[魯國共筆目錄](/f422AdmtSGeEnkS-LwYuqA) # Fucking Bacteria Tests ###### tags: `共筆` :::info 請善用`ctrl+F`搜尋 ::: :::danger 請以老師講義為準,此表僅供參考 ::: ## Catalase |Catalase(+)|Catalase(-)| |----|----| |Staphylococcus<br>Listeria monocytogenes<br>Corynebacterium<br>Nocardia<br>Neisseriaceae<br>Enterobacteriaceae<br>Aeromonas <br>Brucella<br>Propionibacterium<br>Bacteroides fragilis|Streptococcus<br>Enterococcus<br>Erysipelothrix rhusiopathiae<br>Treponema| ## Oxidase |Oxidase(+)|Oxidase(-)| |----|----| |Neisseriaceae<br>Vibrio<br>Aeromonas<br>Pseudomonas aeruginosa |Enterobacteriaceae<br>>Salmonella enterica<br>>Shigella<br>>Yersinia<br>| ## Streptococcus Classify |PYR(+)|CAMP(+)| |----|----| |S. pyogenes(Group A)<br>E. faecalis(Group D)|S. agalactiae(Group B)| * Listeria monocytogenes: CAMP(+) ||Bacitracin|Optochin| |---|---|---| |S. pyogenes|Sensitive|Resistant| |S. agalactiae|Resistant|Resistant| |S. pneumoniae|Resistant|Sensitive| ## Hippurate |Hippurate(+)| |---| |Streptococcus agalactiae<br>Listeria monocytogenes<br>Campylobacter jejuni| ## Hemolytic |α-hemolytic|β-hemolytic|nonhemolytic<br>(γ-hemolytic)| |---|---|---|---| |Viridans Streptococcus<br>>Streptococcus pneumoniae<br>>Streptococcus mitis<br>Erysipelothrix rhusiopathiae|Streptococcus pyogenes<br>Streptococcus agalactiae<br>Listeria monocytogenes **(weakly)**<br>Pseudomonas aeruginosa |Bacillus anthracis| ## Capsule |Capsule(+)|Capsule(-)| |---|---| |Streptococcus pyogenes<br>Streptococcus agalactiae<br>Streptococcus pneumoniae<br>Bacillus anthracis<font color="red">(Polypeptide)</font><br><font color="orange">Neisseria meningitidis</font><br>Enterobacteriaceae<br><font color="blue">Vibrio vulnificus</font><br>Pseudomonas aeruginosa(<font color="green">Mucoid</font>)<br>Haemophilus influenzae<br>>vaccine:<font color="red">PRP</font>(polyribitol phosphate) in capsule)<br>Francisella tularensis<br>Bacteroides fragilis|Erysipelothrix rhusiopathiae<br><font color="orange">Neisseria gonorrhoeae</font><br><font color="blue">Vibrio cholerae<br>Vibrio parahaemolyticus</font>| ## Salt, Bile |Salt|Bile| |---|---| |Staphylococcus aureus<br>Enterococcus<br>Listeria monocytogenes<br>Vibrio(Require salt for growth)<br>>V. cholerae:可生長於無鹽環境<br>Aeromonas hydrophila<br>|Enterococcus<br>Listeria monocytogenes<br>Enterobacteriaceae<br>Vibrio<br>Bacteroides fragilis| S. pneumoniae are <font color="blue">bile soluble</font>. ## Agar * Erysipelothrix rhusiopathiae: produces <font color="red">H~2~S</font> on triple sugar iron (**TSI**) agar slant * Corynebacterium diphtheriae: Loeffler‘s serum agar slant Cysteine-**tellurite** agar * Nocardia: **BCYE**(buffered charcol yeast extract) agar * Mycobacterium: Löwenstein-Jensen agar * Neisseria gonorrhoeae: Thayer-Martin(**TM**) medium * Shigella: Hektoen enteric agar -> green colonies * Salmonella: Hektoen enteric agar -> black colonies * Vibrio: Thiosulfate Citrate Bile salt Sucrose agar (**TCBS**) * Vibrio parahaemolyticus: **human blood**(not sheep blood); ==<font color="red">Kanagawa(+)</font>== * Campylobacter: Campy-BAP * Haemophilus: Chocolate agar, Staphylococcus aureus lysis RBC(<font color="red">X, V factor</font>) * Bordetella Pertussis: no cotton + Regan-Lowe agar * F. tularensis: **Cysteine**-supplemented media (e.g., chocolate agar, BCYE agar) * Legionella: <font color="red">Buffered charcoal yeast extract (BCYE) agar</font> (require **L-cysteine** and **iron salts**) * Bacteroides fragilis: Bacteroides bile-esculin (BBE) agar * is stimulated by <font color="red">bile</font>, resistant to **gentamicin**, and able to hydrolyze <font color="red">esculin</font>, producing a **black precipitate**) ## Temperature * Enterococcus: 10-45℃ * Yersinia pestis: 4℃ * Listeria monocytogenes: 4℃ * Campylobacter jejuni: 42℃ * Pseudomonas aeruginosa: 4~42℃ ## Motility |Motile|Nonmotile| |---|---| |<font color ="green">Bacillus cereus</font><br>Listeria monocytogenes<br><font color="red">Enterobacteriaceae(mostly)<br>>Escherichia coli<br>>Salmonella enterica</font><br>Vibrio<br>Helicobacter pylori(highly)<br>Pseudomonas aeruginosa<br>Clostridium tetani<br>Treponema|<font color ="green">Bacillus anthracis</font><br>Erysipelothrix rhusiopathiae<br>Corynebacterium diphtheriae<br>Mycobacterium<br>Neisseriaceae gonorrhoeae<br><font color="red">Enterobacteriaceae(except)<br>>Shigella<br>>Klebsiella pneumoniae<br>>Yersinia</font><br>Bordetella Pertussis<br>Brucella<br>Clostridium perfringens<br>Propionibacterium| ## Intracellular parasite |Facultative|Obligate| |---|---| |Listeria monocytogenes<br>Mycobacterium<br>Salmonella enterica<br>Yersinia<br>Brucella<br>Francisella tularensis<br> Legionella pneumophila|Chlamydia<br>Rickettsia|
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