ACE2 Cilia Meeting === :::info - **Date:** Sept 30, 2022, 1:00pm - **Agenda** 1. ESI R01 proposal 2. Next steps - **Participants:** - Chenxiao - Shannon - **Host:** Shannon ::: Where were Dr. Eggenschwiler and Riya? Notes --- --- :::info - **Date:** Sept 9, 2022, 1:00pm - **Agenda** 1. ESI R01 proposal 2. Next steps - **Participants:** - Jonathan - Chenxiao - Shannon - Riya - **Host:** Shannon ::: ESI R01 Proposal --- Deadline: Sept 27 [Experiments.docx](https://www.dropbox.com/s/q9s5gbu8pjvylsn/Experiments.docx?dl=0) [Tentative] Timeline: - *Sept 9 - 12*, **all**: Review current draft (`Experiments.docx`), provide any updates to methodologies or approaches. - *Sept 13 - 16*, **all**: Convert experiments into Research Strategy. - **Drs. Quinn and Eggenschwiler**: budget document and justification - *Sept 17 - 21*, **all**: Research Strategy proofreading, finalize figures and tables - **Drs. Quinn and Eggenschwiler**: collect PI docs (biosketches, current & pending) - *Sept 21 - 27*, **all**: Submit draft to Sponsored Projects, provide feedback, make final submission Notes --- Immediate timeframe (by Sept 13) - [x] Ask Dr. Lechtreck for letter of support (**Shannon**) - [x] Combine `Experiments.docx` into Aim 1 (**Jonathan**) - [x] Combine computational goals into Aim 2 (**Shannon**) - [x] What kinds of figures do we need? (**Jonathan, Shannon**) - [x] Start on budget (**Jonathan, Shannon**) - [x] Make a title (**all**) Title - something about ACE2 - novel high-resolution computational analysis - roles of ACE2 in ciliary motility - relevance of ACE2 to COVID-19 - broader relevance of ciliary motion analysis Figures - published data - diagrams - **what kinds of figures do we need for the experimental / computational aims?** Modular budget? - R01 seems to be limited to $250k/year for *modular* budgets Letters of support - Dr. Lechtreck? Jonathan can add a lot more experimental detail - e.g., in Aim 1, generation of comparator datasets (small molecules that mess with CBF) - reality checks - e.g., in Aim 2, how knockdowns will be done (lentiviruses) - validate extent of knockdown in different shRNAs **But overall, we're ready to start building the Research Strategy!** --- :::info - **Date:** April 29, 2022, 11:00am - **Agenda** 1. Update on Chenxiao's work with Dr. Lechtreck 2. ESI R01 proposal 3. Chenxiao's fellowship application 4. FASEB 2022 5. Next steps - **Participants:** - Jonathan - Chenxiao - Shannon - Riya - **Host:** Shannon ::: Update on Chenxiao's work with Dr. Lechtreck --- Paper that came out recently (March 2022) ![](https://i.imgur.com/8lVSt9V.jpg) - Paper seemed a bit rushed but basic idea: different commercial antibodies resulted in different localization, but seemed to recognize several ACE2 isoforms - Maybe look into mouse spermatozoa - Doug Menke - To really take advantage of Karl Lechtreck's expertise: **live imaging** ESI R01 Proposal --- Deadline: May 26 Current draft: [dropbox link](https://www.dropbox.com/sh/jlp4wsrc93mxf8f/AABxIK1C9A4MMVQE1N_80qUPa?dl=0) Both computational AND experimental approaches are changing in each aim - Why not use all of them on all of them? Big Aim 1: all three experimental approaches - in Aim 1: the simplest computational approach In Aim 2: use the Aim 1 dataset with a different (riskier) approach In Aim 3: use the Aim 1 dataset with a novel (riskiest) approach Could also just leave the current organization of the Aims, but **make explicit** that we're going to use all methods on all datasets. Vary lots of experimental parameters - different concentrations - lots of different pharmacological agents - different shRNAs as baselines - goal of a continuum of knockdown activity What are the models going to be? **(Jonathan)** - RNAi, shRNA - delivery to ex-plants will be trickier than letting cells grow and differentiate ![](https://i.imgur.com/LzltMwF.jpg) ![](https://i.imgur.com/dBWzPtN.jpg) ![](https://i.imgur.com/xD5WvW2.jpg) Chenxiao's Fellowship Application --- Deadline: May 15 Editing - Jonathan - Shannon - The Other Dr. Quinn - Russ Karls PI Recommendation Letter Ask Graduate School for feedback - doesn't provide it, of course 🙄 FASEB 2022 --- Current thinking is that we don't have enough data to submit anything. Maybe wait for a later conference? :dart: Next steps --- - ESI R01 - [ ] Put all the documents on Dropbox **(Shannon)** and send everyone the link - [ ] Next week (May 2-6) - [ ] **Jonathan, Chenxiao, Riya**: Aim 1A, 1B - [ ] **Shannon**: Aim [1|2|3]C - [ ] Week after (May 9-13) - [ ] **Jonathan, Chenxiao, Riya**: Aim 2A, 2B, 3A, 3B - [ ] **Shannon**: Aim [1|2|3]D - [ ] May 16-20 - [ ] **All**: editing - Look for fall 2022 / spring 2023 conference venues? ## Notes <!-- Other important details discussed during the meeting can be entered here. -->