# Evotec Prep

## Interviewers
- Alexander Lomov
- working in evotec since 8 years
- started as associate now scientist
- background in BS molecular biology

## Job description
- Research Associate (f/m/d) - Image and Data Analysis
- Full-time and permanent

Your key responsibilities:

- Contribute to the application of imaging and analysis technologies as part of high content imaging for target validation, assay development and screening projects
- **Develop image analysis strategies and procedures, guide image acquisitions, microscopy work and subsequent data analysis**
- **Working in multi-disciplinary project teams**, you collaborate with scientists in various disease areas and provide expert knowledge to support experimental design
- Contribute to the **training of technical personnel** and maintenance of cutting-edge microscopy devices

Your ideal qualifications:

- MSc degree with several years of experience or a Ph.D degree in a Life Sciences area
- Profound knowledge with **image processing and data visualization, including handling of large and complex data sets** is necessary
- Ideally already first experience in developing analysis pipelines aiming to quantify and classify biological parameters extracted from microscopy data
- Basic programming skills are desirable
- Ability to work independently and in a well-organized manner, together with the initiative to actively contribute to the progress of our projects
- A concise analytical reasoning combined with a practical and creative approach to troubleshooting
- Excellent communication skills in a multidisciplinary environment
- Fluency in English, German would be an advantage

## Projects
- High content screening
- FRET analysis of certain probes: used to study protein protein interactions
- 2 proteins tagged with 2 fluorophore such that emission wavelength of 1 overlaps with excitation of other.
- if the proteins are close by then we will see fret signal (<10 nm)
- our proteins were tagged with cfp-yfp contsructs
- if fret, we see yfp signal else only cfp
- 96 well plate- 90 images per well --> 8640 images
- camp sensor in cardiomyocites, low camp no fret high camp fret. Simulated with agents that increase or decrease camp -> study using fret
- if proteins reduces camp -> we will not see fret
- if protein increases camp -> we will see fret
- fret efficiency **acceptor/donor**
- camp or **Cyclic adenosine monophosphate** messenger, in cardiomyocites the regulation of cardiac myocyte contractile function
- developed HCS workflow using cellprofiler
- detect nuclei
- detect cells using region growing
- filter cells based on size , radius
- create mask of only the cytoplasm region = cell - nucleus
- measure intensities in acceptor, donor
- compute fret efficiency
- Golgi body intensity over time
- quantification of cargo transport in stimulated neuronal cells
- the golgi body accumulates signal - trend increasing curve from the point of stimulation
- blue light stimulation
- live imaging, 2D+t with golgi marker
- code to segment golgi and measure intensity over time

### Questions

1. Current projects working on?
2. huge company and lots of teams and operations - where do you come in, explain flow of tasks in a project through various depts?
3. Where is the team, how many people?
4. Remote/hybrid
5. 2025 goal
6. ways to grow??
7. How much % microscopy?

Notes

- 2 hcs units in hamburg, gottingen
- 2025 goal

## Motivation
- Love working with biological data and extracting info from them - always a challenge
- Evotec - always wanted to be part of a company, interaction with data scientists, focused work, work which has impact
- why do you want to leave
- research - not permanent
- no opportunities for growth both personally and professionally
- start - 3 months notice