# Plant Microbe Interaction :bug:
###### tags: 2022/02/28~2022/03/04, group project

## Tomato late blight (Phytophthora infestans)


## <font color=Blue> Background information </font>

**1. Cultivars**

| Cultivar | Gene | Other information |
| -------- | -------- | -------- |
| *Solanum lycopersicum* | | commercial variety |
| *Solanum pimpinellifolium* | ph1, ph2, ph3, ph4, ph5 | wild type |

**2. Strains pathogens to focus on**

| Name | Other information |
| -------- | -------- |
| US20 | |
| US21 | |
| US22 | |
| US23 | |

**3. 3 PGPR**

| ID | Name | Efficiency (green house) |
| -------- | -------- | -----|
| PGPR1 | *Paenibacillus polymyxa AB15* |74% |
| PGPR2 | *Bacillus pumilus SE34* | 42%|
| PGPR3 | *Pseudomonas fluorescens SS101* |80% |

**4. 2 Elicitor**

| ID | Name | Efficiency |
| -------- | -------- | -------- |
| E1 | Chitosan |Reduce in vitro mycelium growth at 78% |
| E2 | Algal | Reduce 36% disease severity of *Sargassum fusiforme* |

------------

## <font color=blue> Protocol </font>

**1. 5 Cultivars**

| Name | Gene | Cultivar | Company| Other infor mation |
| -------- | -------- | -------- |-------- |-------- |
| C1 | ph2/ph3 | Pruden's Purple |Johnny’s selected seed |
| C2 | ph3 | Plum Regal | Johnny’s selected seed | ph3 is the major gene|
| C3 | ph2 | *Solanum lycopersicum moboline* | Erptomato | T1|
| C4 | ph1, ph2, ph3, ph4, ph5 | *Solanum pimpinellifolium* | scientists | might have the best result|
| C5 (control) | Nothing | *Solanum lycopersicum var. Rumba* | farmers | |

**2. 35 PGPRs**

| ID | Treatment |
| -------- | -------- |
| 1 | C1* PGPR1 |
| 2 | C2* PGPR1 |
| 3 | C3* PGPR1 |
| 4 | C4* PGPR1 |
| 5 | C5* PGPR1 |
| 6 | C1* PGPR2 |
| 7 | C2* PGPR2 |
| 8 | C3* PGPR2 |
| 9 | C4* PGPR2 |
| 10 | C5* PGPR2 |
| 11 | C1* PGPR3 |
| 12 | C2* PGPR3 |
| 13 | C3* PGPR3 |
| 14 | C4* PGPR3 |
| 15 | C5* PGPR3 |
| 16 | C1* PGPR1/ PGPR2 |
| 17 | C2* PGPR1/ PGPR2 |
| 18 | C3* PGPR1/ PGPR2 |
| 19 | C4* PGPR1/ PGPR2 |
| 20 | C5* PGPR1/ PGPR2 |
| 21 | C1* PGPR1/ PGPR3 |
| 22 | C2* PGPR1/ PGPR3 |
| 23 | C3* PGPR1/ PGPR3 |
| 24 | C4* PGPR1/ PGPR3 |
| 25 | C5* PGPR1/ PGPR3 |
| 26 | C1* PGPR2/ PGPR3 |
| 27 | C2* PGPR2/ PGPR3 |
| 28 | C3* PGPR2/ PGPR3 |
| 29 | C4* PGPR2/ PGPR3 |
| 30 | C5* PGPR2/ PGPR3 |
| 31 | C1* PGPR1/PGPR2/ PGPR3 |
| 32 | C2* PGPR1/PGPR2/ PGPR3 |
| 33 | C3* PGPR1/PGPR2/ PGPR3 |
| 34 | C4* PGPR1/PGPR2/ PGPR3 |
| 35 | C5* PGPR1/PGPR2/ PGPR3 |

**3. Elicitor**

| ID | Treatment |
| -------- | -------- |
| 1 | C1* E1 |
| 2 | C2* E1 |
| 3 | C3* E1 |
| 4 | C4* E1 |
| 5 | C5* E1 |
| 6 | C1* E2 |
| 7 | C2* E2 |
| 8 | C3* E2 |
| 9 | C4* E2 |
| 10 | C5* E2 |
| 11 | C1* E1/ E2 |
| 12 | C2* E1/ E2 |
| 13 | C3* E1/ E2 |
| 14 | C4* E1/ E2 |
| 15 | C5* E1/ E2 |

**4. Year**

| Name | Year | Other information |
| -------- | -------- | -------- |
| First | 1 year | Labortory work |
| Second | 6 months | Field experiment (1) greenhouse |
| Third | 6 months | Field experiment (2) greenhouse |
| | ? | Gene expression/ RNA sequencing |