Sticky exercise: Let's bring it back out to the questions that brought you here to STAMPS! Please populate your stickies (yellow and pink) as follows: ## Yellow/Green Stickies: Scientific Questions - **"Questions you'd like to ask of microbial communities."** Please try to abstract/generalize! - _who is there?_ - _what are they doing?_ ## Pink Stickies: Tools - **"Tools for analyzing microbial sequence data"** - _sourmash_ - ...? ## Part 2: Build a Framework! - How would you link the tools answer these questions? **Can you generalize the _process_ being used at each step**? - What steps are in common; what steps are unique? - Are there critical missing links? ## Part 3: Discuss other Frameworks - What did they do differently? - Do you agree/disagree on anything? ## Part 4: Recap ## Closing thoughts - Your questions are always the most important part. - All these tools (wet & dry lab) are imperfect ways to investigate microbial organisms and communities. - Methods will change over time (hopefully for the better). - Recognize their limitations, but don't let that hold back your science.