# ChIP-Seq Pipeline summary 1. Raw read QC (FastQC) 2. Adapter trimming (Trim Galore!) 3. Alignment (BWA) 4. Mark duplicates (picard) 5. Merge alignments from multiple libraries of the same sample (picard) 1. Re-mark duplicates (picard) 2. Filtering to remove: reads mapping to blacklisted regions (SAMtools, BEDTools) reads that are marked as duplicates (SAMtools) reads that arent marked as primary alignments (SAMtools) reads that are unmapped (SAMtools) reads that map to multiple locations (SAMtools) reads containing > 4 mismatches (BAMTools) reads that have an insert size > 2kb (BAMTools) reads that map to different chromosomes (Pysam) reads that arent in FR orientation (Pyssam) reads where only one read of the pair fails the above criteria (Pysam) 6. Alignment-level QC and estimation of library complexity (picard, Preseq)\ 7. Create normalised bigWig files scaled to 1 million mapped reads (BEDTools, bedGraphToBigWig) 8. Generate gene-body meta-profile from bigWig files (deepTools) 9. Calculate genome-wide IP enrichment relative to control (deepTools) 10. Calculate strand cross-correlation peak and ChIP-seq quality measures including NSC and RSC (phantompeakqualtools) 11. Call broad/narrow peaks (MACS2) ## Multi-QC explanation https://nf-co.re/chipseq/1.2.1/output ## Phantompeakqualtools Calculate strand cross-correlation peak and ChIP-seq quality measures ### Publications https://pubmed.ncbi.nlm.nih.gov/22955991/ https://www.nature.com/articles/nbt.1508 ### cross-correlation peak strandshift figures ```bash ./bwa/mergedLibrary/phantompeakqualtools/3xHA-HD-like_R3.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/HD-like-3xHA_R1.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/NFYB3-3xHA_R3.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/NFYB3-3xHA_R2.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/NFYB3-3xHA_R1.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/3xHA_R3.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/3xHA-HD-like_R2.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/3xHA-MYB59_R3.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/3xHA_R2.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/3xHA-NFYB3_R2.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/HD-like-3xHA_R3.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/3xHA-NFYB3_R1.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/3xHA-HD-like_R1.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/MYB59-3xHA_R2.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/3xHA-MYB59_R2.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/3xHA_R1.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/3xHA-NFYB3_R3.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/MYB59-3xHA_R3.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/HD-like-3xHA_R2.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/MYB59-3xHA_R1.spp.pdf ./bwa/mergedLibrary/phantompeakqualtools/3xHA-MYB59_R1.spp.pdf ``` ## Picard Mark duplicate; Merge alignments from multiple libraries of the same sample ### Introduction https://www.broadinstitute.org/files/shared/mpg/plathumgen/plathumgen_fennell.pdf ### Citation “Picard Toolkit.” 2018. Broad Institute, GitHub Repository. http://broadinstitute.github.io/picard/; Broad Institute ### QC and metic plots ```bash ./bwa/mergedLibrary/picard_metrics/pdf/HD-like-3xHA_R1.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-MYB59_R1.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/MYB59-3xHA_R1.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA_R3.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-MYB59_R3.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-MYB59_R2.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/HD-like-3xHA_R2.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/HD-like-3xHA_R1.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA_R1.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-NFYB3_R1.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/MYB59-3xHA_R1.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/NFYB3-3xHA_R2.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/NFYB3-3xHA_R2.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-HD-like_R2.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/MYB59-3xHA_R3.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA_R2.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA_R2.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/MYB59-3xHA_R2.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-HD-like_R3.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-NFYB3_R1.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/HD-like-3xHA_R3.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA_R1.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-MYB59_R1.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA_R1.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/NFYB3-3xHA_R1.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/MYB59-3xHA_R2.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-MYB59_R3.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/HD-like-3xHA_R2.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA_R3.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-MYB59_R1.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/MYB59-3xHA_R2.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/NFYB3-3xHA_R2.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/HD-like-3xHA_R2.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/MYB59-3xHA_R3.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-NFYB3_R2.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/HD-like-3xHA_R1.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-NFYB3_R2.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-MYB59_R2.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/MYB59-3xHA_R3.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-HD-like_R2.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/MYB59-3xHA_R3.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/NFYB3-3xHA_R1.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA_R3.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/NFYB3-3xHA_R3.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-HD-like_R1.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-HD-like_R1.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA_R2.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/NFYB3-3xHA_R1.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-NFYB3_R3.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/HD-like-3xHA_R1.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-MYB59_R3.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/HD-like-3xHA_R3.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-HD-like_R1.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/HD-like-3xHA_R3.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/NFYB3-3xHA_R1.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-MYB59_R1.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/HD-like-3xHA_R3.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-NFYB3_R1.