---
# System prepended metadata

title: Golden Gate Demo 2

---

# Golden Gate Demo 2

**Date:** 10/06/2022

## Present
- Kevin
- James
- Delphine
- Louis (Eyckerman group)
- 

## Agenda

* Welcome
* Get everyone to browse to https://gg2-assembler-test.vib.be
* ...

### Demo

* Ask user to create a Level 0 (Demo by Kevin)
* Ask user to create a backbone (by user)
* Ask user to create a Level 1 (by user)
* Download Genbank files of Level 1 they just created (by user)

INSERT INTO users_vector_mapping ("user", "vector") SELECT 2, id FROM vectors;

### Remarks

- Extra features:
    - Application should check level0 and backbones for >2 BsaI sites. If that's the case: The user should get an error.
    - Check level0 for > 1 BsmB1 site. If that's the case: The user should get an error.
    - Extra radiobutton "ALL" in radiorow of overhangs
    - In addition to the standard set of overhangs, all other possible overhangs should be available as non-standard overhangs.
    - Show a legend of the construct:

> Level2 : [color in visualisation] [Location] - [Name]
> - Level1 : [color in visualisation] [Bsmb1 overhang] [Location] - [Name]
    > - Level0 : [color in visualisation] [Bsa1 overhang] [Location] - [Name]
    > - Level0 : [color in visualisation] [Bsa1 overhang] [Location] - [Name]
    > - Level0 : [color in visualisation] [Bsa1 overhang] [Location] - [Name]
    > - Level0 : [color in visualisation] [Bsa1 overhang] [Location] - [Name]
    > - Level0 : [color in visualisation] [Bsa1 overhang] [Location] - [Name]
    > - Level0 : [color in visualisation] [Bsa1 overhang] [Location] - [Name]
    > - Backbone (for Level1) : [color in visualisation] [Bsa1 overhang] [Bsmb1 overhang] [Location] - [Name]
> - Level1 : [color in visualisation] [Bsmb1 overhang] [Location] - [Name]
> - Backbone (for Level2) :[color in visualisation] [Bsmb1 overhang] [Location] - [Name]
    - Validation of the construct before adding it to the database.
