---
# System prepended metadata

title: Fucking Bacteria Tests
tags: [共筆]

---

[魯國共筆目錄](/f422AdmtSGeEnkS-LwYuqA)
# Fucking Bacteria Tests
###### tags: `共筆`
:::info
請善用`ctrl+F`搜尋
:::
:::danger
請以老師講義為準，此表僅供參考
:::
## Catalase
|Catalase(+)|Catalase(-)|
|----|----|
|Staphylococcus<br>Listeria monocytogenes<br>Corynebacterium<br>Nocardia<br>Neisseriaceae<br>Enterobacteriaceae<br>Aeromonas <br>Brucella<br>Propionibacterium<br>Bacteroides fragilis|Streptococcus<br>Enterococcus<br>Erysipelothrix rhusiopathiae<br>Treponema|
## Oxidase
|Oxidase(+)|Oxidase(-)|
|----|----|
|Neisseriaceae<br>Vibrio<br>Aeromonas<br>Pseudomonas aeruginosa |Enterobacteriaceae<br>>Salmonella enterica<br>>Shigella<br>>Yersinia<br>|
## Streptococcus Classify
|PYR(+)|CAMP(+)|
|----|----|
|S. pyogenes(Group A)<br>E. faecalis(Group D)|S. agalactiae(Group B)|
* Listeria monocytogenes: CAMP(+)

||Bacitracin|Optochin|
|---|---|---|
|S. pyogenes|Sensitive|Resistant|
|S. agalactiae|Resistant|Resistant|
|S. pneumoniae|Resistant|Sensitive|
## Hippurate
|Hippurate(+)|
|---|
|Streptococcus agalactiae<br>Listeria monocytogenes<br>Campylobacter jejuni|
## Hemolytic
|α-hemolytic|β-hemolytic|nonhemolytic<br>(γ-hemolytic)|
|---|---|---|---|
|Viridans Streptococcus<br>>Streptococcus pneumoniae<br>>Streptococcus mitis<br>Erysipelothrix rhusiopathiae|Streptococcus pyogenes<br>Streptococcus agalactiae<br>Listeria monocytogenes **(weakly)**<br>Pseudomonas aeruginosa |Bacillus anthracis|

## Capsule
|Capsule(+)|Capsule(-)|
|---|---|
|Streptococcus pyogenes<br>Streptococcus agalactiae<br>Streptococcus pneumoniae<br>Bacillus anthracis<font color="red">(Polypeptide)</font><br><font color="orange">Neisseria meningitidis</font><br>Enterobacteriaceae<br><font color="blue">Vibrio vulnificus</font><br>Pseudomonas aeruginosa(<font color="green">Mucoid</font>)<br>Haemophilus influenzae<br>>vaccine:<font color="red">PRP</font>(polyribitol phosphate) in capsule)<br>Francisella tularensis<br>Bacteroides fragilis|Erysipelothrix rhusiopathiae<br><font color="orange">Neisseria gonorrhoeae</font><br><font color="blue">Vibrio cholerae<br>Vibrio parahaemolyticus</font>|
## Salt, Bile
|Salt|Bile|
|---|---|
|Staphylococcus aureus<br>Enterococcus<br>Listeria monocytogenes<br>Vibrio(Require salt for growth)<br>>V. cholerae:可生長於無鹽環境<br>Aeromonas hydrophila<br>|Enterococcus<br>Listeria monocytogenes<br>Enterobacteriaceae<br>Vibrio<br>Bacteroides fragilis|
S. pneumoniae are <font color="blue">bile soluble</font>.
## Agar
* Erysipelothrix rhusiopathiae: produces <font color="red">H~2~S</font> on triple sugar iron (**TSI**) agar slant 
* Corynebacterium diphtheriae: Loeffler‘s serum agar slant
                               Cysteine-**tellurite** agar
* Nocardia: **BCYE**(buffered charcol yeast extract) agar 
* Mycobacterium: Löwenstein-Jensen agar 
* Neisseria gonorrhoeae: Thayer-Martin(**TM**) medium
* Shigella: Hektoen enteric agar -> green colonies
* Salmonella: Hektoen enteric agar -> black colonies
* Vibrio: Thiosulfate Citrate Bile salt Sucrose agar (**TCBS**)
* Vibrio parahaemolyticus: **human blood**(not sheep blood); ==<font color="red">Kanagawa(+)</font>==
* Campylobacter: Campy-BAP
* Haemophilus: Chocolate agar, Staphylococcus aureus lysis RBC(<font color="red">X, V factor</font>)
* Bordetella Pertussis: no cotton + Regan-Lowe agar 
* F. tularensis: **Cysteine**-supplemented media (e.g., chocolate agar, BCYE agar)
* Legionella: <font color="red">Buffered charcoal yeast extract (BCYE) agar</font> (require **L-cysteine** and **iron salts**)
* Bacteroides fragilis: Bacteroides bile-esculin (BBE) agar
    * is stimulated by <font color="red">bile</font>, resistant to **gentamicin**, and able to hydrolyze <font color="red">esculin</font>, producing a **black precipitate**)
## Temperature
* Enterococcus: 10-45℃
* Yersinia pestis: 4℃
* Listeria monocytogenes: 4℃
* Campylobacter jejuni: 42℃
* Pseudomonas aeruginosa: 4~42℃
## Motility
|Motile|Nonmotile|
|---|---|
|<font color ="green">Bacillus cereus</font><br>Listeria monocytogenes<br><font color="red">Enterobacteriaceae(mostly)<br>>Escherichia coli<br>>Salmonella enterica</font><br>Vibrio<br>Helicobacter pylori(highly)<br>Pseudomonas aeruginosa<br>Clostridium tetani<br>Treponema|<font color ="green">Bacillus anthracis</font><br>Erysipelothrix rhusiopathiae<br>Corynebacterium diphtheriae<br>Mycobacterium<br>Neisseriaceae gonorrhoeae<br><font color="red">Enterobacteriaceae(except)<br>>Shigella<br>>Klebsiella pneumoniae<br>>Yersinia</font><br>Bordetella Pertussis<br>Brucella<br>Clostridium perfringens<br>Propionibacterium|
## Intracellular parasite
|Facultative|Obligate|
|---|---|
|Listeria monocytogenes<br>Mycobacterium<br>Salmonella enterica<br>Yersinia<br>Brucella<br>Francisella tularensis<br> Legionella pneumophila|Chlamydia<br>Rickettsia|