# An Introduction to TAVNS Electrical Stimulation Programming in NI LabVIEW
## SOP
迷走神經耳電刺激操作流程（SOP）
一、 架設備
1. 開啟電腦電源。
2. 插入DAQ卡、USB卡後，開啟電源。
3. 開啟DS5之電源(屁股)，插入連接線(將電極依照正負極插入。
5. 開啟NI程式，鍵入受試者姓名、日期。

二、 準備實驗－受試者準備
1. 清潔受試者之左耳並卸下耳朵周圍無金屬飾品，如耳環、髮夾等。
2. 將電極片塗上適量導電膠。
3. 使用彈性繃帶將塗有導電膠的電極片黏貼於電刺激部位（注意正負極），並觀察及確認黏貼位置確實服貼。

三、 DS5儀器準備設定
1. 按下NI程式之run➪按鈕後執行Auto zero。
2. 開啟DS5之脈衝開關（上扳or下扳）。
3. 將NI程式之頁籤（tab）切換為Testing Mode，由50 μA為電流起始值，詢問受試者是否有感覺，若受試者無感覺，以50 μA為單位依序增加電流量，直到有感覺時，將此電流數值，設置於NI程式中As stim threshold。
4. 完成stim threshold之設置後，繼續增加電流強度，直到受試者感覺有點不適，其數值設置在NI程式中As max threshold。
5. 由stim threshold 及max threshold之平均值作為刺激強度並執行save amplitude（儲存此電流刺激之振福）。
6. NI程式中Testing Mode所需執行工作包含：確認stim threshold、max threshold、刺激強度（平均值），save amplitude皆完成後按done以結束Testing Mode。
7. 將NI程式之頁籤（tab）切換為Running Mode，讀取檔案？，再按下run➪按鈕。
8. 執行時間超過1530秒後可停止程式。

四、 收拾
1. 關閉DS5之脈衝開關（上扳or下扳）。
2. 關DAQ卡、DS5開關、電腦電源
3. 清潔電極（水洗）、自然風乾

## GUI
## Module code

## Setting

### step1

*If the computer is already connected to the DAQ device, this step can be skipped.*

* To accommodate various DAQ devices. This involves navigating to the 'Tools' menu and selecting 'Measurement & Automation Explorer' to access the necessary settings."

* To open Measurement & Automation Explorer, click on 'Device and Interface' on the left-hand side, then select 'Create New'.

* Verify the correct model of the DAQ before proceeding with the selection.

## Adjusting Functionality
### stimulation intensity
The stimulation intensity is primarily controlled by the module labeled 'Stimulation amplitude for testing (uA).
#### GUI
#### Module code

The default increment and decrement for stimulation intensity are set to **50uA**.
To adjust, right-click on the module and select 'Properties'.
The minimum and maximum range for stimulation intensity adjustment can also be modified in this section.
### stimulation time
The default mode is Testing mode, to switch to Running mode, navigate to the GUI's top menu.

To switch to Running mode, scroll down to find the module block.



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Due to the introduction of a new DAQ machine (model: 6221) in the hospital, the old program can still be used, but certain settings need to be adjusted. Otherwise, the following error will occur during execution.
After clicking 'stop,' it will navigate to the modular program page, Next, click on the DAQ-related module block.
Then, the current DAQ work window will open, and you need to remove the existing channels first.
Next, add the channels and select either 'ao0' or 'ai0.
**Follow the above steps to re-add the new channels for all DAQ blocks.**

The updated program for the new DAQ model has been completed and can be found on the laboratory NAS.