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-HD-like_R3.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-HD-like_R3.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-NFYB3_R3.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-MYB59_R2.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-HD-like_R3.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-NFYB3_R2.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-HD-like_R2.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/MYB59-3xHA_R2.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-HD-like_R1.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA_R1.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/MYB59-3xHA_R1.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-MYB59_R2.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-NFYB3_R3.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/HD-like-3xHA_R2.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-MYB59_R3.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA_R3.mLb.clN.CollectMultipleMetrics.quality_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/NFYB3-3xHA_R2.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-HD-like_R2.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-NFYB3_R3.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-NFYB3_R2.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/MYB59-3xHA_R1.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA_R2.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/NFYB3-3xHA_R3.mLb.clN.CollectMultipleMetrics.quality_distribution.pdf ./bwa/mergedLibrary/picard_metrics/pdf/NFYB3-3xHA_R3.mLb.clN.CollectMultipleMetrics.insert_size_histogram.pdf ./bwa/mergedLibrary/picard_metrics/pdf/NFYB3-3xHA_R3.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ./bwa/mergedLibrary/picard_metrics/pdf/3xHA-NFYB3_R1.mLb.clN.CollectMultipleMetrics.base_distribution_by_cycle.pdf ``` ## Deeptools Sequence coverage calculations ### Publications https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4086134/ ### Tutorials https://deeptools.readthedocs.io/en/develop/ ### Plots heatmap (due to low coverage, didn't work well) ```bash ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-NFYB3_R1.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA_R3.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-MYB59_R1.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/HD-like-3xHA_R3.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-HD-like_R3.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/HD-like-3xHA_R2.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/HD-like-3xHA_R3.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA_R1.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/MYB59-3xHA_R2.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-MYB59_R3.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/MYB59-3xHA_R3.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-MYB59_R2.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/NFYB3-3xHA_R1.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/MYB59-3xHA_R2.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/NFYB3-3xHA_R3.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/HD-like-3xHA_R2.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-HD-like_R3.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/HD-like-3xHA_R1.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/MYB59-3xHA_R3.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/NFYB3-3xHA_R3.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/MYB59-3xHA_R1.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/MYB59-3xHA_R1.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-HD-like_R2.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-NFYB3_R3.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-NFYB3_R3.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA_R2.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-HD-like_R2.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/NFYB3-3xHA_R2.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA_R3.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-HD-like_R1.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA_R1.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/NFYB3-3xHA_R2.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-HD-like_R1.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/NFYB3-3xHA_R1.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-NFYB3_R2.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-NFYB3_R2.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-MYB59_R3.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-MYB59_R2.plotProfile.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-NFYB3_R1.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/HD-like-3xHA_R1.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA-MYB59_R1.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotProfile/3xHA_R2.plotHeatmap.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/HD-like-3xHA_R3.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/3xHA-MYB59_R1.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/MYB59-3xHA_R1.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/MYB59-3xHA_R3.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/3xHA-NFYB3_R3.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/HD-like-3xHA_R1.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/3xHA-HD-like_R1.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/MYB59-3xHA_R2.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/HD-like-3xHA_R2.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/NFYB3-3xHA_R2.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/3xHA-NFYB3_R2.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/3xHA-HD-like_R3.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/3xHA-MYB59_R2.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/NFYB3-3xHA_R3.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/3xHA-MYB59_R3.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/NFYB3-3xHA_R1.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/3xHA-NFYB3_R1.plotFingerprint.pdf ./bwa/mergedLibrary/deepTools/plotFingerprint/3xHA-HD-like_R2.plotFingerprint.pdf ``` ## MACS2 Peak identifications ### Publications https://genomebiology.biomedcentral.com/articles/10.1186/gb-2008-9-9-r137 https://www.nature.com/articles/nprot.2012.101 ### Tutorials https://hbctraining.github.io/Intro-to-ChIPseq/lessons/05_peak_calling_macs.html ### MACS QC plots ```bash ./bwa/mergedLibrary/macs/broadPeak/qc/macs_peak.plots.pdf ./bwa/mergedLibrary/macs/broadPeak/qc/macs_annotatePeaks.plots.pdf ``` ### MACS intersect plots ```bash ./bwa/mergedLibrary/macs/broadPeak/consensus/MYB59/MYB59.consensus_peaks.boolean.intersect.plot.pdf ./bwa/mergedLibrary/macs/broadPeak/consensus/HDlike/HDlike.consensus_peaks.boolean.intersect.plot.pdf ./bwa/mergedLibrary/macs/broadPeak/consensus/NFYB3/NFYB3.consensus_peaks.boolean.intersect.plot.pdf ```
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