---
# System prepended metadata

title: Sloth tRNAs
tags: [sloth]

---

# Sloth tRNAs

###### tags: `sloth`


- Table of Content
[ToC]


# 1. tRNA Mutations
[Google Sheets Full List] (https://docs.google.com/spreadsheets/d/1OF9EL9bv6sxIRzERxV1s8B3YLUkGNKzwL80pvT81aFU/edit#gid=0) of Mutations Checked

Jacqui Johnson's table

| Mutation                                                                    | Conserved    | tRNA | Disease                                                                                                                                                                                                                                                                                                                                                                                                                                          | B. pygmaeus | B. torquatus | B. tridactylus | B. variegatus | C. didactylus | C. hoffmanni | Notes                            | MitoTip                                                                                |
| --------------------------------------------------------------------------- | --- | ---- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ | ----------- | ------------ | -------------- | ------------- | ------------- | ------------ | -------------------------------- | -------------------------------------------------------------------------------------- |
| m.4301A>C                                                                   |  97%   | ile  | [MELAS (A>T)](https://www.nature.com/articles/ejhg2011262)                                                                                                                                                                                                                                                                                                                                                                                       | C           | C            | C              | -             | -             | -            |                                  | [Likely Pathogenic](https://www.mitomap.org/cgi-bin/mitotip?pos=4301&alt=C&quart=2)    |
| m.4311G>A                                                                   | 93%    | ile  |                                                                                                                                                                                                                                                                                                                                                                                                                                                  | -           | -            | -              | -             | A             | A            |                                  | [Likely Pathogenic](https://www.mitomap.org/cgi-bin/mitotip?pos=4311&alt=A&quart=1)    |
| m.5788T>G                                                                   | 93%    | cys  |                                                                                                                                                                                                                                                                                                                                                                                                                                                  | G           | G            | G              | G             | G             | G            | In most sloths, no other species | [Likely Benign](https://www.mitomap.org/cgi-bin/mitotip?pos=5788&alt=G&quart=4)        |
| m.5789T>C                                                                   | 82%    | cys  | [NARP (T>C)](https://www.mitomap.org/cgi-bin/print_ref_list?refs=92530&title=Variant%20T5789C%20at%205789)                                                                                                                                                                                                                                                                                                                                       | C           | C            | C              | C             | C             | C            | All Pilosa, some other mammals   | [Likely Pathogenic](https://www.mitomap.org/cgi-bin/mitotip?pos=5789&alt=C&quart=1)    |
| m.7551T>C                                                                   |93%     | asp  | [Deafness (T>C)](https://mitomap.org/cgi-bin/print_ref_list?refs=90296,90739,2581,90105,90507,91074,3532,90508,90863,90209,90261,90510,91076,91071,91525,91327,92376,92367,92370,92056,91923,92162,10498,92373&title=Variant%20T7511C%20at%207511)   - onset ~14yo                                                                                                                                                                               | C           | C            | C              | -             | -             | -            |                                  | [Possibly Benign](https://www.mitomap.org/cgi-bin/mitotip?pos=7551&alt=C&quart=3)      |
| m.14709T>C |  95%   | glu  | [MM+DMDF / Encephalomyopathy / Deafness / Dementia+diabetes+ophthalmoplegia (T>C)](https://www.mitomap.org/cgi-bin/print_ref_list?refs=688,689,2001,3452,90271,90571,3700,90267,3427,3451,90261,4244,4439,5458,90637,92188,91257,91327,91639,91640,92376,8855,92362,9747,10498,92324,92363,92333&title=Variant%20T14709C%20at%2014709) - [Phosphorylation Deficiency Visualization (p20)](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4606788/) | C           | -            | -              | C             | -             | -            |                                  | [Possibly Pathogenic](https://www.mitomap.org/cgi-bin/mitotip?pos=14709&alt=C&quart=2) |
| m.14728T>C                                                                  |   91%  | glu  | [Late-onset mitochondrial encephalomyopathy (T>C)](https://www.sciencedirect.com/science/article/abs/pii/S1567724907001201?via%3Dihub)                                                                                                                                                                                                                                                                                                           | -           | -            | -              | -             | C             | C            |                                  | [Possibly Benign](https://www.mitomap.org/cgi-bin/mitotip?pos=14728&alt=C&quart=3)     |


## 1.1. 2D structures

- transcribed dna to rna https://github.com/marcelauliano/phython_scripts/blob/master/dna2rna.py

- predicted 2D tRNA structure with https://rnacentral.org/r2dt?jobid=r2dt-R20230605-140857-0138-66623650-p1m https://www.nature.com/articles/s41467-021-23555-5 

### 1.1.1 tRNA ile

Below are the predicted 2D structures. They are basically the same for the species that have the mutation and the ones that don't. Sequence variaton exists.

```

>NC_028554.1_Bradypus pygmaeus-ile
(((((((..((((....)))).(((((.......))))).....(((((........)))))))))))).
>KT818523.1-Bradypus_pygmaeus_ile
(((((((..((((....)))).(((((.......))))).....(((((........)))))))))))).
>NC_028555.1_Bradypus_torquatus_ile
(((((((..((((....)))).(((((.......))))).....(((((........)))))))))))).
>NC_037527.1_Bradypus_tridactylus_ISEM_T5013_ile
(((((((..((((....)))).(((((.......))))).....(((((........)))))))))))).
>NC_028501.1_Bradypus_variegatus_MVZ_155186_ile
(((((((..((((....)))).(((((.......))))).....(((((........)))))))))))).
>KT818537.1_Choloepus_didactylus_MNHN_19981819_ile
(((((((..((((....)))).(((((.......))))).....(((((.......)))))))))))).
>KR336793.1_Choloepus_hoffmanni_ile
(((((((..((((....)))).(((((.......))))).....(((((.......)))))))))))).
```


| Bradypus torquatus ile | Bradypus pygmaeus ile |  |
| -------- | -------- | -------- |
| ![](https://hackmd.io/_uploads/r1-77PjI3.png)     | ![](https://hackmd.io/_uploads/B1U9fPo82.png)    | 
| Choloepus didactylus ile |
![](https://hackmd.io/_uploads/H1ZZ7DjU3.png) | 


### 1.1.2 tRNA cys

```
>Bradypus variegatus
(((((((..((((.....)))).(((((.......)))))....(((((.......)))))))))))).
>Carnivora_Mustela_erminea
((((((..((((....)))).(((((.......)))))....(((((......)))))))))))..
```



| Bradypus variegatus cys | Mustela erminea|
| ----- | ----- |
| ![](https://hackmd.io/_uploads/BycEswj83.png) | ![](https://hackmd.io/_uploads/SyNMaws82.png)
 |

