---
title: "Supplemental Information"
author: "Parris T Humphrey"
---

## Supplemental Information

<br>

### Supplemental Figures

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<center><img src="https://i.imgur.com/nx1AZMl.png" width = "600"></center><br>

><strong>Fig. S1. Growth cycle profiles of 40 *Pseudomonas* spp. strains measured individually in minimal media <em>in vitro</em></strong>. Overlain individual growth curves are shown grouped by nearest phylogenetic neighboring strains (see Fig. 1, main text). Strain 08B is sister to *P. syringae* clade. 4326 = laboratory strain *P. syringae* pv. maculicola str. ES4326.

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<center><img src="https://i.imgur.com/tQIzT4e.png" width = "450"></center><br>

><strong>Fig. S2.</strong> <strong>Life history trait correlations exhibited by *P. syringae* and *P. fluorescens* strains.</strong> Slopes of clade-wise linear regression are over-plotted in each panel for illustration purposes only regardless of $p$ value of correlation coefficient (see Fig. 1c, main text).

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<center><img src="https://i.imgur.com/vwxokxu.png" width = "600"></center><br>

><strong>Fig. S3.</strong> <strong>Correlations among competitive traits exhibited by *P. syringae* and *P. fluorescens* strains.</strong> Note that $C_{t}$ for *P. syringae* are all 0 because none produced detectable inhibitors scored in our pairwise soft-agar competition assays (see Extended Data Fig. 1, main text). Slopes of clade-wise linear regression are over-plotted in each panel for illustration purposes only regardless of $p$ value of correlation coefficient (see Fig. 1c, main text).
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<center><img src="https://i.imgur.com/8OZeMFy.png" width = "400"></center><br>

><strong>Fig. S4.</strong> <strong>Correlations between competitive traits and life-history traits exhibited by *P. syringae* and *P. fluorescens* strains.</strong> Slopes of clade-wise linear regression are over-plotted in each panel for illustration purposes only regardless of $p$ value of correlation coefficient (see Fig. 1c, main text).

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<center><img src="https://i.imgur.com/iu8Mulc.png" width = "200"></center><br>

><strong>Fig. S5.</strong><strong> Multivariate trait dispersion within <i>P. syringae</i> and <i>P. fluorescens</i> clades</strong>. Pairwise Euclidean distances along trait PCs 1-3 are greater for <i>P. syringae</i> strains than among <i>P. fluorescence</i> strains (unequal variance two-sample [Welch's] $t$-test, $t = 8.71; p < 10^{-5}$).

