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tags: ebook
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# Helpful Videos and links

## How Nanopore sequencing works

<iframe width="560" height="315" src="https://www.youtube.com/embed/RcP85JHLmnI" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe>
MinION Kk1B

### Nanopore Supporting Material-requires a free Nanopore account
[Creating an account to access Nanopore content](https://help.nanoporetech.com/en/articles/6505425-how-do-i-create-an-account)
In order to access valuable Nanopore resources, one must create a free account to view their documentation.

[Nanopore sequencing kits](https://store.nanoporetech.com/us/sequencing-kits.html)
This link shows all the available Nanopore kits

[Nanopore protocols](https://community.nanoporetech.com/docs/prepare/library_prep_protocols)
This is the direct link to protocols for the different Nanopore kits

#### IT Requirements
-[MinION Mk1B](https://community.nanoporetech.com/requirements_documents/minion-it-reqs.pdf)

[Nanopore sequencing device selection](https://nanoporetech.com/resource-centre/video/ncm22/which-nanopore-sequencing-device-to-choose) Video introduces the different available Nanopore devices and selection of correct flow cell.

#### DNA extraction and quantification

[DNA extraction methods](https://dnabarcoding101.org/pWoKf2UpS0aNxXfKFTAZ0Q)
Provides different methods for DNA extraction such as Rapid, silica, Chelex, and Qiagen DNA isolation.

[Online weight to molar quantity calculator for nucleic acids](https://www.bioline.com/media/calculator/01_07.html) This is a useful calculator to convert from ug/ul of DNA to fM.

Library prep selection tool
<iframe width="560" height="315" src="https://www.youtube.com/embed/LFsyfU4e-Iw" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe>

##### Laboratory equipment, consumables, and requirements

- [RNA](https://community.nanoporetech.com/requirements_documents/rna-consumables.pdf)
- Ligation
[https://community.nanoporetech.com/requirements_documents/ligation-consumables.pdf]

- Rapid
[https://community.nanoporetech.com/requirements_documents/rapid-consumables.pdf]

How to perform quality control on Nanopore flow cell and what to do if the number of active pores is below expected

<iframe width="560" height="315" src="https://www.youtube.com/embed/OyfyU9MfBpw" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe>

[Analysis of PCR products with gel electrophoresis](https://dnabarcoding101.org/lab/protocol-4.html)

Nanopore Technologies Rapid Barcode kit Library preparation protocol. Note: kit to be discontinued but video provides a nice overview of steps <iframe width="560" height="315" src="https://www.youtube.com/embed/P5y4lfpCUtw" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe>