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# System prepended metadata

title: Evotec Prep

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# Evotec Prep

## Interviewers
- Alexander Lomov
    - working in evotec since 8 years
    - started as associate now scientist 
    - background in BS molecular biology 

## Job description
- Research Associate (f/m/d) - Image and Data Analysis
    - Full-time and permanent

Your key responsibilities:

- Contribute to the application of imaging and analysis technologies as part of high content imaging for target validation, assay development and screening projects
- **Develop image analysis strategies and procedures, guide image acquisitions, microscopy work and subsequent data analysis**
- **Working in multi-disciplinary project teams**, you collaborate with scientists in various disease areas and provide expert knowledge to support experimental design
- Contribute to the **training of technical personnel** and maintenance of cutting-edge microscopy devices

Your ideal qualifications:

- MSc degree with several years of experience or a Ph.D degree in a Life Sciences area
- Profound knowledge with **image processing and data visualization, including handling of large and complex data sets** is necessary
- Ideally already first experience in developing analysis pipelines aiming to quantify and classify biological parameters extracted from microscopy data
- Basic programming skills are desirable
- Ability to work independently and in a well-organized manner, together with the initiative to actively contribute to the progress of our projects
- A concise analytical reasoning combined with a practical and creative approach to troubleshooting
- Excellent communication skills in a multidisciplinary environment
- Fluency in English, German would be an advantage

## Projects
- High content screening
    - FRET analysis of certain probes: used to study protein protein interactions
    - 2 proteins tagged with 2 fluorophore such that emission wavelength of 1 overlaps with excitation of other.
    - if the proteins are close by then we will see fret signal (<10 nm)
    - our proteins were tagged with cfp-yfp contsructs
        - if fret, we see yfp signal else only cfp
        - 96 well plate- 90 images per well --> 8640 images
        - camp sensor in cardiomyocites, low camp no fret high camp fret. Simulated with agents that increase or decrease camp -> study using fret
        - if proteins reduces camp -> we will not see fret
        - if protein increases camp -> we will see fret
        - fret efficiency **acceptor/donor**
    - camp or **Cyclic adenosine monophosphate** messenger, in cardiomyocites the regulation of cardiac myocyte contractile function
    - developed HCS workflow using cellprofiler
        - detect nuclei
        - detect cells using region growing
        - filter cells based on size , radius
        - create mask of only the cytoplasm region = cell - nucleus
        - measure intensities in acceptor, donor
        - compute fret efficiency
- Golgi body intensity over time
    - quantification of cargo transport in stimulated neuronal cells 
    - the golgi body accumulates signal - trend increasing curve from the point of stimulation
    - blue light stimulation 
    - live imaging, 2D+t with golgi marker
    - code to segment golgi and measure intensity over time

### Questions 

1. Current projects working on?
2. huge company and lots of teams and operations - where do you come in, explain flow of tasks in a project through various depts?
3. Where is the team, how many people?
4. Remote/hybrid 
5. 2025 goal
6. ways to grow??
7. How much % microscopy?

Notes

- 2 hcs units in hamburg, gottingen 
- 2025 goal

## Motivation 
- Love working with biological data and extracting info from them - always a challenge
- Evotec - always wanted to be part of a company, interaction with data scientists, focused work, work which has impact 
- why do you want to leave 
    - research - not permanent 
    - no opportunities for growth both personally and professionally 
- start - 3 months notice