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<hr>

## Supplemental Tables

### Table S1. Summary statistics of life history and competitive trait distributions between *Pseudomonas* spp. clades.

#### Summary statistics of life history and competitive trait distributions.

<small>
<table class="table table-condensed" style="margin-left: auto; margin-right: auto;">
<thead>
<tr>
<th style="text-align:left;"> trait </th>
<th style="text-align:left;"> <i>P. fluorescens</i> </th>
<th style="text-align:left;"> <i>P. syringae</i> </th>
</tr>
</thead>
<tbody>
<tr grouplength="9"><td colspan="3" style="border-bottom: 1px solid;"><strong>mean (stdev)</strong></td></tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>r</i> </td>
<td style="text-align:left;"> 0.57 (0.15) </td>
<td style="text-align:left;"> 0.34 (0.22) </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>L</i> </td>
<td style="text-align:left;"> 672.13 (216.43) </td>
<td style="text-align:left;"> 1549.86 (656.52) </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>K</i> </td>
<td style="text-align:left;"> 0.49 (0.06) </td>
<td style="text-align:left;"> 0.42 (0.18) </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>i_w</i> </td>
<td style="text-align:left;"> 0.15 (0.19) </td>
<td style="text-align:left;"> -0.28 (0.22) </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>c_w</i> </td>
<td style="text-align:left;"> 0.65 (0.2) </td>
<td style="text-align:left;"> -0.35 (0.3) </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>c_t</i> </td>
<td style="text-align:left;"> 0.18 (0.16) </td>
<td style="text-align:left;"> 0 (0) </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>c_r</i> </td>
<td style="text-align:left;"> 0.97 (0.05) </td>
<td style="text-align:left;"> 0.72 (0.22) </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>c_o</i> </td>
<td style="text-align:left;"> 0.82 (0.1) </td>
<td style="text-align:left;"> 0.26 (0.13) </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>c_d</i> </td>
<td style="text-align:left;"> 0.83 (0.11) </td>
<td style="text-align:left;"> 0.39 (0.2) </td>
</tr>
<tr grouplength="9"><td colspan="3" style="border-bottom: 1px solid;"><strong>[min; max]</strong></td></tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>r</i> </td>
<td style="text-align:left;"> [0.41; 0.91] </td>
<td style="text-align:left;"> [0.03; 1.09] </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>L</i> </td>
<td style="text-align:left;"> [446.1; 1126.92] </td>
<td style="text-align:left;"> [470.52; 2716.58] </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>K</i> </td>
<td style="text-align:left;"> [0.36; 0.57] </td>
<td style="text-align:left;"> [0.03; 0.71] </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>i_w</i> </td>
<td style="text-align:left;"> [-0.11; 0.56] </td>
<td style="text-align:left;"> [-0.77; 0] </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>c_w</i> </td>
<td style="text-align:left;"> [0.3; 0.88] </td>
<td style="text-align:left;"> [-0.85; 0.31] </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>c_t</i> </td>
<td style="text-align:left;"> [0; 0.56] </td>
<td style="text-align:left;"> [0; 0] </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>c_r</i> </td>
<td style="text-align:left;"> [0.83; 1] </td>
<td style="text-align:left;"> [0.23; 1] </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>c_o</i> </td>
<td style="text-align:left;"> [0.64; 0.94] </td>
<td style="text-align:left;"> [0.04; 0.67] </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> <i>c_d</i> </td>
<td style="text-align:left;"> [0.65; 1] </td>
<td style="text-align:left;"> [0.03; 0.64] </td>
</tr>
</tbody>
<tfoot>
<tr>
<td style = 'padding: 0; border:0;' colspan='100%'>^a <i>r</i> displayed as x 1000</td>
</tr>
</tfoot>
</table>
</small>

<br>

### Table S2. Distribution of outcomes by pairing type.

<small>
<table class="table table-condensed" style="width: auto !important; margin-left: auto; margin-right: auto;">
<thead>
<tr>
<th style="text-align:left;"> predictor_col </th>
<th style="text-align:right;"> ASYM </th>
<th style="text-align:right;"> RI </th>
<th style="text-align:right;"> RNI </th>
</tr>
</thead>
<tbody>
<tr grouplength="3"><td colspan="4" style="border-bottom: 1px solid; text-align:left"><strong>Counts</strong></td></tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> Between </td>
<td style="text-align:right;"> 353 </td>
<td style="text-align:right;"> 2 </td>
<td style="text-align:right;"> 2 </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> Within (<i>P. fluorescens</i>) </td>
<td style="text-align:right;"> 72 </td>
<td style="text-align:right;"> 16 </td>
<td style="text-align:right;"> 3 </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> Within (<i>P. syringae</i>) </td>
<td style="text-align:right;"> 259 </td>
<td style="text-align:right;"> 20</td>
<td style="text-align:right;"> 45 </td>
</tr>
<tr grouplength="3"><td colspan="4" style="border-bottom: 1px solid; text-align:left"><strong>Frequencies</strong></td></tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> Between </td>
<td style="text-align:right;"> 0.989 </td>
<td style="text-align:right;"> 0.006 </td>
<td style="text-align:right;"> 0.006 </td>
</tr>
<tr>
<td style="text-align:left; padding-left: 2em;" indentlevel="1"> Within (<i>P. fluorescens</i>) </td>
<td style="text-align:right;"> 0.79 </td>
<td style="text-align:right;"> 0.18 </td>
<td style="text-align:right;"> 0.03 </td>
</tr>
<tr>
<td style="padding-left: 2em;" indentlevel="1"> Within (<i>P. syringae</i>) </td>
<td style="text-align:right;"> 0.80 </td>
<td style="text-align:right;"> 0.06 </td>
<td style="text-align:right;"> 0.14 </td>
</tr>
</tbody>
<tfoot>
<tr>
<td style = "text-align:right; padding: 0; border:0;" colspan='100%'>^a RNI = Reciprocal non-invasion</td>
</tr>
<tr>
<td style = 'padding: 0; border:0;' colspan='100%'>^b RI = Reciprocal invasion</td>
</tr>
<tr>
<td style = 'padding: 0; border:0;' colspan='100%'>^c ASYM = Asymmetric dominance</td>
</tr>
</tfoot>
</table>
</small>
<br>

