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Calibration Procedure

tags: respirometry, macmanes
title: Calibrating the FMS

To work gas tanks:

  • Open valve, connect tube and check flow rate before connecting to the FMS
  • flip uper left switch up so it is at baseline, calibrate system (see below)
  • Close tank, depressurize regulator for storage and turn off

Calibrate/Setting the water vapor span:

  • Before hooking any tubes up to the airtank, check that air and NOT water flowing through tubing
  • Flip the upper left switch to ‘baseline’ (instead of ‘chamber’) on FMS
  • Hook the a tube from FMS baseline (which is the really long tube) to the tank that contains no CO2 and let it run for 5 minutes.
    • You should see the water vapor values start to decrease towards zero.

How to check the accuracy of the upper H2O span:

  • Use the factory set upper limit
  • no action required

To zero the water vapor sensor

  • turn "mode dial" twice clockwise until you see "adjust zero"
  • then turn adjust dial (next to teh red one) to sent to zero.
  • hit enter (the red button below the water vapor display)

Calibrate/Setting O2 span:

  • You will need a tank of 21% O2, 0% CO2, the rest Nitrogen
  • You only need to set the upper span of this
  • Tank has already been connected gas from stap 1 above.
  • Flow needs to be at least 250, you should be able to feel air but it should not be "blasting"
  • Observe the O2 reading on the FMS (it might be wrong).
  • to chagne the value of 02, turn the "mode dial" 3 times until it reads "fixed span O2".
  • Turn the adjust dial until you get to 20.9%.
  • Hit enter.
  • done

Set CO2 zero using the current tank:

  • observe CO2 reading on gas analyzer (it should be zero but this is what we are adjusting)
  • turn "mode dial" to "zero CO2!"
  • hit enter
  • hit enter again to confirm. Display reads "hold please, this had better be CO2 free air"
  • done

Set CO2 1% using the other tank

  • You will need a tank of 1% CO2 balanced with air
  • Turn on the gas very low before hooking the tubes up to baseline
  • Connect to tank
  • wait a 3 minutes for things to stabalize.
  • turn "mode dial" to "Adjust CO2 span"
  • turn adjust until you are at 1%
  • hit red enter button.

change switch in upper left on FMS to computer

  • confirm that O2 reads about 21%
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