---
# System prepended metadata

title: ACE2 Cilia Meeting

---

ACE2 Cilia Meeting
===


:::info
- **Date:** Sept 30, 2022, 1:00pm
- **Agenda**
    1. ESI R01 proposal
    2. Next steps
- **Participants:**
    - Chenxiao
    - Shannon
- **Host:** Shannon
:::

Where were Dr. Eggenschwiler and Riya?


Notes
---


---

:::info
- **Date:** Sept 9, 2022, 1:00pm
- **Agenda**
    1. ESI R01 proposal
    2. Next steps
- **Participants:**
    - Jonathan
    - Chenxiao
    - Shannon
    - Riya
- **Host:** Shannon
:::

ESI R01 Proposal
---

Deadline: Sept 27

[Experiments.docx](https://www.dropbox.com/s/q9s5gbu8pjvylsn/Experiments.docx?dl=0)

[Tentative] Timeline:
 - *Sept 9 - 12*, **all**: Review current draft (`Experiments.docx`), provide any updates to methodologies or approaches.
 - *Sept 13 - 16*, **all**: Convert experiments into Research Strategy.
     - **Drs. Quinn and Eggenschwiler**: budget document and justification
 - *Sept 17 - 21*, **all**: Research Strategy proofreading, finalize figures and tables
     - **Drs. Quinn and Eggenschwiler**: collect PI docs (biosketches, current & pending)
 - *Sept 21 - 27*, **all**: Submit draft to Sponsored Projects, provide feedback, make final submission

Notes
---

Immediate timeframe (by Sept 13)
 - [x] Ask Dr. Lechtreck for letter of support (**Shannon**)
 - [x] Combine `Experiments.docx` into Aim 1 (**Jonathan**)
 - [x] Combine computational goals into Aim 2 (**Shannon**)
 - [x] What kinds of figures do we need? (**Jonathan, Shannon**)
 - [x] Start on budget (**Jonathan, Shannon**)
 - [x] Make a title (**all**)

Title
 - something about ACE2
 - novel high-resolution computational analysis
 - roles of ACE2 in ciliary motility
 - relevance of ACE2 to COVID-19
 - broader relevance of ciliary motion analysis

Figures
 - published data
 - diagrams
 - **what kinds of figures do we need for the experimental / computational aims?**

Modular budget?
 - R01 seems to be limited to $250k/year for *modular* budgets

Letters of support
 - Dr. Lechtreck?

Jonathan can add a lot more experimental detail
 - e.g., in Aim 1, generation of comparator datasets (small molecules that mess with CBF)
     - reality checks
 - e.g., in Aim 2, how knockdowns will be done (lentiviruses)
     - validate extent of knockdown in different shRNAs

**But overall, we're ready to start building the Research Strategy!**


---

:::info
- **Date:** April 29, 2022, 11:00am
- **Agenda**
    1. Update on Chenxiao's work with Dr. Lechtreck
    2. ESI R01 proposal
    3. Chenxiao's fellowship application
    4. FASEB 2022
    5. Next steps
- **Participants:**
    - Jonathan
    - Chenxiao
    - Shannon
    - Riya
- **Host:** Shannon
:::

Update on Chenxiao's work with Dr. Lechtreck
---
Paper that came out recently (March 2022)

![](https://i.imgur.com/8lVSt9V.jpg)

 - Paper seemed a bit rushed but basic idea: different commercial antibodies resulted in different localization, but seemed to recognize several ACE2 isoforms
 - Maybe look into mouse spermatozoa
 - Doug Menke
 - To really take advantage of Karl Lechtreck's expertise: **live imaging**

ESI R01 Proposal
---

Deadline: May 26

Current draft: [dropbox link](https://www.dropbox.com/sh/jlp4wsrc93mxf8f/AABxIK1C9A4MMVQE1N_80qUPa?dl=0)

Both computational AND experimental approaches are changing in each aim
 - Why not use all of them on all of them?

Big Aim 1: all three experimental approaches
 - in Aim 1: the simplest computational approach

In Aim 2: use the Aim 1 dataset with a different (riskier) approach

In Aim 3: use the Aim 1 dataset with a novel (riskiest) approach

Could also just leave the current organization of the Aims, but **make explicit** that we're going to use all methods on all datasets.

Vary lots of experimental parameters
 - different concentrations
 - lots of different pharmacological agents
 - different shRNAs as baselines
 - goal of a continuum of knockdown activity

What are the models going to be? **(Jonathan)**
 - RNAi, shRNA
 - delivery to ex-plants will be trickier than letting cells grow and differentiate

![](https://i.imgur.com/LzltMwF.jpg)

![](https://i.imgur.com/dBWzPtN.jpg)

![](https://i.imgur.com/xD5WvW2.jpg)


Chenxiao's Fellowship Application
---

Deadline: May 15

Editing
 - Jonathan
 - Shannon
 - The Other Dr. Quinn
 - Russ Karls

PI Recommendation Letter

Ask Graduate School for feedback
 - doesn't provide it, of course 🙄

FASEB 2022
---

Current thinking is that we don't have enough data to submit anything. Maybe wait for a later conference?


:dart: Next steps
---

 - ESI R01
     - [ ] Put all the documents on Dropbox **(Shannon)** and send everyone the link
     - [ ] Next week (May 2-6)
         - [ ] **Jonathan, Chenxiao, Riya**: Aim 1A, 1B
         - [ ] **Shannon**: Aim [1|2|3]C
     - [ ] Week after (May 9-13)
         - [ ] **Jonathan, Chenxiao, Riya**: Aim 2A, 2B, 3A, 3B
         - [ ] **Shannon**: Aim [1|2|3]D
     - [ ] May 16-20
         - [ ] **All**: editing
 - Look for fall 2022 / spring 2023 conference venues?

## Notes 
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