# Golden Gate Demo 2 **Date:** 10/06/2022 ## Present - Kevin - James - Delphine - Louis (Eyckerman group) - ## Agenda * Welcome * Get everyone to browse to https://gg2-assembler-test.vib.be * ... ### Demo * Ask user to create a Level 0 (Demo by Kevin) * Ask user to create a backbone (by user) * Ask user to create a Level 1 (by user) * Download Genbank files of Level 1 they just created (by user) INSERT INTO users_vector_mapping ("user", "vector") SELECT 2, id FROM vectors; ### Remarks - Extra features: - Application should check level0 and backbones for >2 BsaI sites. If that's the case: The user should get an error. - Check level0 for > 1 BsmB1 site. If that's the case: The user should get an error. - Extra radiobutton "ALL" in radiorow of overhangs - In addition to the standard set of overhangs, all other possible overhangs should be available as non-standard overhangs. - Show a legend of the construct: > Level2 : [color in visualisation] [Location] - [Name] > - Level1 : [color in visualisation] [Bsmb1 overhang] [Location] - [Name] > - Level0 : [color in visualisation] [Bsa1 overhang] [Location] - [Name] > - Level0 : [color in visualisation] [Bsa1 overhang] [Location] - [Name] > - Level0 : [color in visualisation] [Bsa1 overhang] [Location] - [Name] > - Level0 : [color in visualisation] [Bsa1 overhang] [Location] - [Name] > - Level0 : [color in visualisation] [Bsa1 overhang] [Location] - [Name] > - Level0 : [color in visualisation] [Bsa1 overhang] [Location] - [Name] > - Backbone (for Level1) : [color in visualisation] [Bsa1 overhang] [Bsmb1 overhang] [Location] - [Name] > - Level1 : [color in visualisation] [Bsmb1 overhang] [Location] - [Name] > - Backbone (for Level2) :[color in visualisation] [Bsmb1 overhang] [Location] - [Name] - Validation of the construct before adding it to the database.