentin - astrocyte gene](https://hackmd.io/@sholmqvist/BJN5dmhLu/%2F038vIB9uTSGAKcrStOuOHg#Vimentin)

- Vimentin is normally also expressed in endothelial cells.

- Vimentin It is expressed in the mouse in astrocytes and glial precursors well before the onset of GFA protein expression and might therefore serve as an early marker of glial differentiation.

![](https://i.imgur.com/VEyxfM4.png "Ctrl 7 d pi" =350x) ![](https://i.imgur.com/2rdK0Uh.png "aAA 7 d pi" =350x)

Gfap after 7 days. Normal glial scar in ctrl. (left)
Strong glial staining around injection, but intact neurons are still visible within the central astrocyte-free zone

==Does not kill them, but shift them from Gfap&S100b into vimentin expressing astro== what does this signify?==

