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# System prepended metadata

title: Metagenomic analysis of Indian Ocean bee data

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# Metagenomic analysis of Indian Ocean bee data 
You are provided with 16s bees metagenomic data from an Indian Ocean Study. In this project, we aim to address the following questions:

1. Is there any effect of location? (Keep in mind that Comoros and Zanzibar samples were whole abdomen bees and not dissected guys, and this is where variation might come)
2. Or does the difference come from stochastic variability among individual samples? In other words, is there a signature of hive or apiary or not at all?
3. Are there variations among island islands (Seychelles, Mauritius, Zanzibar have many islands)? In other words, is the difference by country or by island? A sub-question of this point will be is Rodriguez different to the rest? (They have a different bee subspecies)
4. Is there any relation between pathogens find in those islands and the gut microbiota composition? (See table attached). Although the samples where pathogens were measured were not collected at the same time.
5. Any other particularity you find will be great. I don't know if you want to explore as well some phylogeny or comparison with other studies samples.

In addition to analysing the data, this study aims to convert the 16s rRNA pipelines to Nextflow language. 

