<!-- .slide: data-background="#1A237E" --> ## Singlet oxygen production and detection Iveta Terezie Pelikánová ---- - First year PhD student - Departement of Nuclear Chemistry, Faculty of Nuclear Sciences and Physical Engineering, CTU - Institute of Physics of the Czech Academy of Sciences --- ## Motivation for studying ^1^O~2~ ---- Antibacterial effect Photodynami therapy - skin diseases,cancer ---- Princip of Photodynamic Therapy - photosensitizer - visible light - molecule oxygen in tissue -> cell damage or cell death ----  ----  --- ## Singlet oxygen ---- - diatomic molecule oxygen - reactivity vs. spin prohibition  ----  --- ## Singlet oxygen prodution ---- - during chemical reactions - photochemical production with photosensitizers (PS)  ---- Photosensitizers - merocyanine 540 - rose bengal - methylene blue - photoporhyrine IX ---- Merocyanine 540  ---- Rose Bengal  ---- Methylene Blue  ---- Porphyrine IX (A) and Heme (B)  --- ## PDT induced by X-rays (PDTX) ----  --- ## Scintillators for PDTX ---- - high light yield of scintillator - emission spectrum of scintillator overlay absorption spectrum of photosensitizer - low toxicity - ZnO:Ga - LuAG:Pr^3+^ ---- ZnO:Ga, LuAG:Pr^3+^   --- ## Singlet oxygen detection ---- Direct x Indirect --- ## Fluorescence chemical detection ---- Chemical probes - Aminophenyl fluorescein (APF) - Singlet Oxygen Sensor Green (SOSG) ---- APF  ---- SOSG  ---- Singlet oxygen quencher NaN~3~  ---- NaN~3~ effects absorption spectra of SOSG ----  --- # Thank you