# Adoncholaimus thalassophygas mitochondrion - Table of Content [ToC] # 1-) MBG run With parameters MBG -k 1001 -w 250 -a 5 -u 150 With all reads: ``` /lustre/scratch123/tol/teams/blaxter/users/ek5/SW9/E5/E5_hifireads.fasta.gz ``` ![](https://i.imgur.com/jpWw7It.png) ``` >72<11>13>15>68<71>69<12>13>15>68<71>69<12>13>15>68<71>69<12>13>15>68<71>69<12>13>15>68<71>69<12>13>15>68<71>69<12<16 177 ``` ## 1.1-) MBG run With parameters MBG -k 1401 -w 250 -a 5 -u 150 ![](https://i.imgur.com/613Yp1c.png) # 2 - When I look at the mapping of reads back to the assembled mito from assembly, it doesnt seem to me to be correct. ![](https://i.imgur.com/mY8pRDj.png) ![](https://i.imgur.com/obyMHyH.png) But maybe the mito is 16kb as seem from the third mapping plot above. # 3 . Mapping ![](https://i.imgur.com/oYYju6Z.png) ![](https://i.imgur.com/oHjM8qZ.png) Took all the reads mapped above and ran a MBG ![](https://i.imgur.com/lHyJbje.png) Then the image bellow is a nucmer plot of erna's 26Kb sequence, with the sequence 1 from the MBG graph above. ![](https://i.imgur.com/8051GUQ.png) Annotating the 1.fa contig with Mitos. ![](https://i.imgur.com/r0uojJx.png)