### Table S3. Multinomial model coefficients.

<small>
<table class="table table-condensed" style="width: auto !important; margin-left: auto; margin-right: auto;">
<thead>
<tr>
<th style="text-align:left;"> outcome </th>
<th style="text-align:left;"> term </th>
<th style="text-align:right;"> coefficient </th>
<th style="text-align:right;"> std.err </th>
<th style="text-align:right;"> <i>z</i> </th>
<th style="text-align:right;"> <i>p</i> </th>
</tr>
</thead>
<tbody>
<tr>
<td style="text-align:left;"> RI </td>
<td style="text-align:left;"> intercept </td>
<td style="text-align:right;"> 0.01 </td>
<td style="text-align:right;"> 0.71 </td>
<td style="text-align:right;"> -7.30 </td>
<td style="text-align:right;"> 0.0000 </td>
</tr>
<tr>
<td style="text-align:left;"> RI </td>
<td style="text-align:left;"> <i>P. fluorescens</i> </td>
<td style="text-align:right;"> 39.22 </td>
<td style="text-align:right;"> 0.76 </td>
<td style="text-align:right;"> 4.82 </td>
<td style="text-align:right;"> 0.0000 </td>
</tr>
<tr>
<td style="text-align:left;"> RI </td>
<td style="text-align:left;"> <i>P. syringae</i> </td>
<td style="text-align:right;"> 13.63 </td>
<td style="text-align:right;"> 0.75 </td>
<td style="text-align:right;"> 3.50 </td>
<td style="text-align:right;"> 0.0005 </td>
</tr>
<tr>
<td style="text-align:left;"> RNI </td>
<td style="text-align:left;"> intercept </td>
<td style="text-align:right;"> 0.01 </td>
<td style="text-align:right;"> 0.71 </td>
<td style="text-align:right;"> -7.30 </td>
<td style="text-align:right;"> 0.0000 </td>
</tr>
<tr>
<td style="text-align:left;"> RNI </td>
<td style="text-align:left;"> <i>P. fluorescens</i> </td>
<td style="text-align:right;"> 7.36 </td>
<td style="text-align:right;"> 0.92 </td>
<td style="text-align:right;"> 2.16 </td>
<td style="text-align:right;"> 0.0305 </td>
</tr>
<tr>
<td style="text-align:left;"> RNI </td>
<td style="text-align:left;"> <i>P. syringae</i> </td>
<td style="text-align:right;"> 30.67 </td>
<td style="text-align:right;"> 0.73 </td>
<td style="text-align:right;"> 4.71 </td>
<td style="text-align:right;"> 0.0000 </td>
</tr>
</tbody>
<tfoot>
<tr>
<td style = 'padding: 0; border:0;' colspan='100%'>^a RNI = Reciprocal non-invasion</td>
</tr>
<tr>
<td style = 'padding: 0; border:0;' colspan='100%'>^b RI = Reciprocal invasion</td>
</tr>
<tr>
<td style = 'padding: 0; border:0;' colspan='100%'>^c coefficients = odds</td>
</tr>
</tfoot>
</table>
</small>
<br>

### Table S4. Linear regression of trait distance versus phylogenetic distance by clade.

<small>
<table class="table table-striped table-hover table-condensed" style="margin-left: auto; margin-right: auto;">
<thead>
<tr>
<th style="text-align:left;"> term </th>
<th style="text-align:right;"> estimate </th>
<th style="text-align:right;"> std.error </th>
<th style="text-align:right;"> statistic </th>
<th style="text-align:right;"> p.value </th>
</tr>
</thead>
<tbody>
<tr>
<td style="text-align:left;"> intercept (Pflu) </td>
<td style="text-align:right;"> 1.03 </td>
<td style="text-align:right;"> 0.17 </td>
<td style="text-align:right;"> 6.22 </td>
<td style="text-align:right;"> <10^-9 </td>
</tr>
<tr>
<td style="text-align:left;"> phylo-dist </td>
<td style="text-align:right;"> 0.09 </td>
<td style="text-align:right;"> 0.01 </td>
<td style="text-align:right;"> 6.46 </td>
<td style="text-align:right;"> <10^-10 </td>
</tr>
<tr>
<td style="text-align:left;"> Psyr </td>
<td style="text-align:right;"> 0.88 </td>
<td style="text-align:right;"> 0.15 </td>
<td style="text-align:right;"> 5.76 </td>
<td style="text-align:right;"> <10^-8 </td>
</tr>
</tbody>
</table>
</small>