# Sloth tRNAs

###### tags: `sloth`

- Table of Content
[ToC]

# 1. tRNA Mutations
[Google Sheets Full List] (https://docs.google.com/spreadsheets/d/1OF9EL9bv6sxIRzERxV1s8B3YLUkGNKzwL80pvT81aFU/edit#gid=0) of Mutations Checked

Jacqui Johnson's table

| Mutation | Conserved | tRNA | Disease | B. pygmaeus | B. torquatus | B. tridactylus | B. variegatus | C. didactylus | C. hoffmanni | Notes | MitoTip |
| --------------------------------------------------------------------------- | --- | ---- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ | ----------- | ------------ | -------------- | ------------- | ------------- | ------------ | -------------------------------- | -------------------------------------------------------------------------------------- |
| m.4301A>C | 97% | ile | [MELAS (A>T)](https://www.nature.com/articles/ejhg2011262) | C | C | C | - | - | - | | [Likely Pathogenic](https://www.mitomap.org/cgi-bin/mitotip?pos=4301&alt=C&quart=2) |
| m.4311G>A | 93% | ile | | - | - | - | - | A | A | | [Likely Pathogenic](https://www.mitomap.org/cgi-bin/mitotip?pos=4311&alt=A&quart=1) |
| m.5788T>G | 93% | cys | | G | G | G | G | G | G | In most sloths, no other species | [Likely Benign](https://www.mitomap.org/cgi-bin/mitotip?pos=5788&alt=G&quart=4) |
| m.5789T>C | 82% | cys | [NARP (T>C)](https://www.mitomap.org/cgi-bin/print_ref_list?refs=92530&title=Variant%20T5789C%20at%205789) | C | C | C | C | C | C | All Pilosa, some other mammals | [Likely Pathogenic](https://www.mitomap.org/cgi-bin/mitotip?pos=5789&alt=C&quart=1) |
| m.7551T>C |93% | asp | [Deafness (T>C)](https://mitomap.org/cgi-bin/print_ref_list?refs=90296,90739,2581,90105,90507,91074,3532,90508,90863,90209,90261,90510,91076,91071,91525,91327,92376,92367,92370,92056,91923,92162,10498,92373&title=Variant%20T7511C%20at%207511) - onset ~14yo | C | C | C | - | - | - | | [Possibly Benign](https://www.mitomap.org/cgi-bin/mitotip?pos=7551&alt=C&quart=3) |
| m.14709T>C | 95% | glu | [MM+DMDF / Encephalomyopathy / Deafness / Dementia+diabetes+ophthalmoplegia (T>C)](https://www.mitomap.org/cgi-bin/print_ref_list?refs=688,689,2001,3452,90271,90571,3700,90267,3427,3451,90261,4244,4439,5458,90637,92188,91257,91327,91639,91640,92376,8855,92362,9747,10498,92324,92363,92333&title=Variant%20T14709C%20at%2014709) - [Phosphorylation Deficiency Visualization (p20)](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4606788/) | C | - | - | C | - | - | | [Possibly Pathogenic](https://www.mitomap.org/cgi-bin/mitotip?pos=14709&alt=C&quart=2) |
| m.14728T>C | 91% | glu | [Late-onset mitochondrial encephalomyopathy (T>C)](https://www.sciencedirect.com/science/article/abs/pii/S1567724907001201?via%3Dihub) | - | - | - | - | C | C | | [Possibly Benign](https://www.mitomap.org/cgi-bin/mitotip?pos=14728&alt=C&quart=3) |

## 1.1. 2D structures

- transcribed dna to rna https://github.com/marcelauliano/phython_scripts/blob/master/dna2rna.py

- predicted 2D tRNA structure with https://rnacentral.org/r2dt?jobid=r2dt-R20230605-140857-0138-66623650-p1m https://www.nature.com/articles/s41467-021-23555-5

### 1.1.1 tRNA ile

Below are the predicted 2D structures. They are basically the same for the species that have the mutation and the ones that don't. Sequence variaton exists.

>NC_028554.1_Bradypus pygmaeus-ile
(((((((..((((....)))).(((((.......))))).....(((((........)))))))))))).
>KT818523.1-Bradypus_pygmaeus_ile
(((((((..((((....)))).(((((.......))))).....(((((........)))))))))))).
>NC_028555.1_Bradypus_torquatus_ile
(((((((..((((....)))).(((((.......))))).....(((((........)))))))))))).
>NC_037527.1_Bradypus_tridactylus_ISEM_T5013_ile
(((((((..((((....)))).(((((.......))))).....(((((........)))))))))))).
>NC_028501.1_Bradypus_variegatus_MVZ_155186_ile
(((((((..((((....)))).(((((.......))))).....(((((........)))))))))))).
>KT818537.1_Choloepus_didactylus_MNHN_19981819_ile
(((((((..((((....)))).(((((.......))))).....(((((.......)))))))))))).
>KR336793.1_Choloepus_hoffmanni_ile
(((((((..((((....)))).(((((.......))))).....(((((.......)))))))))))).

| Bradypus torquatus ile | Bradypus pygmaeus ile | |
| -------- | -------- | -------- |
|  |  |
| Choloepus didactylus ile |
 |

### 1.1.2 tRNA cys

>Bradypus variegatus
(((((((..((((.....)))).(((((.......)))))....(((((.......)))))))))))).
>Carnivora_Mustela_erminea
((((((..((((....)))).(((((.......)))))....(((((......)))))))))))..

| Bradypus variegatus cys | Mustela erminea|
| ----- | ----- |
|  | 
|