# ELAEVO Students!! Help me find my mistake I just got back from the field, I made a terrible mistake. I had a very valuable RNA sample that I had accidentally left out in the sun for 3 days. I'm sure it is ruined.. :( My lab technician made an even worse mistake. While preparing the sample for sequencing, he might have mis-labeled the samples and now I don't know which is the good sample and which is the bad sample. Can you help me figure out which is the good sample and which is the bad sample, by any means possible? You may choose to assemble these transcriptomes, or do something else. There are about 12 million reads in each dataset. The 1st set of reads is on Ron at ``` /home/unhMM/matt/example_reads/mystery_sample_A.R1.fq /home/unhMM/matt/example_reads/mystery_sample_A.R2.fq ``` The second set of reads are at: ``` /home/unhMM/matt/example_reads/mystery_sample_B.R1.fq /home/unhMM/matt/example_reads/mystery_sample_B.R2.fq ``` ### One critical difference from yesterday!!! Before you start your work, enter this command. (I can explain, but do it) ``` tmux new -s elaevo ``` If you log out of the server or get disconnected and want to log back in to check ot see if your assembly is done (it will take several hours) ``` tmux attach -t elaevo ``` ### A few more instructions. For this assembly, you can set `CPU=16` and `MEM=100` This is optional, but there is a prize for the 1st team to tell me which is the bad sample, and why.. You may work in groups. We will announce the winner tomorrow at the asado.