# Walkthrough notes ## Chris - [X] add intro slides - [X] explain how we see microbes - [X] update slides on Github - [X] explain artifact = zip file - [X] amplicon sequencing variants - [X] make jumps to the notebooks - [X] trees before diversity part - [x] mention data limitations (trimming) - [X] project challenge? - [x] double check and adjust subsistence ## Nick - [X] flux direction is usually inferred from free energy (pH, temp) - [X] constant flow = can run forever - [X] annotate flux image - [X] more general unique solution fluxes - [X] maybe only simulate one diet and have the other one as exercise - [X] label none as no L2 - [X] add legend to hairball - [X] draw strategy for medium - [X] fix T-SNE defaults in micom - [X] unique growth rates - [X] most taxa - [X] update AGORA # Notebooks ## 16S - [X] include representation slide - [X] update exercise # 20221007 - [ ] change functional slide from sequencing -> omics - [ ] no need to apologize :wink: - [X] *Chris:* make the control buttons responsive with the side bar - [ ] no need to explain all reduction methods, just mention the general idea - [X] *Chris*: fix the media and don't touch them again before the course - [ ] use `save_results`, `load_results` in the notebooks