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# System prepended metadata

title: Experimental Protocol

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# Experimental Protocol

## Protocol to operate the Camera([Photron]  mini UX-50)

### Structure
* Tripod is fixed at the location from where we want to capture the images, there is the leveler in the tripod that must be ensured that, it is in the middle of the marking to make proper balancing.
* Mini UX-50 camera is then placed at the top of the tripod and it should be properly locked to avoid the falling from tripod.
* Tripod has many movement option like we can adjust angle between legs of tripod, We can change the height of the camera by nob rotation in the center of tripod,that  after adjusting the height shoud be tigtend properly. There is an option for rotation about verticle axis and other option about horizontal axis rotation, all we can adjust according to our requirement.
* There are many options for electrical connection from camera but currently we are using only two, one is the the power cord that gives the DC power supply from the camera adopter, while connecting the cord to camera it should be taken care of the the cord is havig red oipn and the camera female cord is also having red point during connectin bothe coincide and it should be easy pressure to make connection, we don't need to apply force to avoid breakge. Other connection is the data transmission cable( LAN Cable) which is conncted to camera and Computer.
* To operate the camera there is required a software for perticular comapany, here we installed PFV-4 software that is compatible with MINI UX-50.
### Operation 
* After following the all the above steps now we need to switch-on the power to adopter and then we need to on the camera by pressing the power button(White Colour button on the back side of the camera), it will start will mild noise seems like running fan for cooling and immidiatly it will stop.
* Now open the installed software(PFV-4)and set the IP address(192.168.0.1 or 192.168.0.15), it will ask the permission allow it and it will open the main interface where we can see so many open like LIVE, Memory and the right side we will see the option for the frame rate, resolution and shutter speed, dark mode.
* We have set the frame rate of camera at 4000fps, resolution at 640*480 and shutter speed at 1/256000.
* There is many option in the setting of the camera like partition saving the capture image ,calibration . Depeneding on our requirement we can change the number of partition like 1,2,3 or upto 25. As we will increase the number of partition the frame space will reduce so while capturing the image it will reduce the  image capturing time.
* Keeping the shutter speed as so high 1/256000, which will allow the shutter to close and open very fast, thus the light intensity will reduce inside the sensor of camera and it will prevantive action to avoid any potential damage  of the camera sensor because we don't  have apperture control within the camera.
* First of all we will switch on the LED light where we have already placed a ground glass in front of the LED light and we will open the cap of the camera slowly with the help of lens we will focus ath the region of our intrest. We need to capture the image of the scale at the point of focus(Jet axis)and will capture for the calibration of the image ie mm/pixel of any measuring unit/pixel.
* We will captute the distance where where is our region of intrest, for that we will place a scale at the nozzle exit in the same plane of liquid jet and capture it.
* We will choose partition 1 and capture the image of liquid jet and then we will switch to partition 2 and then we will go to live mode and we will press ready button it will be just before the image capturing stage, we will swithch on probe sonicator and capture for partition 2 and similar for other. 
* Experiment has been completed now close the Cap of the camera and switch off the LED light.
* Finally we need to save the all the image in the form of partion for that we will click on save so it will ask the location where we want to save the image and file name every time we will select the folder file name and file type(TIFF, JPEG, PNG)for best quality we will choose the TIFF.All the partion will be saved by just changing the file name.
* We have saved all the partition image now will close the PFV-4 software and we will close camera  remove all the connections detached from the tripod  and put it back safely to its casing   
## Experimental protocol 

### Precaution before starting  experiment 

* Check whether the N2 valve is close, that is connecting main line to pressure regulator.
* Check the pressure regulator is fully closed(i.e there is no gas coming at the oulet of the regulator ) and valve after pressure regulator should be closed.
* Check the valve that is connecting water pump to main pressure vessel. valve should be always closed.
* Check the valve that connect the pressurized liquid to glass rotameter it should be always closed.
* Check the glass rotameter knob that is tightly closed.
* Ensure that there is no leakage near the thread that connects nozzle to flage.
* Check the pressure release valve it should be closed.

## Steps to start the experiment

1) Ensure that all valves are closed
2) Open the pressure release valve( Pressure within pressure vessel will goes to ambient pressure[Zero Gauge Pressure]).
3) Close the Pressure release valve
4) Fill the bucket with the DI water and switch-on the pump to fill the pressure vessel, open the fluid intake valve so that fluid can enter from bucket to pressure vessel via pump.
5) After filling the tank close the intake valve and switch-on the motor.
6) Now start applying the pressure on the fluid with the help of N2 gas for that we have to open N2 entry valve near to pressure regulator.
7) Keep rotating the pressure reguator knob it will start passing gas through it and gauge will show its pressure reading. Set the knob according to your required pressure, now pressure within pressure vessel will be same as regulator outlet gauge shown pressure.
8) Ensure that nozzle is tightly connected and and is leakproof from the thread.
9) We have three set of working nozzle respective diameter in micrometer([1] Chemical Nozzle=,[2] Mechanical Nozzle=,[3] Bhrigunath Sameer sir lab=) that can be used one by one according to our requirement.
10) Set LED light and Camera facing to each other, Ground glass is used near to LED light to get continious diffused light, switch on the LED light.
11) Open the computer set all the connection for the NI-DAQ module, now open the NI-Max software beensure that DAQ is connected to chassis, for that we need to do a self testing od DAQ, It will show green mark if device connection is set.
12) Open the VI module for the pressure measurement, Pressure sensor is connected to DAQ that will give the electrical singal in the form of voltage.
13) Pressure sensor will give voltage singal upto 10 volt DC for the gauge pressure of 40 bar So Vi is modified, it will enhance the reading four time what the sensor will send. So exact pressure we will able to know. 
14) Open the valve that connect pressure vessel to glass rotameter and start rotating the rotameter knob to get desired LPM of your requirement. 
15) Jet will start from the nozzle exit, it will forms cylindtical jet and then it will start breaking into ligaments and droplets.
16) We need to place clear transparent scale along and perpendicular axis of the jet, scle will the same axis of the jet and at the focus point of the camera, We need to take the snapshot of sacle in both axis for calibration(Parpendicual axis of the jet)and parallel axis of the jet to know the region from where to where camera is focus to jet.
17) Intially pressure is set to 1 bar, we will fisrt capture jet without any acoustic perturbation then we will switch to acoustic perturbation by(25,50,75 and 100%) and capture the jet droplet image again we will increase pressure to 2 bar and allow jet to be stable and capture the jet image without acoustic and with acoustic at different % this process will be continue till 8 bar. All the recorded image is saved from camera memory to computer.
18) Close all the valve, close the PFV-4 software and press camera off button, close the NI-DAQ sofware and hardware, close the pressure inlet valve and open the release valve to release the gas.

