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title: BRAILLE update (17-Mar-2021)
tags: [BRAILLE]

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tags: BRAILLE
title: BRAILLE update (17-Mar-2021)
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>**Interactive function plots and tables are [here](https://astrobiomike.shinyapps.io/braille-mg/)**.
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# BRAILLE update (17-Mar-2021)

> Previous update docs
> - [https://hackmd.io/@astrobiomike/BRAILLE-update-24-Feb-2021](https://hackmd.io/@astrobiomike/BRAILLE-update-24-Feb-2021)
> - [https://hackmd.io/@astrobiomike/BRAILLE-update-3-Feb-2021](https://hackmd.io/@astrobiomike/BRAILLE-update-3-Feb-2021)
> - [https://hackmd.io/@astrobiomike/BRAILLE-notes-12-Dec-2020](https://hackmd.io/@astrobiomike/BRAILLE-notes-12-Dec-2020)

Metagenomic processing repository with data and code is at [OSF here](https://osf.io/uhk48/wiki/home/). All data are there, but not necessarily all of the processing and figures in these update docs.

## C-fixation and *Actinobacteria*
Maggie's talk mentioned that from the lipid profiles they anticipated Actinobacteria being there, and that we could see if they might be involved in C-fixation if we recovered any genomes of them. 

We do have 20 high-quality MAGs for them out of the 131 total MAGs that were recovered. And it seems that 7 of those 20 Actinobacteria MAGs have the genes for C-fixation through the CBB cycle 👍

<br>

<a href="https://i.imgur.com/ntumpd3.png"><img src="https://i.imgur.com/ntumpd3.png"></a>

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<br>

|||
|:---|--:|
|Total MAGs|131|
|Actinobacteria MAGs|20|
|Actinobacteria MAGs with C-fixation pathway |7|
|Other MAGs with C-fixation pathway|5|
|||

<br>

The 5 other MAGs are 1 Chloroflexota, 1 Gemmatimonadetes, and 3 Proteobacteria (D01-MAG-97, Alpha, family Acetobacteraceae; C05-MAG-88, Gamma, genus Panacagrimonas; C05-MAG-77, Gamma, genus Nevskia).


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### Phylogenomic tree of our Actinobacteria
Those with C-fixation pathway are marked.

<a href="https://i.imgur.com/2rUZAm5.png"><img src="https://i.imgur.com/2rUZAm5.png"></a>

[**Interactive tree here**](https://itol.embl.de/tree/13822922232360061616004973)

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### CBB cycle our buggers are equipped for
<a href="https://i.imgur.com/UY8Pfci.png"><img src="https://i.imgur.com/UY8Pfci.png"></a>

Overlain onto [this KEGG map](https://www.genome.jp/kegg-bin/show_pathway?map00710)

