# Hackathon November 2020
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slides: https://hackmd.io/@Hackathon-November-2020/Hykb_fkcD
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# Project 4: RNA-seq analysis pipeline
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## Primary focus
Implement/modify a pipeline that performs RNA-seq analysis (proposals ranged from Ultra-low-input RNAseq to lncRNAs).
* Check https://nf-co.re/rnaseq/2.0
* Check https://github.com/nf-core/eager
* Check https://github.com/evanfloden/lncRNA-Annotation-nf
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## Activities
* Local setup of working environment (containers, data)
* Experiencing nf-core pipelines (download, structure, code)
* Experiencing nextflow (learned stuff & beyond, error correction)
* Tool installation within nextflow config
* Implementation RNAseq pipeline (Step 1-2)
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## People involved
- Frieder Hadlich (FBN)
- Wellison Diniz
- Lian Yu
- Praveen Chitneedi (FBN)
- Juan
- Marta Pozuelo
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## Github
https://github.com/friederhadlich/nextflow_ultraLowInput
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dataset
https://github.com/hartwigmedical/testdata/tree/master/100k_reads_hiseq/TESTX
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