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# System prepended metadata

title: 'Plant Microbe Interaction :bug:'

---

# Plant Microbe Interaction :bug: 
###### tags: 2022/02/28~2022/03/04, group project

## Tomato late blight (Phytophthora infestans)

![](https://i.imgur.com/qU2FOny.jpg)
![](https://i.imgur.com/YYIugN1.jpg)
![](https://i.imgur.com/wAl00wm.jpg)

## <font color=Blue> Background information </font>

**1. Cultivars**

| Cultivar | Gene | Other information |
| -------- | -------- | -------- |
| *Solanum lycopersicum*    |     |   commercial variety  |
| *Solanum pimpinellifolium* | ph1, ph2, ph3, ph4, ph5 | wild type |

**2. Strains pathogens to focus on**

| Name | Other information | 
| -------- | -------- | 
| US20     |     |
| US21    |     |
| US22    |     |
| US23    |     |


**3. 3 PGPR**

| ID | Name | Efficiency (green house) | 
| -------- | -------- | -----|
| PGPR1     | *Paenibacillus polymyxa AB15*   |74% |
| PGPR2    | *Bacillus pumilus SE34*     | 42%|
| PGPR3    | *Pseudomonas fluorescens SS101*    |80% |

**4. 2 Elicitor**

| ID | Name | Efficiency |
| -------- | -------- | -------- |
| E1     | Chitosan     |Reduce in vitro mycelium growth at 78% | 
| E2    | Algal  | Reduce 36% disease severity of *Sargassum fusiforme* |

------------

## <font color=blue> Protocol </font>

**1. 5 Cultivars**

| Name | Gene | Cultivar | Company| Other infor mation |
| -------- | -------- | -------- |-------- |-------- |
| C1    | ph2/ph3     | Pruden's Purple    |Johnny’s selected seed |
| C2    | ph3    | Plum Regal   | Johnny’s selected seed | ph3 is the major gene|
| C3    | ph2     |  *Solanum lycopersicum moboline*  | Erptomato | T1|
| C4    | ph1, ph2, ph3, ph4, ph5     | *Solanum pimpinellifolium*     | scientists | might have the best result|
| C5 (control)    | Nothing     | *Solanum lycopersicum var. Rumba* | farmers |  |

**2. 35 PGPRs**

| ID | Treatment | 
| -------- | -------- | 
| 1     | C1* PGPR1    |
| 2    | C2* PGPR1   |
| 3    | C3* PGPR1    |
| 4   | C4* PGPR1   |
| 5    | C5* PGPR1    |
| 6     | C1* PGPR2    |
| 7    | C2* PGPR2   |
| 8    | C3* PGPR2    |
| 9   | C4* PGPR2   |
| 10    | C5* PGPR2    |
| 11     | C1* PGPR3    |
| 12    | C2* PGPR3   |
| 13    | C3* PGPR3    |
| 14   | C4* PGPR3   |
| 15    | C5* PGPR3    |
| 16    | C1* PGPR1/ PGPR2    |
| 17     | C2* PGPR1/ PGPR2    |
| 18    | C3* PGPR1/ PGPR2   |
| 19    | C4* PGPR1/ PGPR2    |
| 20   | C5* PGPR1/ PGPR2   |
| 21    | C1* PGPR1/ PGPR3    |
| 22     | C2* PGPR1/ PGPR3    |
| 23    | C3* PGPR1/ PGPR3   |
| 24    | C4* PGPR1/ PGPR3    |
| 25   | C5* PGPR1/ PGPR3   |
| 26    | C1* PGPR2/ PGPR3    |
| 27     | C2* PGPR2/ PGPR3    |
| 28    | C3* PGPR2/ PGPR3   |
| 29    | C4* PGPR2/ PGPR3    |
| 30   | C5* PGPR2/ PGPR3   |
| 31    | C1* PGPR1/PGPR2/ PGPR3    |
| 32     | C2* PGPR1/PGPR2/ PGPR3    |
| 33    | C3* PGPR1/PGPR2/ PGPR3   |
| 34    | C4* PGPR1/PGPR2/ PGPR3    |
| 35   | C5* PGPR1/PGPR2/ PGPR3   |

**3. Elicitor**

| ID | Treatment | 
| -------- | -------- | 
| 1     | C1* E1    |
| 2    | C2* E1   |
| 3    | C3* E1    |
| 4   | C4* E1   |
| 5    | C5* E1    |
| 6     | C1* E2    |
| 7    | C2* E2   |
| 8    | C3* E2    |
| 9   | C4* E2   |
| 10    | C5* E2    |
| 11     | C1* E1/ E2    |
| 12    | C2* E1/ E2   |
| 13    | C3* E1/ E2    |
| 14   | C4* E1/ E2   |
| 15    | C5* E1/ E2    |

**4. Year**

| Name | Year | Other information |
| -------- | -------- | -------- |
| First     | 1 year    | Labortory work     |
| Second    | 6 months    | Field experiment (1) greenhouse    |
| Third     | 6 months     | Field experiment (2) greenhouse    |
|     | ?     | Gene expression/ RNA sequencing     |


