--- tags: BRAILLE title: BRAILLE update (17-Mar-2021) --- [toc] --- >**Interactive function plots and tables are [here](https://astrobiomike.shinyapps.io/braille-mg/)**. --- # BRAILLE update (17-Mar-2021) > Previous update docs > - [https://hackmd.io/@astrobiomike/BRAILLE-update-24-Feb-2021](https://hackmd.io/@astrobiomike/BRAILLE-update-24-Feb-2021) > - [https://hackmd.io/@astrobiomike/BRAILLE-update-3-Feb-2021](https://hackmd.io/@astrobiomike/BRAILLE-update-3-Feb-2021) > - [https://hackmd.io/@astrobiomike/BRAILLE-notes-12-Dec-2020](https://hackmd.io/@astrobiomike/BRAILLE-notes-12-Dec-2020) Metagenomic processing repository with data and code is at [OSF here](https://osf.io/uhk48/wiki/home/). All data are there, but not necessarily all of the processing and figures in these update docs. ## C-fixation and *Actinobacteria* Maggie's talk mentioned that from the lipid profiles they anticipated Actinobacteria being there, and that we could see if they might be involved in C-fixation if we recovered any genomes of them. We do have 20 high-quality MAGs for them out of the 131 total MAGs that were recovered. And it seems that 7 of those 20 Actinobacteria MAGs have the genes for C-fixation through the CBB cycle 👍 <br> <a href="https://i.imgur.com/ntumpd3.png"><img src="https://i.imgur.com/ntumpd3.png"></a> <br> <br> ||| |:---|--:| |Total MAGs|131| |Actinobacteria MAGs|20| |Actinobacteria MAGs with C-fixation pathway |7| |Other MAGs with C-fixation pathway|5| ||| <br> The 5 other MAGs are 1 Chloroflexota, 1 Gemmatimonadetes, and 3 Proteobacteria (D01-MAG-97, Alpha, family Acetobacteraceae; C05-MAG-88, Gamma, genus Panacagrimonas; C05-MAG-77, Gamma, genus Nevskia). --- ### Phylogenomic tree of our Actinobacteria Those with C-fixation pathway are marked. <a href="https://i.imgur.com/2rUZAm5.png"><img src="https://i.imgur.com/2rUZAm5.png"></a> [**Interactive tree here**](https://itol.embl.de/tree/13822922232360061616004973) --- ### CBB cycle our buggers are equipped for <a href="https://i.imgur.com/UY8Pfci.png"><img src="https://i.imgur.com/UY8Pfci.png"></a> Overlain onto [this KEGG map](https://www.genome.jp/kegg-bin/show_pathway?map00710)