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title: BRAILLE update (24-Feb-2021)
tags: [BRAILLE]

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tags: BRAILLE
title: BRAILLE update (24-Feb-2021)
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>**Interactive function plots and tables are [here](https://astrobiomike.shinyapps.io/braille-mg/)**.
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> Previous update docs
> - [https://hackmd.io/@astrobiomike/BRAILLE-update-3-Feb-2021](https://hackmd.io/@astrobiomike/BRAILLE-update-3-Feb-2021)
> - [https://hackmd.io/@astrobiomike/BRAILLE-notes-12-Dec-2020](https://hackmd.io/@astrobiomike/BRAILLE-notes-12-Dec-2020)

Metagenomic processing repository with data and code is at [OSF here](https://osf.io/uhk48/wiki/home/). All data are there, but not necessarily all of the processing and figures in these update docs.

# Taxonomy
Hadn't yet gotten to taxonomy (we did for MAGs, but not for the metagenome assemblies as a whole).

Clustering looks similar to the functional clustering:

<a href="https://i.imgur.com/mNFUM1v.png"><img src="https://i.imgur.com/mNFUM1v.png"></a>


<a href="https://i.imgur.com/pstjQCd.png"><img src="https://i.imgur.com/pstjQCd.png"></a>


<a href="https://i.imgur.com/Kj2jiKG.png"><img src="https://i.imgur.com/Kj2jiKG.png"></a>


<a href="https://i.imgur.com/GdtGqjZ.png"><img src="https://i.imgur.com/GdtGqjZ.png"></a>

Looks similar to [Wisechart et al. 2019](https://www.researchgate.net/publication/334083109_Shotgun_metagenomic_sequencing_from_Manao-Pee_cave_Thailand_reveals_insight_into_the_microbial_community_structure_and_its_metabolic_potential) from Manao-Pee cave in Thailand:

<a href="https://i.imgur.com/g5HS9Sa.png"><img src="https://i.imgur.com/g5HS9Sa.png"></a>


<a href="https://i.imgur.com/zhNKqiu.png"><img src="https://i.imgur.com/zhNKqiu.png"></a>


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Still working on main metabolism overview figures:

Nitrogen only one done so far

<a href="https://i.imgur.com/gnyEXPy.png"><img src="https://i.imgur.com/gnyEXPy.png"></a>

Planning Carbon and Sulfur too, maybe phosphorus